加味益气活血汤对急性心肌缺血及高脂血症模型小鼠的保护作用

发布时间：2018-04-25 11:47

本文选题：加味益气活血 + 急性心肌缺血　；参考：《延边大学》2014年硕士论文

【摘要】：目的：探讨加味益气活血汤(JIAWEIYIQIHUOXUE, JWYQ)对急性心肌缺血及高脂血症模型小鼠的保护作用。方法：①观察气管夹闭心肌缺血模型小鼠使用加味益气活血方时心电消失时间的变化与影响：40只昆明雄性小鼠以每组10只,按体重随机分为4组,分别为JWYQ大剂量组,JWYQ小剂量组,正常组,地奥组。正常组平时喂食普通小鼠饲料,其他组小鼠按灌胃量0.2ml/10g,每天灌胃JWYQ(39.OOg生药/kg,19.50g生药/kg),地奥心血康(0.195g/kg),连续20天后,在末次给药45Min后对小鼠进行腹腔注射麻醉(20%乌拉坦aq,1.2g/kg)。麻醉完成后用小动脉夹夹闭小鼠气管,观察记录心电消失时间。②50只昆明雄性小鼠以每组10只,按体重随机分为5组,分别为JWYQ大剂量组,JWYQ、剂量组,正常组,对照组,地奥组。正常组平时喂食普通小鼠饲料,其他组小鼠按灌胃量.0.2ml/10g,每天灌胃JWYQ(39.OOg生药/kg,19.50g生药/kg),地奥心血康(0.195g/kg),对照组灌胃等量蒸馏水,连续20天后,在末次给药45Min后除正常对照小组外,其余各小组均皮下注射Iso (0.015g/kg),建立急性心肌缺血模型,注射Iso注射液15min后,对5个小组小鼠置于密闭容器(250mi)中,分别记录其存活时间。③50只昆明雄性小鼠以每组10只,按体重随机分为5组,分别为JWYQ大剂量组,JWYQ小剂量组,正常组,对照组,辛伐他汀组。正常组平时喂食普通小鼠饲料,其它组均喂食高脂饲料。小鼠按灌胃量0.2ml/10g,每天灌胃JWYQ(39.OOg生药/kg,19.50g生药/kg),辛伐他汀(3mg/kg),连续20天后,在末次喂药24h之后,各小组老鼠进行摘眼球取血,分离出血清,进行高脂血症小鼠甘油三酯的对比测定。结果：①中药加味益气活血方可以有效延长气管夹闭实验小鼠的心电消失时间。②中药加味益气活血方可以有效延长实验小鼠注射Iso注射液诱发急性心肌缺血模型后在常压密闭容器内耐缺氧存活时间。③中药加味益气活血方可以有效降低高脂血症实验小鼠体内甘油三酯。使用大剂量的中药加味益气活血方延长气管夹闭后心电消失时间的能力与注射Iso急性心肌缺血模型后耐缺氧存活时间的能力强于地奥心血康胶囊；且使用大剂量的中药加味益气活血方降低高脂血症实验小鼠体内甘油三酯的能力也强于辛伐他汀胶囊。结论：本次实验研究表明加味中药益气活血方可以有效保护模型小鼠的急性心肌缺血,可以有效延长注射Iso诱发小鼠急性心肌缺血时密闭空间内耐缺氧的存活时间,有效延长小鼠常压密闭空间内耐缺氧存活能力,能有效降低高脂血症小鼠体内甘油三酯(TG)。
[Abstract]:Objective: To investigate the protective effects of JIAWEIYIQIHUOXUE (JWYQ) on acute myocardial ischemia and hyperlipidemia mice.
Methods: (1) observe the change and influence of the time of electrocardio disappearance of the rat model of myocardial ischemia model with Jiawei Yiqi Huoxue Recipe: 40 male mice in Kunming were divided into 4 groups according to the weight of 10 rats. They were the large dose group, the JWYQ small dose group, the normal group and the Austrian group. The normal group was fed with ordinary mice feed and the other groups. Mice were treated with gastric perfusion 0.2ml/10g, JWYQ (39.OOg /kg, 19.50g raw drug /kg) and Diao Xin Xue Kang (0.195g/kg) every day. After 20 days of continuous delivery, the mice were anesthetized by intraperitoneal injection (20% uran AQ, 1.2g/kg) after the last administration of 45Min (20% uran AQ, 1.2g/kg). After the anesthesia was completed, the mouse trachea was clamped with a small artery clamp, and the time of disappearance of electrocardiogram was observed. 50 Kunming males were recorded. 10 rats in each group were divided into 5 groups according to their weight. They were JWYQ large dose group, JWYQ, dose group, normal group, control group, and Diao group. The normal group was fed with ordinary mice feed, the other groups were given.0.2ml/10g of gastric perfusion, JWYQ (/kg, 19.50g /kg), Diao Xin Xue Kang (0.195g/kg), and the control group gavage. After 20 days of distilled water, after the last dose of 45Min, the other groups were subcutaneously injected with Iso (0.015g/kg) to establish an acute myocardial ischemia model. After the injection of Iso injection 15min, 5 groups of mice were placed in the closed container (250mi), and the survival time was recorded respectively. (3) 50 Kunming male mice were treated with 10 mice in each group. The body weight was randomly divided into 5 groups: the JWYQ large dose group, the JWYQ small dose group, the normal group, the control group, the simvastatin group. The normal mice were fed with ordinary mice feed and the other groups were fed with high fat feed. The mice were fed with 0.2ml/10g, and were fed with JWYQ (/kg, 19.50g /kg) and simvastatin (3mg/kg) every day for the last 20 days, at the last feeding. After 24h, each group of mice took blood from the eyeballs and separated the bleeding to determine the triglyceride level in the hyperlipidemic mice.
Results: (1) the traditional Chinese medicine supplemented Yiqi Huoxue recipe can effectively prolong the time of electrocardiogram disappearance in the experimental mice. (2) the prescription of Supplementing Qi Yiqi and activating blood can effectively prolong the survival time of the experimental mice induced by the injection of Iso injection to the acute myocardial ischemia model in the normal pressure closed vessel. It can effectively reduce triglyceride in hyperlipidemic mice.
The ability of using a large dose of Chinese medicine plus Yiqi Huoxue recipe to prolong the time of disappearance of electrocardiogram after tracheal clamping and the ability to endure hypoxia survival time after injection of Iso acute myocardial ischemia model is stronger than that of dioxin Kang capsule, and the ability to reduce the ability of triglycerides in the experimental mice of hyperlipidemia with a large dose of traditional Chinese medicine plus Yiqi Huoxue recipe. It's better than Simvastatin Capsules.
Conclusion: the experimental study shows that the supplemented Chinese medicine Yiqi Huoxue recipe can effectively protect the acute myocardial ischemia of the model mice, and can effectively prolong the survival time of hypoxia tolerance in the closed space of the mice induced by Iso, effectively prolong the viability of the hypoxia tolerance in the confined space of the mice, and effectively reduce the high fat blood. Triglyceride (TG) in the body of the mice.

【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5

【参考文献】