ORM1 A113G基因多态性对女性患者维库溴铵肌松效应的影响

发布时间：2018-04-26 15:19

本文选题：维库溴铵 + 神经肌肉阻滞　；参考：《临床麻醉学杂志》2015年04期

【摘要】：目的探讨ORM1 A113G基因多态性对女性患者维库溴铵肌松效应的影响。方法选择择期全麻下乳腺包块切除术贵州汉族女性患者107例,年龄18~65岁。术前采集外周静脉血5ml,使用天根血液基因组DNA提取试剂盒抽取DNA。采用3730XL DNA测序仪直接测序对ORM1A113G进行基因分型。麻醉诱导期间给予维库溴铵0.1mg/kg,用TOF-Watch加速度仪进行肌松监测。观察起效时间、时效、体内作用时间、恢复指数、TOF恢复到25%的时间。结果根据基因型将患者分为野生型纯合子组(AA组,n=71)、突变型杂合子组(AG组,n=35)和突变型纯合子组(GG组,n=1)。将AG组和GG组两种基因型合并为变异组。与AA组比较,变异组的时效和TOF恢复到25%时间均明显延长(P0.05);起效时间、恢复指数和体内作用时间差异无统计学意义。结论ORM1 A113G基因多态性可在一定程度上影响贵州汉族女性患者维库溴铵的肌松效应。
[Abstract]:Objective to investigate the effect of polymorphism of ORM1 A 113G gene on muscle relaxation of vecuronium bromide in female patients. Methods 107 female patients, aged 1865 years, underwent breast mass resection under elective general anesthesia in Guizhou Han nationality. The peripheral venous blood was collected 5 ml before operation and the genomic DNA extraction kit was used to extract DNA. The genotyping of ORM1A113G was carried out by direct sequencing with 3730XL DNA sequencer. Vecuronium bromide was given 0.1 mg / kg during anesthesia induction, and muscle relaxation was monitored by TOF-Watch accelerometer. The response time, time of action, time of action in vivo and recovery index of TOF were observed to be 25%. Results the patients were divided into wild type homozygote group (AA group), mutant heterozygote group (AG group) and mutant homozygote group (GG group). AG and GG genotypes were combined into variant group. Compared with AA group, the time of time and TOF recovery to 25% in the mutant group were significantly prolonged (P 0.05), but there was no significant difference in the onset time, recovery index and in vivo action time. Conclusion the polymorphism of ORM1 A113G gene may affect the muscle relaxation effect of vecuronium in Guizhou Han women.
【作者单位】：福建省立医院麻醉科;上海市浦东医院麻醉科;贵阳医学院麻醉学系;
【基金】：上海市卫计委基金(20134272)
【分类号】：R614

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