淫羊藿苷抗尿酸钠诱导的大鼠急性痛风性关节炎作用

发布时间：2018-04-28 07:09

本文选题：淫羊藿苷 + 尿酸钠　；参考：《中国实验方剂学杂志》2017年11期

【摘要】：目的:观察淫羊藿苷(icariin,ICA)对尿酸钠诱导的大鼠急性痛风性关节炎(gouty arthritis,GA)的影响,并探讨其相关的作用机制。方法:SD大鼠72只,雄雌各半,随机分为正常组,模型组,淫羊藿苷低、中、高剂量组(20,40,80 mg·kg~(-1)),秋水仙碱组(1.5 mg·kg~(-1)),每组12只,按分组预给药5 d后在大鼠右踝关节腔内注射3.0%尿酸钠溶液100μL复制大鼠GA模型,继续给药48 h,期间测量右踝关节周径并作步态评分。造模48 h后麻醉动物,苏木素-伊红染色观察关节腔积液涂片和关节滑膜白细胞浸润情况,酶联免疫吸附测定(ELISA)测定关节囊和滑膜组织中白细胞介素-1β(IL~(-1)β),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α)和前列腺素E_2(PGE_2)含量。蛋白质免疫印迹(Western blot)测定关节囊和滑膜组织环氧化酶-2(COX-2)蛋白含量。结果:模型组大鼠关节周围组织肿胀,步态跛行,关节积液和滑膜组织中大量白细胞浸润,炎症因子IL~(-1)β,IL-6,TNF-α均显著高于正常组,PGE_2含量明显升高(P0.05),关节囊和滑膜组织COX-2蛋白表达升高(P0.05);与模型组比较,ICA低、中、高剂量组可明显改善GA模型大鼠关节肿胀和步态异常,显著降低白细胞浸润和IL~(-1)β,IL-6,TNF-α,PGE_2含量,明显降低COX-2蛋白表达(P0.05)。结论:ICA可通过抑制炎症反应改善尿酸钠诱导的大鼠急性GA症状。
[Abstract]:Aim: to observe the effect of icariin Icariin (ICA) on acute gouty arthritis GA1 induced by sodium uric acid in rats, and to explore its mechanism. Methods 72 male and half male Sprague-Dawley rats were randomly divided into normal group, model group, low icariin group, middle and high dose group (20 ~ 4080 mg 路kg ~ (-1) and colchicine group (n = 12), and colchicine group (n = 12). The rat GA model was established by injecting 3.0% sodium uric acid solution into the right ankle joint of rats after 5 days of preadministration according to the group. The rats were given the drug for 48 hours. The circumference of the right ankle joint was measured and the gait score was scored. After 48 hours of modeling, hematoxylin eosin staining was used to observe the joint effusion smear and synovial leukocyte infiltration in anesthetized animals. The contents of interleukin-1 尾 -l), interleukin-6 (IL-6), tumor necrosis factor- 伪 (TNF- 伪) and prostaglandin E2PGE2) in the synovial tissues were determined by Elisa. Western blot was used to detect the content of cyclooxygenase-2 (COX-2) protein in synovium and articular capsule. Results: in the model group, the periarticular tissue swelling, gait claudication, massive leukocyte infiltration in joint effusion and synovial tissue were observed. The level of IL-6 TNF- 伪 was significantly higher than that of PGE2 in normal group, and the expression of COX-2 protein in articular capsule and synovial tissue was higher than that in control group, which was lower than that in model group, and the swelling of joints and abnormal gait in GA model rats were significantly improved in high dose group. The leukocyte infiltration and IL-6TNF- 伪 PGE2 content were significantly decreased, and the expression of COX-2 protein was significantly decreased (P 0.05). ConclusionICA can ameliorate acute GA symptoms induced by sodium uric acid by inhibiting inflammatory reaction in rats.
【作者单位】：遵义医学院基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室;
【基金】：贵州省淫羊藿开发利用科技创新人才团队项目[黔科合人才团队(2015)4023号]
【分类号】：R285.5

【参考文献】