丙泊酚对脑缺血再灌注大鼠脑组织Homer-1a表达的影响及其机制

发布时间：2018-04-28 14:24

  本文选题：缺血再灌注损伤 + 脑组织　； 参考：《山东医药》2015年12期

【摘要】：目的观察丙泊酚对脑缺血再灌注大鼠脑组织Homer-1a表达的影响,并探讨其机制。方法雄性SD大鼠120只,随机分为假手术组、造模组、丙泊酚组,每组40只。模型组、丙泊酚组线栓法建立脑缺血再灌注模型,假手术组仅行血管分离处理不插入线栓;造模后30 min,丙泊酚组腹腔注射丙泊酚10 mg/100 g,假手术组和对照组均腹腔注射等体积生理盐水。给药处理3 h、24 h、72 h、7 d分别进行神经功能评分,采用Real-time PCR法检测脑组织Homer-1a mRNA,应用酶联免疫吸附法检测血清S-100β。结果与假手术组比较,模型组和丙泊酚组4个时间点神经功能评分、血清S-100β水平均增加(P均0.05),以模型组增高更显著(P均0.05)。与假手术组相比,模型组在3、24 h时间点脑组织Homer-1a mRNA降低(P均0.05);丙泊酚组4个时间点脑组织Homer-1a mRNA均较其他两组升高(P均0.05)。在所有大鼠样本中,脑组织Homer-1a mRNA表达与血清S-100β呈负相关(r=-0.939,P0.01)。结论丙泊酚麻醉可通过诱导脑组织Homer-1a mRNA表达发挥对脑缺血再灌注大鼠的脑保护作用,该作用可能与其降低血清S-100β水平有关。
[Abstract]:Objective to observe the effect of propofol on the expression of Homer-1a in cerebral ischemia reperfusion rats and to explore its mechanism. Methods 120 male SD rats were randomly divided into sham-operated group, model group and propofol group with 40 rats in each group. Model group, propofol group was used to establish cerebral ischemia-reperfusion model, sham-operation group was only treated with vascular separation and did not insert thread embolus. After 30 minutes, propofol was injected intraperitoneally with propofol for 10 mg/100 g, and normal saline was injected intraperitoneally in sham operation group and control group. The nerve function scores were evaluated by Real-time PCR method and serum S-100 尾 was detected by enzyme-linked immunosorbent assay (Elisa). Results compared with the sham operation group, the nerve function scores and serum S-100 尾 levels in the model group and propofol group were increased at 4 time points (P < 0.05), especially in the model group (P = 0.05). Compared with the sham operation group, the Homer-1a mRNA of brain tissue in the model group decreased by 0.05 at 3h at 24 h, and the Homer-1a mRNA in the brain tissue in the propofol group at four time points was higher than that in the other two groups (P < 0.05). There was a negative correlation between the expression of Homer-1a mRNA and serum S-100 尾 in all rat samples. Conclusion propofol anesthesia can induce the expression of Homer-1a mRNA in brain tissue and play a protective role in cerebral ischemia-reperfusion rats, which may be related to the decrease of serum S-100 尾 level.
【作者单位】： 铁岭市中心医院;大连医科大学附属第一医院;
【基金】：国家自然科学基金资助项目(81371454)
【分类号】：R285.5
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本文编号：1815636