重复异丙酚麻醉对新生大鼠海马细胞凋亡及远期学习记忆能力的影响

发布时间：2018-04-29 00:20

本文选题：二异丙酚 + 细胞凋亡　；参考：《北京大学学报(医学版)》2017年02期

【摘要】：目的:探讨重复异丙酚麻醉对新生大鼠海马细胞凋亡及远期学习记忆能力的影响。方法:清洁级SD大鼠45只,7 d龄,雄性,随机分为3组(n=15):对照组(C组)腹腔注射脂肪乳剂7.5 m L/kg,每天1次,连续7 d;反复注射异丙酚组(P1组)腹腔注射异丙酚75 mg/kg,每天1次,连续7 d;单次注射异丙酚组(P2组)腹腔注射脂肪乳剂7.5 m L/kg,每天1次,连续6 d,第7天注射异丙酚75 mg/kg。每组取5只大鼠,于苏醒后即刻抽取动脉血样行血气分析。异丙酚给药结束后2周时采用Morris水迷宫实验测试大鼠的学习记忆功能,之后处死大鼠,免疫组织化学法检测大鼠海马神经元特异性核蛋白(neuron-specific nucleoprotein,Neu N)的表达,Western blot检测大鼠海马组织中caspase-3的表达。结果:3组大鼠动脉血气指标差异无统计学意义。与C组相比,P1组大鼠第5天寻找平台潜伏期延长、寻找平台路径增长,第6天目标象限探索时间缩短、穿环次数减少(P0.05);而P2组的寻找平台潜伏期、寻找平台路径长度、探索时间、穿环次数与C组相比差异无统计学意义。与C组相比,P1组大鼠海马组织内Neu N阳性细胞数量减少、caspase-3表达增强;P2组大鼠海马内Neu N阳性细胞数量、caspase-3的表达与C组相比差异无统计学意义。结论:重复异丙酚麻醉可降低新生大鼠的远期学习记忆能力,可能与其增加海马内的细胞凋亡有关,单次异丙酚麻醉对新生大鼠的海马细胞凋亡及远期学习记忆能力没有显著影响。
[Abstract]:Aim: to investigate the effects of repeated propofol anesthesia on hippocampal apoptosis and long term learning and memory in neonatal rats. Methods: 45 male SD rats of 7 days old were randomly divided into 3 groups: control group C): fat emulsion 7.5 mL / kg once a day for 7 days, and propofol 75 mg / kg intraperitoneally once a day in the propofol group. For 7 days, single injection of propofol and propofol (P _ 2 group) were treated by intraperitoneal injection of fat emulsion 7.5 mL / kg, once a day for 6 days, and then 75 mg / kg / kg of propofol was injected on the 7th day. Five rats were taken from each group and arterial blood samples were collected immediately after awakening for blood gas analysis. Morris water maze test was used to test the learning and memory function of rats 2 weeks after the administration of propofol, and then the rats were killed. Immunohistochemical method was used to detect the expression of neuron-specific nucleoprotein (Neu N) in rat hippocampal neurons. Western blot was used to detect the expression of caspase-3 in rat hippocampal tissue. Results there was no significant difference in arterial blood gas index among the three groups. Compared with group C, the latency of finding platform in group P1 was prolonged on day 5, and the path of finding platform was increased, the exploration time of target quadrant was shortened on day 6, and the number of loop piercing was decreased (P0.05A), while in group P2, the latency of finding platform and the length of path of finding platform were found. There was no significant difference between group C and group C in exploration time and number of piercing. Compared with group C, the number of Neu N positive cells in hippocampal tissue decreased and the expression of caspase-3 in hippocampal tissue increased in group P 2. There was no significant difference in the number of positive cells of Neu N and caspase-3 between group P 2 and group C. Conclusion: repeated propofol anesthesia can reduce the long-term learning and memory ability of neonatal rats, which may be related to the increase of apoptosis in hippocampus. Single propofol anesthesia had no significant effect on hippocampal apoptosis and long-term learning and memory in neonatal rats.
【作者单位】：北京大学第三医院麻醉科;
【基金】：国家自然科学基金(21342016)资助~~
【分类号】：R726.1

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