法舒地尔干预对阿霉素致大鼠心肌损伤的实验研究

发布时间：2018-04-30 01:33

本文选题：法舒地尔 + 阿霉素　；参考：《广西医科大学》2014年硕士论文

【摘要】：目的：研究法舒地尔（Fas）干预对阿霉素（ADM）致大鼠血清肌钙蛋白（cTnI）、ATP、ADP、光镜及电镜超微结构的变化和影响，从而探讨Fas干预对ADM诱导心肌损伤的保护作用，为癌症患者的围术期心肌保护提供一定的实验依据。方法：将体重200g左右的32只雌性大鼠随机分成4组，每组8只。（1）A组（空白对照）：于实验第1、3、5、7、9、11天（9：00AM）腹腔注射生理盐水，每次10ml/kg；第14天开始，每次10ml/kg/天，连续6天。（2）B组（ADM+生理盐水）：于实验第1、3、5、7、9、11天（9：00AM）腹腔注射ADM2.5mg/kg；第14天起，每日生理盐水10ml/kg/次，连续6天。（3）C组（ADM+Fas10mg/kg/天）：前6次给药同B组，于实验第14天起注射Fas，每次10mg/kg/天，连续6天。（4）D组（ADM+Fas2mg/kg/天）：前6次给药同B组，于实验第14天起注射Fas，每次2mg/kg/天，连续6天。在最后一次给药后第4天，腹腔注射10%的水合氯醛麻醉大鼠。分别在实验开始前（T1）、注射ADM48小时后（T2）、注射Fas24小时后（T3）、麻醉后（T4）四个时间点采血，检测血清cTnI水平；检测4组小鼠心肌ATP、ADP含量。留取心肌组织观察光镜、电镜下的病理变化。结果：(1)在T2时点，B、C、D三组大鼠的cTnI值与A组相比均显著升高（P0.01），而B、C、D三组之间无显著差异（P0.05）；在T3时点，C、D两组与B组的cTnI值比较有明显下降（P0.01)，而C、D两组间也存在显著差异（P0.01）；在T4时点C、D两组与B组的cTnI值比较同样有明显下降（P0.01)，但C、D两组间无明显差异（P0.05）；C、D两组在T2时点cTnI水平最高；而C组在T3、T4时点中的cTnI值有统计学意义（P0.05）；D组在T3、T4时点中的cTnI值无统计学差异（P0.05）。（2）四组大鼠的ADP值比较均有统计学差异（P0.05）；而C、D两组间的ATP值比较无统计学差异（P0.05），其余各组间的比较有显著差异（P0.05）。（3）大鼠的心肌损伤病理评分，B、C、D组与A组相比均升高（P0.01）；但与B组相比，干预后的C、D两组明显有所改善，，评分较低（P0.05）；C、D间无显著差异（P0.05）。（4）心肌电镜超微结构，除A组表现正常，其余三组均发生严重改变，其中B组损伤表现最为严重，C组优于D组，但仍然不及A组超微结构正常、清晰。结论：（1）ADM导致大鼠心肌损伤，血清cTnI水平显著上升，心肌ADP、ATP含量明显下降；（2）Fas干预ADM诱导后的大鼠，血清cTnI水平下降，心肌ATP、ADP含量明显升高，病理评分明显下降，心肌超微结构得到改善，提示Fas对ADM诱导的心肌损伤有一定的保护作用。
[Abstract]:Objective: to study the changes and effects of fascia on the changes and ultrastructure of troponin cTnInAP-ATPase in serum of rats induced by adriamycin (ADM), so as to explore the protective effect of Fas intervention on myocardial injury induced by ADM. To provide a certain experimental basis for perioperative myocardial protection of cancer patients. Methods: Thirty-two female rats weighing about 200g were randomly divided into 4 groups, 8 rats in each group, 8 rats in each group (control group: 9: 00 amm, 911 days after experiment), 10 ml / kg of normal saline was injected intraperitoneally, and 14 days later, every 10ml/kg/ day. In group B, adm 2.5 mg / kg was injected intraperitoneally on the 1st day of the experiment, followed by intraperitoneal injection of ADM 2.5 mg / kg on the 14th day. Daily normal saline 10ml/kg/ was given daily for 6 days. Group C was given the same drug as group B on the 14th day of the experiment. Group D was given Fas2mg/kg/ for 6 consecutive days: the first 6 times were given to the same group as group B, and 2mg/kg/ was injected on the 14th day of the experiment for 6 days, each time for 6 days. On the fourth day after the last administration, 10% chloral hydrate was injected intraperitoneally to anesthetized rats. The blood samples were collected at four time points before the beginning of the experiment, after ADM48 injection, after injection of Fas24, and after anesthesia, respectively. The levels of serum cTnI and the content of Fas24 in myocardium of the four groups were detected. Myocardial tissue was taken to observe the pathological changes under light microscope and electron microscope. Results at T2, the cTnI values of the three groups were significantly higher than those of the group A, but there was no significant difference among the three groups. The cTnI value of the two groups was significantly lower than that of the group B at T3 time, and there was a significant difference between the two groups, and there was also a significant difference between the two groups. At T4, the cTnI values of group C and group B also decreased significantly, but there was no significant difference between group C and group D in cTnI level at T 2, but there was no significant difference between group D and group C (P 0.05) at T _ 2 time, but the level of cTnI was the highest in group C _ (0.05) and C _ (D) at T _ 2. There was no significant difference in cTnI value between group C and group D at T3 / T4. (P < 0.05) the ADP value of group C was significantly different from that of group C (P < 0.05), but there was no significant difference in ATP value between the two groups (P0.05), while the other two groups had no significant difference in ATP value between the two groups (P0.05), while the ADP value of group C had no statistical difference in T3 / T4 time (P < 0.05); the ADP value of group C was significantly different from that of group C (P < 0.05); and there was no significant difference in ATP value between the two groups. The pathological score of myocardial injury was significantly higher in group B than in group A, but it was higher in group B than in group B, but it was significantly higher in group B than in group B, but it was significantly higher in group B than in group B, but it was significantly higher in group B than that in group A, but it was higher in group B than in group B. There was no significant difference between the two groups after intervention. The ultrastructure of myocardium in group A was normal, and the other three groups had serious changes. Group B had the most severe injury than group D. The group C had the most serious injury than the group D, and the ultrastructure of myocardium in group C was better than that in group D. the ultrastructure of myocardium in group C was more serious than that in group D, except for group A, which was normal and the other three groups had serious changes. However, the ultrastructure of group A was still not normal and clear. Conclusion the level of cTnI in serum of rats induced by cTnI was significantly increased, and the level of cTnI in serum decreased, and the pathological score was significantly decreased in rats after ADM was induced by Fas. Myocardial ultrastructure was improved, suggesting that Fas has a protective effect on myocardial injury induced by ADM.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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