吸入外源性硫化氢对心跳骤停复苏大鼠脑组织炎症反应及生存率的影响

发布时间：2018-05-04 04:04

本文选题：心肺复苏术 + 硫化氢　；参考：《临床麻醉学杂志》2015年07期

【摘要】：目的探讨外源性硫化氢(H2S)对心跳骤停复苏大鼠脑组织炎症反应及生存率的影响。方法健康雄性SD大鼠90只,体重260~280g。按随机原则分为三组:对照组(Sham组,n=20):吸入麻醉药物诱导,气管插管,动静脉置管,但不进行心跳骤停及复苏操作。其余大鼠经气管插管后进行窒息性心跳骤停并复苏,自主循环恢复(ROSC)后随机分为两组:心跳骤停复苏组(CA组,n=35)、H2S组(n=35)。H2S组大鼠复苏恢复自主循环(ROSC)后立即吸入80ppm H2S 60min。每组取15只大鼠,于ROSC后(sham组麻醉后)3、24和72h时断头取脑组织,测定脑组织中白细胞介素10(IL-10)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)及髓过氧化物酶(MPO)的含量。采用胶带移除实验评估大鼠ROSC后第14d的神经功能。记录ROSC后14d内3组大鼠的存活数量,随后断头取脑,计数海马CA1区存活神经元个数。结果 ROSC后3h时H2S组IL-10表达量明显高于Sham和CA组(P0.05);CA和H2S组在ROSC后24h时脑组织IL-1β表达量,ROSC后3、24h时脑组织TNF-α表达量明显高于Sham组,且CA组明显高于H2S组(P0.05);ROSC后72h时CA组MPO活性明显高于Sham组和H2S组(P0.05)。CA组及H2S组存活神经元数量明显少于Sham组,且CA组明显少于H2S组(P0.05)。ROSC后14dSham和H2S组存活率明显高于CA组(P0.05)。结论大鼠心跳骤停复苏后早期吸入外源性80ppm H2S可提高心肺复苏后14d生存率,其机制可能与抑制脑组织炎症反应,改善海马CA1区神经元存活有关。
[Abstract]:Objective to investigate the effects of exogenous hydrogen sulfide (H _ 2S) on the inflammatory response and survival rate of brain tissue in rats with cardiac arrest resuscitation. Methods 90 healthy male Sprague-Dawley rats weighing 260 ~ 280 g. They were randomly divided into three groups: control group (Sham group): inhaled anesthetic induction, tracheal intubation, arteriovenous catheterization, but no cardiac arrest and resuscitation. The rest rats were randomly divided into two groups after asphyxiating cardiac arrest and resuscitation after tracheal intubation. After spontaneous circulation was recovered, the rats in the cardiac arrest and resuscitation group were randomly divided into two groups: the CA group, the CA group and the H2S group. The rats in the H2S group were inhaled 80ppm H2S for 60 mins immediately after resuscitation and recovery of autonomic circulation. Brain tissue was taken from 15 rats in each group at 24 and 72 hours after anesthesia in sham group after ROSC. The contents of interleukin 10 IL-10, IL-1 尾 1 尾, TNF- 伪 and myeloperoxidase (MPO) in brain tissue were measured. The nerve function of rats was evaluated by tape removal test on day 14 after ROSC. The survival numbers of the three groups were recorded within 14 days after ROSC, and the number of surviving neurons in the hippocampal CA1 area was counted. Results the expression of IL-10 in H2S group was significantly higher than that in Sham group and CA group at 3 h after ROSC. The expression of IL-1 尾 in brain tissue in H2S group was significantly higher than that in Sham group at 24 h after ROSC. The activity of MPO in CA group was significantly higher than that in H2S group at 72 h after P0.05 roSC. The number of surviving neurons in CA group was significantly higher than that in Sham group and H2S group, and the number of surviving neurons in CA group was significantly lower than that in Sham group, and the survival rate of 14dSham and H2S group in CA group was significantly higher than that in CA group. Conclusion early inhalation of exogenous 80ppm H2S after cardiopulmonary resuscitation can improve the survival rate of 14 days after cardiopulmonary resuscitation in rats. The mechanism may be related to the inhibition of inflammation in brain tissue and the improvement of neuron survival in CA1 area of hippocampus.
【作者单位】：哈尔滨医科大学附属第二医院麻醉科黑龙江省麻醉与危重病学重点实验室黑龙江省高校麻醉基础理论与应用研究重点实验室;
【基金】：国家自然科学基金(81372026,81000822)
【分类号】：R614

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