不同训练对大鼠骨骼肌活性氧阈与乳酸阈影响变化的研究

发布时间：2018-05-06 02:28

本文选题：递增负荷 + 自由基　；参考：《河北师范大学》2017年硕士论文

【摘要】：研究目的:在递增负荷至力竭运动中机体内活性氧(ROS)与血液中乳酸(LD)有着类似突增点的动态变化规律,且浓度变化与运动负荷有密切关系。ROS突增点(阈)表示ROS生成与抗氧化清除的失衡点,对氧化损伤监控有重要的应用价值。本文通过对完成同等条件递增负荷运动的无训练组和有训练组大鼠进行对比,验证ROS阈的客观存在性,并观察有训练组与无训练组大鼠相比在完成同等条件递增负荷运动时血液和骨骼肌中ROS阈是否会出现后移的的现象,同时观察血液中LD与肌酸激酶(CK)的动态变化,为深入探讨机制提供实验证据。研究方法:选取6周龄雄性Wistar大鼠42只,随机分为无运动训练组(E6组,再分为6个亚组,每亚组3只,共18只);有氧运动训练组(E8组,再分为8个亚组,每亚组3只,共24只,进行3周游泳训练)。E8组游泳训练结束24小时后,两组大鼠进行同等条件的一次性递增负荷力竭游泳运动,第1级负荷从无负重开始,之后每级负荷增加大鼠体重的1%,每级游泳5min。E6组设6级负荷,E8组设8级负荷。各亚组大鼠完成相应负荷后即刻麻醉,取血,取比目鱼肌、红腓肠肌、白腓肠肌,试剂盒检测血液和肌组织中的丙二醛(MDA)、H_2O_2、LD含量和CK、SOD活力。研究结果:1、两组大鼠在完成同等条件的递增负荷运动时,血液的MDA、H_2O_2、LD含量及SOD、CK活力变化:(1)血液中MDA、H_2O_2含量及CK活力E6组中第6级负荷与第5级负荷相比均有非常显著性差异(P0.01),而E8组中第7级负荷与第6级负荷相比均具有显著性差异(P0.05)。(2)血液中LD含量E6组第5级负荷与第4级负荷相比有非常显著性差异(P0.01),E8组第6级负荷与第5级负荷有显著性差异(P0.05)。(3)血液中SOD的活力E6组、E8组每一级负荷与前一级负荷相比较都无显著性差异(P0.05)。2、两组大鼠在完成同等条件的递增负荷运动时,比目鱼肌、红腓肠肌、白腓肠肌的MDA、H_2O_2含量及SOD活力变化:(1)MDA含量:E6组中比目鱼肌、红腓肠肌、白腓肠肌第6级负荷与第5级负荷相比均有显著性差异(P0.01,P0.01,P0.05);在E8组中红腓肠肌、白腓肠肌第8级负荷与第7级负荷相比含量均有非常显著性差异(P0.01),比目鱼肌第7级负荷与第6级负荷相比有显著性差异(P0.05)。(2)SOD活力:E6组和E8组三种肌肉类型中每级负荷与前一级负荷相比均无显著性差异(P0.05)。(3)H_2O_2含量:E6组中比目鱼肌、红腓肠肌第6级负荷与第5级负荷相比均有显著性差异(P0.05,P0.01),白腓肠肌第5级负荷与第4级负荷相比有显著性差异(P0.01);在E8组中比目鱼肌第7级负荷与第6级负荷相比有显著性差异(P0.01);红腓肠肌、白腓肠肌第8级负荷与第7级负荷相比均有显著性差异(P0.01,P0.05)。研究结论:1、在完成相同条件递增负荷运动时,E6组、E8组大鼠血液与肌组织中ROS阈均客观存在;且血液中ROS阈滞后于血液乳酸阈的出现。2、E8组大鼠血液与肌肉中ROS阈出现要滞后于E6组大鼠。这可能是由于有氧训练增强了线粒体在氧化磷酸化有氧供能水平和提高了抗氧化的能力。3、血液中CK活力突增点出现在血乳酸阈之后,与血液中ROS阈一致。间接说明ROS阈对应的运动负荷可能是加剧运动氧化损伤的临界负荷,导致CK从细胞内流出到血液中的活力显著升高。
[Abstract]:Research purposes: the dynamic changes of reactive oxygen species (ROS) and lactate (LD) in the body are similar to those of the blood, and the concentration changes are closely related to the movement load, which is closely related to the.ROS abrupt point (threshold) indicating the imbalance point between the formation of ROS and the antioxidant clearance, which is of great value for the monitoring of oxidative damage. The objective existence of ROS threshold was verified by comparison between the non training group and the training group, and the phenomenon of whether the ROS threshold in the blood and skeletal muscles in the training group was compared with the untrained group was observed and observed at the same time. At the same time, the LD and the LD in the blood were observed. The dynamic changes of creatine kinase (CK) provide experimental evidence for the in-depth study of the mechanism. Methods: 42 rats of 6 Zhou Lingxiong sex Wistar rats were selected and randomly divided into non exercise training group (group E6, 6 subgroups, 3 in each subgroup, 18); the aerobic exercise training group (group E8, again divided into 8 subgroups, 3 in each subgroup, 24 swimming training for 3 weeks).E After 24 hours of swimming training in the 8 groups, the two groups of rats were given the same condition for one time increasing load exhaustion swimming, and the first level load started from no weight bearing, and the weight of rats in each level was 1%, the 5min.E6 group of each level set up 6 levels, and the group E8 had 8 class load. The rats in each subgroup completed the immediate anesthesia after the corresponding load, taking the blood, taking the ratio. The gastrocnemius, red gastrocnemius, and white gastrocnemius were used to detect the malondialdehyde (MDA), H_2O_2, LD content and CK, SOD activity in blood and muscle tissue. The results were as follows: 1, the changes of MDA, H_2O_2, LD content, SOD and CK activity in the two groups of rats were performed at the same condition of increasing load exercise: (1) MDA in the blood, H_2O_2 content and sixth levels in the vitality group. There were significant differences in the load compared with the fifth level load (P0.01), while the seventh level load in the group E8 had significant differences (P0.05). (2) there was a significant difference in the E6 Group Fifth load in the E6 group of the blood and the fourth grade load (P0.01), and there was a significant difference (P0.05) between the sixth class load and the fifth load (P0.05) in the E8 group. (3) blood. There was no significant difference in the activity of SOD in group E6, and there was no significant difference (P0.05).2 for each level of E8 in group E8. The MDA of soleus, red gastrocnemius, white gastrocnemius, H_2O_2 content and SOD activity in the two groups of rats were completed in the same condition of incremental load exercise: (1) MDA content: the soleus muscle, the red gastrocnemius muscle, and the white gastrocnemius muscle sixth in the E6 group. There were significant differences (P0.01, P0.01, P0.05) in the grade load compared with the fifth stage load. In group E8, the red gastrocnemius muscle, the white gastrocnemius eighth load and the seventh stage load were significantly different (P0.01), compared with the sixth stage load (P0.05). (2) SOD activity: three muscle groups in E6 and E8 groups. There was no significant difference (P0.05) in each stage load in the model (3) H_2O_2 content: there were significant differences between the soleus muscle and the sixth grade load of the red gastrocnemius muscle (P0.05, P0.01) in group E6 (P0.05, P0.01), and the ratio of the white gastrocnemius muscle load to the fourth load (P0.01); in the E8 group, the seventh level load of the soleus muscle was in the E8 group. There was significant difference (P0.01) compared with the sixth level load (P0.01, P0.05) in the red gastrocnemius muscle and the white gastrocnemius eighth load (P0.05). 1. In the completion of the same condition increasing load exercise, the ROS threshold in the blood and the muscle group of the E8 group was all objective, and the ROS threshold in the blood was lagging behind the blood milk. The emergence of acid threshold in.2, ROS threshold in blood and muscle in group E8 rats is lagging behind group E6 rats. This may be due to aerobic training enhanced the mitochondrial oxygen supply level and the ability to increase the antioxidant capacity.3. The sudden increase of CK activity in the blood appears at the threshold of blood lactate, which is consistent with the ROS threshold in the blood. The ROS threshold is indirectly explained. The corresponding exercise load may be aggravated by the critical load of oxidative injury, resulting in a significant increase in the activity of CK from the cells to the blood.

【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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