解毒祛疣方对HPV感染表皮中朗格汉斯细胞的影响

发布时间：2018-05-09 12:30

  本文选题：跖疣 + HPV　； 参考：《南京中医药大学》2014年硕士论文

【摘要】：目的：跖疣是临床上常见的,好发于足底的寻常疣。西医对于多发性跖疣缺乏满意疗法。迅速有效地消除疣体,控制疼痛,减少复发率,是从事皮肤科工作的临床医生急切需要解决的重要课题。本课题以中医基础理论为指导,结合导师临床经验总结解毒祛疣方,通过实验观察解毒祛疣方对HPV感染表皮中朗格汉斯细胞的影响,为临床治疗提供一定的理论依据。 方法：8例HPV感染的尖锐湿疣(CA)患者均为我院皮肤科门诊患者,均为初发患者,未经过任何治疗。对照组：8例对照为同时期我院男科包皮手术非CA患者。征得患者知情同意后,局部消毒麻醉,分别切取疣体／正常组织各8例,每例组织等分为10份。其中一份直接10%甲醛溶液固定,石蜡包埋,将9份真皮侧向下,放入少量RPMI-1640培养液及浓度分别为0.025g/ml、0.05g/ml的药液,只将疣体底部浸于培养液中。分别以37℃℃、42℃℃和45℃三个温度照射30mmin后,PBS冲洗后,将疣体全部浸于RPMI-1640培养液,37℃C培养箱孵育12小时后将组织取出,10%甲醛溶液固定,石蜡包埋。常规石蜡切片,免疫组化染色后,观察LC形态变化,数量变化。 结果：1.解毒祛疣方治疗后HPV感染表皮中LC形态发生变化,细胞结构不完整,树突状结构减少、变短或消失。2.不同温热、中药浓度治疗后,表皮内LC的数量变化,随温度、浓度升高,LC数量逐渐减少,差异具有显著性(P0.01)；HPV感染组织比正常组织表皮内LC减少更明显,也随温度、浓度变化而变化。正常人和HPV感染组织相同温度、浓度之间的LC数量经统计学处理,也具有显著性差异(P0.01)。
[Abstract]:Objective: metatarsal warts are common in clinic. Western medicine lacks satisfactory treatment for multiple warts. It is urgent for clinicians engaged in dermatology to eliminate warts quickly and effectively, control pain and reduce recurrence rate. Under the guidance of the basic theory of traditional Chinese medicine, combined with the tutor's clinical experience, the author summarized the prescription of detoxification and dispelling warts, observed the effect of jiedu Quyoufang on Langerhans cells in HPV infected epidermis, and provided some theoretical basis for clinical treatment. Methods eight cases of HPV infected condyloma acuminatum were all outpatients in dermatology department of our hospital. The control group consisted of 8 cases of non-CA patients undergoing male prepuce surgery in the same period. After obtaining the informed consent of the patient, 8 cases of warts and 8 cases of normal tissue were cut out, and each case was divided into 10 parts. One of them was immobilized in 10% formaldehyde solution and embedded in paraffin wax. Nine parts of dermis were placed sideways down into a small amount of RPMI-1640 medium and 0.025 g / ml of 0.05 g / ml respectively. The verruca was only immersed in the medium at the bottom of the verruca. The warts were incubated in RPMI-1640 incubator at 37 鈩，

本文编号：1866019