利多卡因两种给药途径对兔蛛网膜下腔出血的脑保护作用

发布时间：2018-05-12 09:47

本文选题：给药途径 + 利多卡因　；参考：《四川大学学报(医学版)》2017年01期

【摘要】：目的比较利多卡因两种给药途径对蛛网膜下腔出血(SAH)的脑保护作用。方法 40只新西兰大白兔随机分为假手术(sham)组、SAH模型组(SAH组)、利多卡因静脉治疗组(L1组)及利多卡因枕大池治疗组(L2组),每组各10只。麻醉下采用自体动脉血注入枕大池制备SAH模型,sham组注入等量生理盐水(NS);30min后sham组、SAH组及L1组的动物,再次从枕大池注入0.3mL NS,L1组同时静脉给予0.3mL 2%利多卡因治疗,L2组从枕大池注入0.3mL 2%利多卡因治疗。所有动物72h后处死。分别于造模型前、治疗后72h采用ELISA法检测血清白介素-6(IL-6)水平;72h后取脑基底动脉以及海马组织行常规HE染色及C-fos免疫组化染色,测定基底动脉腔面积(AA)和直径(AD)、海马正常神经元密度(NNDHP)、C-fos阳性细胞数目(CCPHP)。结果各组动物造模前血清IL-6水平差异均无统计学意义(P0.05),治疗后72h各组IL-6水平均较术前高(P0.05),SAH及L1组高于sham组和L2组(P0.05)。SAH及L1组基底动脉的AA、AD及海马NNDHP比假手术sham组和L2组减少(P0.05),而SAH及L1组海马CCPHP比sham组和L2组增多(P0.05)。结论同剂量利多卡因经枕大池注入比静脉注入对兔蛛网膜下腔出血的脑保护效果好。
[Abstract]:Objective to compare the brain protective effects of lidocaine on SAH in subarachnoid hemorrhage. Methods Forty New Zealand white rabbits were randomly divided into two groups: sham group (n = 10) and lidocaine cistern group (n = 10). Under anesthesia, the SAH model was made by injecting autologous arterial blood into the cistern of the occipital cistern. The rats in the sham group were injected with the same amount of normal saline for 30 minutes, and the animals in the sham group and the L1 group were injected with the same amount of normal saline. 0.3mL 2% lidocaine was injected intravenously into the 0.3mL NSN L1 group from the cistern of the occipital cistern. 0.3mL 2% lidocaine was injected into the cistern of the occipital cistern in the L2 group. All animals were killed after 72 hours. The serum interleukin-6 (IL-6) level was detected by ELISA method before and 72 hours after treatment. The cerebral basilar artery and hippocampal tissue were taken out for routine HE staining and C-fos immunohistochemical staining. The area of basilar artery (AAA), the diameter of ADI and the number of NNDHP- C-fos positive cells in hippocampal normal neurons were measured. Results there was no significant difference in serum IL-6 levels in all groups before model making. The levels of IL-6 in all groups were higher than those in sham group and L 2 group at 72 h after treatment compared with those in sham group and L 1 group, and were higher than those in sham group, L 2 group and L 1 group than those in sham group and L 1 group. Compared with sham and L2 groups, the hippocampal CCPHP in SAH and L1 groups was higher than that in L2 group. Conclusion the same dose of lidocaine is more effective than intravenous injection in protecting the brain of rabbits with subarachnoid hemorrhage.
【作者单位】：贵州省人民医院ICU;贵州医科大学研究生院;
【基金】：贵州省科学技术基金(No.黔科合丁字[2007]2118)资助
【分类号】：R743.35

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