三苦滴丸抗实验性大鼠心肌缺血作用的炎症机制

发布时间：2018-05-14 17:32

本文选题：三苦滴丸 + 心肌缺血　；参考：《中国老年学杂志》2015年23期

【摘要】：目的观察三苦滴丸(SK)对盐酸异丙肾上腺素所致大鼠心肌缺血的保护作用相关炎性机制。方法将大鼠随机分为6组,空白组、模型组给予0.9%氯化钠注射液10 ml/kg,阳性对照组给予复方丹参滴丸405 mg/kg灌胃。SK高剂量组予SK 1 600 mg/kg灌胃,SK中剂量组予SK 800 mg/kg灌胃,SK低剂量组予SK 400 mg/kg灌胃,共给药10 d。连续3 d腹腔注射盐酸异丙肾上腺素5 mg/kg。第8、9天给予腹腔注射盐酸异丙肾上腺素(5 mg/kg),空白组给予等容量生理盐水。于第10天灌药后30 min,各组大鼠分别以10%乌拉坦(0.5 ml/100 g体重)麻醉,仰位固定,腹腔注射盐酸异丙肾上腺素(5 mg/kg),空白组给予等容量的生理盐水。造模成功后2 h腹主动脉采血,检测白细胞介素(IL)-1、IL-6、肿瘤坏死因子(TNF)α、血管细胞黏附因子(VCAM)-1;处死大鼠,取心脏,处理后行显微镜观察;观察SK对心肌细胞中1κB蛋白、NF-κBp65蛋白表达、NF-κB活性、NF-κBp65 mRNA基因表达的影响。结果不同剂量SK可不同程度减轻心肌缺血的程度,阻断心肌缺血炎症机制。结论 SK对盐酸异丙肾上腺素所致大鼠心肌缺血有良好的保护作用,可能与相关炎性机制有关。
[Abstract]:Objective to observe the protective effect of Sangui drop pill (SKK) on myocardial ischemia induced by isoprenaline hydrochloride in rats. Methods the rats were randomly divided into 6 groups, blank group, The model group was given 0.9% sodium chloride injection 10 ml / kg, and the positive control group was given the high dose of SK at 405 mg/kg in the positive control group. The middle dose group of SK 1 600 mg/kg was given SK 800 mg/kg intragastric perfusion of SK low dose group, and the low dose group was given SK 400 mg/kg for 10 days. 5 mg / kg isoprenaline hydrochloride was injected intraperitoneally for 3 days. Isoprenaline hydrochloride was injected intraperitoneally on the 8th day, 5 mg / kg of isoprenaline hydrochloride, and the blank group was given the same volume of normal saline. On the 10th day, the rats in each group were anesthetized with 10% uratan (0.5 ml/100 / g body weight), fixed in the supine position, intraperitoneally injected with isoproterenol hydrochloride (5 mg / kg), and the blank group was given the same volume of normal saline. Blood samples were collected from abdominal aorta 2 hours after the model. IL-6, TNFa and VCAM-1 were detected. The rats were killed, heart was taken and observed under microscope. To observe the effect of SK on the expression of NF- 魏 B and NF- 魏 Bp65 mRNA in cardiac myocytes. Results different doses of SK could attenuate the degree of myocardial ischemia and block the inflammatory mechanism of myocardial ischemia. Conclusion SK has a good protective effect on myocardial ischemia induced by isoproterenol in rats, which may be related to the inflammatory mechanism.
【作者单位】：长春中医药大学附属医院肾病科;吉林省卫生计生委;
【基金】：国家科技重大专项资助课题(No.2009ZX09102-115)
【分类号】：R285.5

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