钙激活氯通道TMEM16A在大鼠心房和心室中的表达差异

发布时间：2018-05-17 19:23

本文选题：TMEMA + 钙激活氯通道　；参考：《中国全科医学》2016年36期

【摘要】：目的探究钙激活氯通道(Ca CCs)TMEM16A在大鼠心房和心室组织中的表达情况,为临床治疗心肌缺血、心律失常以及心力衰竭等疾病提供新的治疗靶点。方法 2014年10月—2015年9月,将18只清洁级SpragueDawley大鼠适应性饲养1周,采用10%水合氯醛溶液腹腔注射麻醉后取心脏,分离心房和心室组织进行实验。采用Western blotting法和实时荧光定量反转录PCR(RT-q PCR)法分别检测大鼠心房和心室组织中TMEM16A及其mRNA水平。结果大鼠心房和心室组织TMEM16A水平分别为(0.46±0.02)和(0.33±0.03),心房和心室组织TMEM16A mRNA水平分别为(3.55±0.17)和(1.00±0.03),大鼠心房组织中TMEM16A及其mRNA水平高于心室组织(P0.01)。结论 Ca CCs TMEM16A在大鼠心房和心室组织中均有表达,其在心房组织中的水平高于心室组织。
[Abstract]:Objective to investigate the expression of Ca CCs)TMEM16A in rat atrium and ventricle, and to provide a new therapeutic target for the treatment of myocardial ischemia, arrhythmia and heart failure. Methods from October 2014 to September 2015, 18 clean grade SpragueDawley rats were fed adaptively for one week. The hearts were taken after intraperitoneal injection of 10% chloral hydrate solution to separate the atrial and ventricular tissues. The levels of TMEM16A and mRNA in rat atrium and ventricle were detected by Western blotting and real-time fluorescence quantitative reverse transcription (PCR(RT-q). Results the levels of TMEM16A in rat atrium and ventricle were 0.46 卤0.02) and 0.33 卤0.03m, respectively. The levels of TMEM16A mRNA in atrial and ventricular tissues were 3.55 卤0.17) and 1.00 卤0.03, respectively. The levels of TMEM16A and mRNA in rat atrial tissue were higher than those in ventricular tissue (P 0.01). Conclusion Ca CCs TMEM16A is expressed in rat atrium and ventricle, and the level of Ca CCs TMEM16A in atrial tissue is higher than that in ventricular tissue.
【作者单位】：石河子大学医学院生理学教研室新疆地方与民族高发病教育部重点实验室;石河子大学医学院第一附属医院;
【基金】：国家重点基础研究发展计划项目(973计划项目)(2012CB26600)
【分类号】：R331

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