铁过量补充对仔鼠神经反射及学习记忆能力影响的实验研究

发布时间：2018-05-18 04:07

本文选题：铁过量 + 仔鼠　；参考：《营养学报》2015年02期

【摘要】：目的研究孕期及哺乳期母鼠铁过量补充对仔鼠神经反射及学习记忆能力的影响。方法将受孕第0日孕鼠随机分为正常对照组(对照组)、低剂量铁补充组(低铁组)和高剂量铁补充组(高铁组)三组,每组10只,单笼饲养,自由进食全价颗粒(含铁量为370 mg/kg),饮用去离子水。自妊娠第l日开始对孕鼠进行铁干预,腹腔注射右旋糖酐铁,每2日1次,直至仔鼠断乳。对照组隔天腹腔注射生理盐水2 ml,低铁组、高铁组分别隔天腹腔注射30、120 mg/kg·bw右旋糖酐铁,注射前用生理盐水将每只孕鼠所需右旋糖酐铁注射液稀释为2 ml。连续5w每周称量仔鼠体质量,应用比色法测量仔鼠血清铁含量;应用悬崖回避、平面翻正、负趋地性试验测定仔鼠的神经反射功能;应用Morris水迷宫试验测定仔鼠学习记忆能力。5w后对仔鼠进行麻醉,断头取血,静置30 min,3 000 r/min离心10 min,取上清,于-20℃冻存。结果高铁组血清铁含量为61.87μmol/L,显著高于对照组的38.34μmol/L和低铁组的38.45μmol/L(P0.05)。神经反射试验结果显示,负趋地性试验中高铁组仔鼠的达标日龄为8.60 d,显著高于对照组的6.40 d和低铁组的5.40 d(P均0.05),低铁组与对照组相比差异无统计学意义(P0.05);悬崖回避试验中,高铁组仔鼠的达标为日龄6.90 d,显著高于对照组的3.60 d和低铁组的3.30 d(P均0.05),低铁组与对照组相比差异无统计学意义(P0.05);平面翻正试验中,高铁组仔鼠达标日龄为6.10 d,显著高于对照组的3.90 d和低铁组的2.70 d(P均0.05),且低铁组显著低于对照组(P0.05)。Morris水迷宫实验中,训练的第2日,高铁组仔鼠平均逃避潜伏期为37.80 s,显著长于对照组的26.85 s(P0.05),但与低铁组的31.93 s相比差异无统计学意义(P0.05)。训练的第5日,高铁组仔鼠平均逃避潜伏期为23.76 s,显著长于对照组的19.73 s和低铁组的15.77 s(P均0.05),两日中低铁组与对照组相比差异均无统计学意义(P均0.05)。结论孕期及哺乳期母鼠过量补充可损伤仔鼠神经反射功能,并对仔鼠的学习记忆能力可能产生不良影响。
[Abstract]:Objective to study the effect of iron excess supplementation on the neural reflex and learning and memory ability of offspring rats during pregnancy and lactation. Methods zeroth pregnant rats were randomly divided into normal control group (control group), three groups of low dose iron supplementation group (low iron group) and high dose iron supplement group (Gao Tiezu), 10 rats in each group, and free feeding of full price granules. The iron content was 370 mg/kg), drinking deionized water. From the first day of pregnancy, the pregnant rats were treated with iron intervention and intraperitoneal injection of iron dextran, 1 times every 2 days until the offspring were weaned. The control group was intraperitoneally injected with saline 2 ml, low iron group, and the high iron group was intraperitoneally injected with 30120 mg/ kg. BW dextran iron in the abdominal cavity every other day. The Iron Dextran Injection of pregnant rats was diluted to 2 ml. to weigh the body mass per week of 5W mice. The serum iron content was measured by colorimetric method. The nerve reflex function of the offspring was measured by the cliff avoidance, plane reversal and negative chemotactic test, and the Morris water maze test was used to determine the learning and memory ability of the offspring to be anaesthetized by the offspring of the offspring. Blood, 30 min, 3000 r/min centrifugation 10 min, Torikami Kiyo, and cryopreserved at -20 C. The serum iron content of the high iron group was 61.87 mu mol/L, which was significantly higher than that of the control group of 38.34 micron mol/L and the low iron group. The results of neural reflex test showed that the age of the mice in the negative chemotactic test was 8.60 D, significantly higher than that of the high iron group. The 6.40 D in the control group and 5.40 D in the low iron group (P 0.05), there was no significant difference between the low iron group and the control group (P0.05). In the cliff avoidance test, the standard of the high iron group was 6.90 D, significantly higher than the 3.60 D in the control group and 3.30 D in the low iron group (P all 0.05), and the low iron group had no statistical difference (P0.05) compared with the control group (P0.05). In the positive test, the age of the mice in the high iron group was 6.10 D, which was significantly higher than the 3.90 D in the control group and 2.70 D in the low iron group (P 0.05), and the low iron group was significantly lower than the control group (P0.05).Morris water maze experiment. The average escape latency of the high speed iron group was 37.80 s, which was significantly longer than the control group of 26.85 s (P0.05), but it was compared with the low iron group. The difference was not statistically significant (P0.05) compared with the 31.93 s. The average escape latency of the high speed iron group was 23.76 s, which was significantly longer than 19.73 s in the control group and 15.77 s in the low iron group (P 0.05). There was no significant difference between the middle and low iron groups (P 0.05) on two days (P 0.05). Conclusion excessive supplementation in pregnant and lactation rats could be impaired. The nerve reflex function of injured offspring rats may have adverse effects on learning and memory ability of offspring rats.
【作者单位】：青岛大学医学院公共卫生学院;
【基金】：国家自然科学基金(No.81373000)
【分类号】：R153.1

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