高海拔地区家兔挤压综合征模型的初步建立

发布时间：2018-05-20 06:38

本文选题：高原 + 挤压综合征　；参考：《青海大学》2017年硕士论文

【摘要】：目的:建立一种适合高海拔地区的、简单有效并且可复制的家兔挤压综合征(crush syndrome,CS)实验模型,为在高原地区进一步研究CS打下基础。材料与方法:健康成年新西兰家兔50只(饲养和实验地为西宁,海拔2260m)。随机分为1组对照组及9个实验组,按照挤压重量(20kg、30kg、40kg)和挤压时间(4h、6h、8h)的不同进行排列组合后形成9种不同的挤压条件,实验组于解除挤压后6h取静脉血检测血BUN、Cr、AST、ALT、CK、K+,解除压迫后24小时取家兔心肌、肾脏、肝脏、挤压处大腿四头肌行病理检查。对照组5只家兔作为空白对照,仅作麻醉处理不做挤压,在相同的时间点上取血及组织标本。结果:所有实验组家兔双后肢出现了明显的肿胀(P0.05);所有实验组家兔在解压后6h检测血BUN、Cr、AST、ALT、CK变化与对照组相比较均有统计学意义(p0.05),而且随着压迫重量和压迫时间的增加,各数值也随之增加;受压肌肉及肝肾组织活检提示均有显著的病理改变;但是随着压迫重量和时间的增加,实验组家兔死亡率也随之升高。结论:随着挤压重量和挤压时间的增加可加重挤压综合征的严重程度,在高海拔地区,为了保证较低死亡率可采取挤压双后肢、挤压重量为20Kg、挤压时间为4h可作为高海拔地区挤压综合征家兔模型的实验条件。
[Abstract]:Objective: to establish a simple, effective and reproducible rabbit model of crush syndrome (CSS) in high altitude, and to lay a foundation for further study of CS in plateau area. Materials and methods: 50 healthy adult New Zealand rabbits (Xining, raised and experimented at 2260 m above sea level). The control group and 9 experimental groups were randomly divided into two groups. According to the extrusion weight of 20 kg / L 30 kg / L 40 kg / L and the extrusion time of 4 h / 6 h / 8 h), 9 different extrusion conditions were formed in the experimental group. The rabbit myocardium, kidney, liver and thigh quadriceps were taken for pathological examination 24 hours after decompression. In the control group, 5 rabbits were used as blank control group. The blood and tissue samples were collected at the same time point. Results: there was significant swelling in both hind limbs in all the experimental groups, and the changes of blood BUNA CrASTASTTU CK in all the experimental groups were significantly different from those in the control group at 6 h after decompression, and with the increase of the compression weight and compression time, there was a significant difference between the experimental group and the control group. With the increase of compression weight and time, the death rate of rabbits in the experimental group also increased with the increase of compression weight and time. Conclusion: with the increase of extrusion weight and extrusion time, the severity of squeeze syndrome can be aggravated. In order to ensure lower mortality rate, compression of both hind limbs can be taken at high altitude. Extrusion weight of 20 kg and extrusion time of 4 h could be used as experimental conditions for rabbit model of extrusion syndrome at high altitude.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R642;R-332

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