虾青素对奥硝唑致雄性大鼠生殖损伤的保护作用及机制研究

发布时间：2018-05-20 14:35

本文选题：虾青素 + 精子　；参考：《南方医科大学》2017年硕士论文

【摘要】：目的:观察虾青素(AST)对奥硝唑(ORN)致雄性大鼠生殖功能损伤的保护作用,并探讨其初步作用机制,为其临床治疗不育提供理论依据。方法:取40只成年雄性SD大鼠,随机分成5组,每组8只,分别为:A组,0.5%羧甲基纤维素钠溶液+1 ml玉米油;B组,ORN 400 mg/(kg · d);C组,ORN 800 mg/(kg · d);D 组,ORN 400 mg/(kg · d)+ AST 20 mg/(kg · d);E 组,ORN 800 mg/(kg · d)+ AST 20 mg/(kg · d),灌胃。实验期间记录大鼠一般情况,3周后水合氯醛腹腔麻醉,剥离大鼠睾丸、附睾、精囊腺组织并称重,取一侧附睾尾部检测精子浓度和活力,剩余部分和一侧睾丸组织做病理切片观察;另一侧附睾组织匀浆,检测其GSH-Px、GR、CAT、SOD活性及MDA含量;另一侧睾丸组织匀浆后做组织8-OHdG含量和LDH、SDH、γ-GT活性检测。结果:1.实验期间A组大鼠活动反应正常,体重增加明显,皮毛有光泽。B、C组两组大鼠逐渐出现精神萎靡、反应变慢、嗜睡等多种症状,且随给药时间增加而加重。D、E组大鼠给予AST治疗后症状均得到改善。2.大鼠睾丸附睾HE结果显示,A组睾丸生精小管管腔内各级生精细胞排列紧密整齐,结构清晰,核染色致密均匀;附睾管管腔内见大量正常精子,纤毛排列整齐紧密。B、D组大鼠的睾丸和附睾组织形态与A组相比基本无差异。C组大鼠睾丸生精小管内的生精细胞明显减少,层次不明,细胞间隙变大,空泡增多;附睾管管腔内精子数量明显减少、排列疏松紊乱,可见多种非精子细胞成分和大片空白无染区域。E组大鼠睾丸组织结构有所恢复,附睾管内精子数量有所增加,但仍与A组有一定差距。3.与A组相比,B组的附睾尾精子活力,附睾GSH-Px、SOD活性显著降低,MDA含量显著上升(P0.05),其他指标改变不明显;C组的附睾尾精子活力、精子浓度、睾丸系数,附睾组织GSH-Px、GR、CAT、SOD活性和睾丸组织LDH、SDH、γ-GT活性均显著降低,而附睾MDA和睾丸8-OHdG含量显著上升(P0.05)。AST治疗后:与B组相比,D组附睾尾精子活力和附睾组织SOD活性显著增加,而MDA含量显著下降(P0.05);与C组相比,E组实验前后体重增量、精子活力、睾丸LDH、SDH活性显著增加,而MDA含量显著下降(P0.05),其他指标有所好转但无统计学差异。结论:AST可提高ORN所致的生殖损伤大鼠的精子质量,尤其是精子活力,其机制可能与提高大鼠附睾组织的抗氧化能力和睾丸标志酶的活性及抑制细胞NDA损伤有关。
[Abstract]:Objective: to observe the protective effect of astaxanthin (AST) on reproductive function injury induced by ornidazole (ORN) in male rats, and to explore its primary mechanism, and to provide theoretical basis for clinical treatment of sterility. Methods: forty adult male SD rats were randomly divided into 5 groups with 8 rats in each group. The rats in group B were treated with 0.5% sodium carboxymethylcellulose solution of 0.5% sodium carboxymethyl cellulose. Group B was given oral administration of ORN400 mg/(kg DX, group C, ORN800 mg/(kg DX, group D, ORN400 mg/(kg, AST 20 mg/(kg, group E, AST 20 mg/(kg d, AST 20 mg/(kg dl, respectively. During the experiment, the rats were anesthetized with chloral hydrate for 3 weeks, the testis, epididymis and seminal vesicle were removed and weighed. The sperm concentration and motility were measured by the tail of one epididymis. The rest of testis and one side of testis were observed by pathological section, the other side of epididymis homogenate was examined for the activity of SOD and MDA in GRCATU, and the content of 8-OHdG and activity of 8-OHdG and 纬 -GT in homogenate of the other side of testis were detected. The result is 1: 1. During the experiment, the rats in group A had normal activity reaction, weight gain was obvious, and the rats of group C with lustrous fur gradually developed many symptoms, such as mental retardation, slow reaction, drowsiness and so on. With the increase of administration time, the symptoms of rats in group .DnE were improved after AST treatment. The results of HE showed that the spermatogenic cells in the seminiferous tubule of the testis in group A were arranged neatly, the structure was clear, the nucleus staining was dense and uniform, and a large number of normal spermatozoa were found in the cavities of the epididymis. The morphology of testis and epididymis in group D was similar to that in group A. the spermatogenic cells in testicular seminiferous tubules of group C were obviously decreased, the level of spermatogenic tubules was not clear, the intercellular space became larger and the vacuoles increased. The number of spermatozoa in the epididymal duct was decreased obviously and the arrangement was loose. It was found that the structure of testis in group E was recovered and the number of spermatozoa in epididymal duct was increased. However, there was still a certain gap between group A and group A. Compared with group A, the sperm motility of cauda epididymidis in group B and the activity of GSH-PxX SOD in epididymal cauda significantly decreased the content of MDA increased significantly (P 0.05). The other indexes did not change significantly in group C, such as sperm motility, sperm concentration and testis coefficient. The activity of SOD in epididymal tissue and the activity of LDH- SDH, 纬 -GT in testicular tissue decreased significantly, while the contents of MDA and 8-OHdG in testis increased significantly after treatment with P0.05. AST: compared with group B, the sperm motility of caudal epididymis and the activity of SOD in epididymal tissue in group D were significantly increased. Compared with group C, the weight increment, sperm motility and the activity of MDA in testis increased significantly in group E, while the content of MDA decreased significantly (P 0.05), but there was no statistical difference between group E and group C. Conclusion the effects of ORN on sperm quality, especially sperm motility, may be related to the increase of antioxidant capacity and testicular marker enzyme activity of rat epididymis and the inhibition of NDA damage.
【学位授予单位】：南方医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R698.2

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