银杏叶提取物对肠缺血再灌注肝损伤AhR表达的影响

发布时间：2018-05-23 10:12

本文选题：银杏叶提取物 + 缺血再灌注损伤　；参考：《湖北科技学院》2016年硕士论文

【摘要】：目的:建立大鼠肠缺血再灌注损伤的模型,观察银杏叶提取物对大鼠肠缺血再灌注肝损伤的保护作用,并初步探讨银杏叶提取物对芳香烃受体(Ah R)表达的影响。方法:选择32只健康雄性SD大鼠,随机分为四个组。分别为正常组、模型组、EGb小剂量组、EGb大剂量组。正常组的大鼠被麻醉后直接开腹取静脉血和肝组织;模型组麻醉后开腹分离肠系膜上动脉,用动脉夹夹闭肠系膜上动脉根部30分钟,然后开放60分钟,形成缺血再灌注的模型;EGb给药组阴茎背静脉注射舒血宁注射液1小时后再制备缺血再灌注损伤的模型。采集标本后做如下检查:1.检测各组血清中的丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST);2.光镜下观察肝组织形态学变化;3.生化法检查肝组织中芳香烃受体(Ah R)和丙二醛(MDA)的变化;4.western-blot法检测肝组织中Ah R的表达量;5.实时荧光定量PCR检测肝组织Ah R的表达量。结果:(1)与正常组比,模型组血清中ALT和AST显著升高;与模型组比,EGb大剂量组中ALT和AST显著下降,均有统计学意义。(2)肝脏病理变化:与正常组比,模型组的肝组织排列没有规律,中央静脉和肝血窦淤血严重,局部坏死;与模型组,小剂量给药组淤血稍微减轻,其他没有明显的变化,大剂量给药组淤血情况明显减轻,炎症细胞浸润减少。(3)生化法检测肝组织MDA:与正常组比,模型组MDA含量明显升高;与模型组比,EGb大剂量组MDA显著减少,都有统计学意义。(4)Western blot法检测大鼠肝组织Ah R:与正常组比,模型组Ah R的含量显著升高;与模型组比,EGb大剂量组能明显降低Ah R含量,且都有统计学意义。(5)实时荧光定量检测Ah R的表达量:与正常组比,模型组Ah R表达量显著增加;与模型组比,EGb大剂量组Ah R的表达显著减少,均有统计学意义。结论:肠缺血再灌注可以导致小肠和肝脏的损伤,银杏叶提取物预处理可以明显降低Ah R的表达量,对大鼠肠缺血再灌注肝损伤有保护作用。
[Abstract]:Aim: to establish a rat model of intestinal ischemia-reperfusion injury, to observe the protective effect of Ginkgo biloba extract on intestinal ischemia-reperfusion liver injury in rats, and to explore the effect of Ginkgo biloba extract on the expression of aromatics receptor (Ah R). Methods: 32 healthy male SD rats were randomly divided into four groups. They were normal group, model group, low dose group and high dose group. In the normal group, the venous blood and liver tissue were taken directly after anesthesia, and the superior mesenteric artery was separated from the superior mesenteric artery after anesthesia, and the root of the superior mesenteric artery was clamped for 30 minutes and then opened for 60 minutes. The model of ischemia-reperfusion injury was established in the EGB group after 1 hour of injection of Shuxuening injection into the dorsal vein of penis to establish the model of ischemia-reperfusion injury. The specimens were collected and examined as follows: 1. Serum alanine aminotransferase (alt) and aspartate aminotransferase (AST) were detected. The morphologic changes of liver tissue were observed under light microscope. The changes of aromatics receptor (Ah R) and malondialdehyde (MDAA) in liver tissue were detected by biochemical method. 4. Western blot method was used to detect the expression of Ah R in liver tissue. The expression of Ah R in liver tissue was detected by real-time fluorescence quantitative PCR. Results compared with the normal group, the serum ALT and AST in the model group were significantly higher than those in the model group, and the ALT and AST levels in the model group were significantly lower than those in the model group (P < 0.01). The liver pathological changes of the model group were statistically significant: compared with the normal group, the liver tissue arrangement of the model group was not regular. The central vein and hepatic sinusoids were seriously congested and local necrosis was observed. Compared with the model group, the low dose drug administration group reduced the congestion slightly, but there was no obvious change in the others, while the large dose administration group obviously alleviated the congestion. Compared with the normal group, the content of MDA in the model group was significantly higher than that in the model group, and the MDA in the large dose group was significantly lower than that in the model group. Compared with the model group, the content of Ah R in the model group was significantly increased, and the expression of Ah R in the model group was significantly decreased compared with the model group, and there was statistically significant difference between the high dose group and the model group, and there was a significant increase in the expression of Ah R in the model group compared with the normal group. Compared with the model group, the expression of Ah R in the high dose of EGB group was significantly decreased, and there was significant difference between the two groups. Conclusion: intestinal ischemia-reperfusion can lead to the injury of small intestine and liver. Ginkgo biloba extract pretreatment can obviously reduce the expression of Ah R, and has protective effect on intestinal ischemia-reperfusion liver injury in rats.
【学位授予单位】：湖北科技学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R285.5

【相似文献】