针刺结合关元灸对CRS上皮炎症的影响及其机制初探

发布时间：2018-05-24 19:01

本文选题：针灸 + 慢性鼻窦炎　；参考：《成都中医药大学》2014年硕士论文

【摘要】：目的：观察针刺结合关元灸对小鼠慢性鼻窦炎(CRS)模型鼻窦黏膜中NF-kBp50蛋白及IFN-γ表达水平的影响,探索针刺结合关元灸治疗CRS的可能机制以及慢性鼻窦炎发生发展的分子生物学机制。方法：选用C57BL/6J小鼠160只,适应性喂养一周后,按入室顺序先后编号,采用DPS软件将160小鼠随机分为：正常组,模型组,假手术组,针灸组,西药组,中药常规剂量组,中药低剂量组,中药高剂量组,共8组,每组20只(本论文只纳入针灸组、西药组、假手术组、正常组、模型组5组数据)。随后进行小鼠慢性鼻窦炎模型建立。造模后第1天进行观察,造模后第8周开始,针灸组给予针刺足三里与灸关元交替治疗,西药组给予克拉霉素治疗。治疗14天后,将小鼠麻醉后处死,在超净冰面台上取出鼻黏膜组织。在光镜下观察各组鼻黏膜的病理形态变化。以免疫组化SABC法测定NF-kBp50蛋白表达及丫-干扰素(IFN-γ)表达水平。结果：1、症状及体征：模型组的鼻塞、流涕、喷嚏、搔抓鼻部症状仍然存在,并伴有溢泪及结膜充血水肿症状。针灸组、西药组喷嚏、流涕症状有所改善,喷嚏及抓鼻次数较少,无溢泪及结膜充血水肿表现。2、病理HE切片示：模型组鼻黏膜呈慢性炎症表现,黏膜炎细胞浸润,上皮细胞明显增生,纤毛及上皮细胞排列紊乱,部分区域变性、坏死、脱落,形成多个小溃疡。针灸组鼻黏膜上皮修复良好,炎细胞浸润、腺体及杯状细胞增生情况不明显。3、模型组NF-κ Bp50蛋白在鼻黏膜中表达的平均光密度值增大,相比正常组及假手术组有显著性差异(P0.01)；治疗后,针灸组、西药组NF-κ Bp50蛋白在鼻黏膜中表达的平均光密度值减小,较模型组有显著性差异(P0.01)；2、模型组IFN-γ在鼻黏膜中表达水平相比正常组及假手术组无差异(P0.05)。治疗后,针灸组、西药组IFN-γ在鼻黏膜中表达的平均光密度值增大,较模型组有差异(P0.05)。结论：针刺结合关元灸能够有效改善慢性鼻窦炎模型小鼠的全身症状,降低慢性鼻窦炎小鼠鼻黏膜中NF-κBp50蛋白的表达水平,促进IFN-γ的表达,从而抑制多种炎性因子的产生,纠正Th1/Th2的失衡状态,提高慢性鼻窦炎模型小鼠的机体免疫能力,减轻鼻黏膜的炎症反应,对炎性反应导致的鼻-鼻黏膜损伤有一定修复作用；
[Abstract]:Objective: to observe the effect of acupuncture combined with Guanyuan moxibustion on the expression of NF-kBp50 protein and IFN- 纬 in sinusoidal mucosa of mice with chronic sinusitis. To explore the possible mechanism of acupuncture combined with Guanyuan moxibustion in the treatment of CRS and the molecular biological mechanism of the occurrence and development of chronic sinusitis. Methods: 160 C57BL/6J mice were randomly divided into normal group, model group, sham operation group, acupuncture group, western medicine group and traditional Chinese medicine group. There were 20 rats in each group in the low dose group and high dose group of traditional Chinese medicine (this paper only included acupuncture group, western medicine group, sham-operation group, normal group and model group). Then the model of chronic sinusitis in mice was established. The model was observed on the first day after the model was made. The acupuncture group was treated with acupuncture Zusanli alternately with moxibustion Guanyuan and the western medicine group was treated with clarithromycin from the 8th week after the model making. After 14 days of treatment, the mice were anesthetized and killed, and nasal mucosa was removed from the super-clean ice table. The pathological changes of nasal mucosa in each group were observed under light microscope. The expression of NF-kBp50 and IFN- 纬 were detected by immunohistochemical SABC method. Results 1, symptoms and signs: nasal congestion, runny, sneezing, scratching and scratching nose were still present in the model group, accompanied by tears and conjunctival hyperemia and edema. In acupuncture group and western medicine group, the symptoms of sneezing and runny were improved, the number of sneezing and scratching nose was less, there were no tears and conjunctival hyperemia edema. The pathological HE section showed that the nasal mucosa of model group showed chronic inflammation and mucositis cell infiltration. Epithelial cells proliferated, cilia and epithelial cells were arranged in disorder, some areas were denatured, necrotized, shed, and many small ulcers were formed. In the acupuncture and moxibustion group, the nasal mucosal epithelium was repaired well, inflammatory cells infiltrated, glandular and goblet cell hyperplasia was not obvious. The average optical density of NF- 魏 Bp50 protein expression in the nasal mucosa in the model group was increased. After treatment, the average optical density of NF- 魏 Bp50 protein expression in nasal mucosa of acupuncture group and western medicine group decreased. Compared with the model group, there was a significant difference between the model group and the model group (P 0.01). The expression level of IFN- 纬 in the nasal mucosa of the model group was not different from that of the normal group and the sham operation group (P 0.05). After treatment, the mean optical density of IFN- 纬 expression in nasal mucosa of acupuncture group and western medicine group was increased, which was different from that of model group (P 0.05). Conclusion: acupuncture combined with Guanyuan moxibustion can effectively improve the systemic symptoms of mice with chronic sinusitis, decrease the expression of NF- 魏 Bp50 protein in nasal mucosa of chronic sinusitis mice, promote the expression of IFN- 纬, and thus inhibit the production of various inflammatory factors. To correct the imbalance of Th1/Th2, to improve the immune ability of mice with chronic sinusitis, to alleviate the inflammatory reaction of nasal mucosa, and to repair the rhino-nasal mucosal injury caused by inflammatory reaction.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R246.81

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