螺旋藻激酶对内皮损伤大鼠纤溶酶原和抗凝血酶Ⅲ的影响

发布时间：2018-05-25 09:07

本文选题：螺旋藻激酶(SPK) + 纤溶酶原(PLG)　；参考：《中国老年学杂志》2017年12期

【摘要】：目的研究螺旋藻激酶(SPK)对活体内皮损伤大鼠血管内皮细胞抗凝和溶栓相关因子的影响,探讨SPK对心血管系统的保护作用。方法建立体内大鼠内皮损伤血栓模型。将大鼠随机分为正常对照组、损伤模型组、SPK低、中、高剂量组。不同剂量SPK干预后麻醉动物,Fe Cl3诱导大鼠颈总动脉内皮损伤形成血栓,从大鼠腹主动脉取血,抗凝后用酶联免疫吸附法(ELISA)检测纤溶酶原(PLG)和抗凝血酶(AT)-Ⅲ的含量。结果动物模型构建成功后损伤模型组与正常对照组相比,大鼠血液中PLG和AT-Ⅲ的含量显著升高(P0.01),SPK各剂量组PLG和AT-Ⅲ含量明显升高(P0.01),在中、高剂量组最为显著。结论 SPK在抗凝溶栓效应上,促进PLG和AT-Ⅲ释放,提高内皮细胞抗凝溶栓酶活性,从而发挥抗血栓作用。
[Abstract]:Objective to study the effect of Spirulina kinase (SPK) on the anticoagulant and thrombolytic related factors of vascular endothelial cells in rats with living endothelial injury and to explore the protective effect of SPK on cardiovascular system. Methods the rat model of endothelium injury thrombus was established in vivo. The rats were randomly divided into normal control group, damage model group, low SPK, high dose group and different dose of SPK. After the intervention of the anesthetized animals, Fe Cl3 induced the endothelial injury of the common carotid artery to form a thrombus, the blood was taken from the abdominal aorta of the rat, and the content of the plasminogen (PLG) and the antithrombin (AT) - III were detected by the enzyme linked immunosorbent assay (ELISA) after anticoagulation. The results of the animal model group were compared with the normal control group, and the PLG and AT- in the rat blood were compared with the normal control group. The content of III was significantly increased (P0.01), and the content of PLG and AT- III in each dose group of SPK increased significantly (P0.01). In the middle, the high dose group was the most significant. Conclusion SPK in anticoagulant thrombolytic effect, promote the release of PLG and AT- III, improve the activity of anticoagulant thrombolytic enzyme in endothelial cells, and thus play an anti thrombolytic effect.
【作者单位】：广西医科大学附属肿瘤医院;广西医科大学基础医学院;清华大学化工系;
【基金】：广西壮族自治区自然科学基金(No.2013GXNSFAA019176)
【分类号】：R965

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