离心运动诱导大鼠骨骼肌损伤中丝氨酸蛋白酶Omi的表达升高

发布时间：2018-05-26 09:56

本文选题：离心运动 + 骨骼肌　；参考：《天津体育学院学报》2015年02期

【摘要】：目的:旨在观察离心运动致骨骼肌损伤后丝氨酸蛋白酶(Omi)在骨骼肌组织中的表达变化。方法:8周龄雄性SD大鼠36只,随机分为安静对照(n=6)、离心运动2大组(n=30),离心运动组大鼠在跑台上进行一次性离心运动,分别于运动后即刻(E0组,n=6)、运动后12 h(E12组,n=6)、运动后24 h(E24组,n=6)、运动后48 h(E48组,n=6)和运动后72 h(E72组,n=6)麻醉大鼠,腹主动脉取血,取比目鱼肌待测;使用荧光酶联免疫吸附法检测半胱氨酸天冬氨酸特异性蛋白酶-3(Caspase-3)的活性;Western Blot和Realtime-PCR法分别检测Omi、凋亡抑制蛋白(XIAP)的蛋白表达和基因水平;Co-IP法检测XIAP和Omi的相互结合作用。结果:与对照组相比,离心运动后Caspase-3、Caspase-9活性均明显升高;离心运动后Omi、XIAP的蛋白表达和m RNA水平均明显升高;离心运动能明显增加XIAP和Omi的结合量。结论:离心运动能够激活骨骼肌组织Caspase-9和Caspase-3的活性,诱导Omi和XIAP的表达上调,促进XIAP与Omi相互结合,Omi可能在促使凋亡过程中发挥重要介导作用。
[Abstract]:Aim: to observe the expression of serine proteinase (Omiase) in skeletal muscle after eccentric exercise. Methods Thirty-six male Sprague-Dawley rats (8 weeks old) were randomly divided into two groups: control group (n = 6), centrifugal exercise group (n = 2), and centrifugal exercise group (n = 30). The anesthetized rats in E0 group, 12 h(E12 group, 24 h(E24 group, 48 h(E48 group and 72 h(E72 group respectively were anesthetized immediately after exercise. The abdominal aorta blood was taken and the soleus muscle was taken. The activity of cysteine aspartate specific protease -3caspase-3 was detected by fluorescence enzyme-linked immunosorbent assay (FElisa). Western Blot and Realtime-PCR methods were used to detect the protein expression of Omiand XIAPs, respectively. The interaction between XIAP and Omi was detected by co-IP method at gene level. Results: compared with the control group, the activity of Caspase-3 and Caspase-9 was significantly increased after centrifugation, the protein expression and m RNA level of Omio XIAP were significantly increased after centrifugal exercise, and the binding amount of XIAP and Omi was significantly increased after centrifugal exercise. Conclusion: centrifugal exercise can activate the activity of Caspase-9 and Caspase-3 in skeletal muscle, induce the expression of Omi and XIAP up-regulate, and promote the interaction between XIAP and Omi, which may play an important role in the process of apoptosis.
【作者单位】：太原理工大学体育学院;成都体育学院期刊部;北京体育大学运动人体科学学院;
【基金】：国家自然科学基金项目(项目编号:31471133) 中央高校基本科研业务费专项资金资助课题(项目编号:2014SYS005) 太原理工大学人才基金资助项目(项目编号:tyut-rc201452a)
【分类号】：G804.2

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