多部位绝对不应期电刺激对慢性心力衰竭兔心功能的影响

发布时间：2018-05-27 02:35

本文选题：绝对不应期 + 电刺激　；参考：《中国老年学杂志》2015年17期

【摘要】：目的通过升主动脉套扎法建立兔压力负荷过重型慢性心力衰竭(CHF)模型,观察绝对不应期电刺激对兔CHF模型心功能的影响,同时比较多部位刺激是否较单部位刺激更为有效和安全。方法 30只健康新西兰大白兔通过升主动脉根部缩窄建立兔CHF模型,根据心脏收缩调节(CCM)电极放置部位随机分为单部位刺激组、双部位刺激组和三部位刺激组。三组均每天刺激6 h,连续刺激7 d。于刺激前后采用无创心脏超声(UCG)检测心功能、双抗体夹心ABC-ELISA法检测血浆脑钠肽(BNP)。结果 CCM刺激前三组超声心功能各项指标无明显差别(P0.05)。CCM刺激7 d以后,三组左心室收缩末期内径(LVESD)、左心室舒张末期内径(LVEDD)较刺激前均明显下降,三部位刺激组和双部位刺激组LVESD、LVEDD下降程度较单部位明显(P0.05),三部位刺激组与双部位刺激组下降程度未见明显差异(P0.05)。三组左室射血分数(LVEF)、左室短轴缩短率(LVFS)均明显升高,双部位及三部位刺激组升高较单部位更加明显(P0.05),双部位与三部位升高程度未见明显差异(P0.05)。CCM刺激前血浆BNP水平三组相比无明显差别(P0.05)。CCM刺激7 d后血浆BNP水平双部位刺激组、三部位刺激组降低程度较单部位刺激组明显(P0.05),三部位与双部位刺激组相比降低程度水平没有明显变化(P0.05)。结论绝对不应期电刺激可增加CHF心肌的收缩力,改善心功能;两部位刺激与三部位刺激较单部位刺激可更好地改善心功能,两部位同时刺激与三部位同时刺激在改善心功能方面未见明显差异。
[Abstract]:Objective to establish rabbit model of severe chronic heart failure with pressure overload by ascending aortic ligation, and to observe the effect of electrical stimulation of absolute refractory period on cardiac function of rabbit CHF model. At the same time, to compare whether multi-site stimulation is more effective and safe than single site stimulation. Methods CHF model was established in 30 healthy New Zealand rabbits by constricting the root of ascending aorta. According to the position of CCM electrode placed in cardiac contractile regulator, the rabbits were randomly divided into three groups: single site stimulation group, double site stimulation group and three-site stimulation group. All the three groups were stimulated for 6 hours per day for 7 days. Cardiac function was detected by noninvasive echocardiography before and after stimulation, and plasma brain natriuretic peptide (BNP) was detected by double antibody sandwich ABC-ELISA. Results after 7 days of CCM stimulation, the left ventricular end-systolic diameter (LVESD) and left ventricular end-diastolic diameter (LVEDDD) were significantly decreased in the three groups. The decrease of LVESDN LVEDD in the three site stimulation group and the double site stimulation group was significantly lower than that in the single site stimulation group (P 0.05), but there was no significant difference between the three site stimulation group and the double site stimulation group. Left ventricular ejection fraction (LVEF), left ventricular shortening rate (LVFS) and left ventricular shortening rate (LVFSs) were significantly increased in the three groups. There was no significant difference in the level of plasma BNP between the two parts and the three parts before stimulation. There was no significant difference in plasma BNP level between the three groups after 7 days of stimulation. The decrease of P0.05 in the three-site stimulation group was significantly higher than that in the single-site stimulation group, but there was no significant change in the decrease level of the three-site stimulation group compared with the double-site stimulation group. Conclusion Electrical stimulation of absolute refractory period can increase the contractility of CHF myocardium and improve cardiac function, and the stimulation of two parts and three parts can improve the cardiac function better than that of single part stimulation. There was no significant difference in the improvement of cardiac function between simultaneous stimulation of two sites and simultaneous stimulation of three parts.
【作者单位】：河北医科大学研究生学院;河北省人民医院心脏中心;
【基金】：河北省自然科学基金项目(C2010001631)
【分类号】：R541.6

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