不同规格毫针针刺对大鼠穴区筋膜组织、细胞和胶原纤维的影响

发布时间：2018-05-27 10:06

本文选题：毫针针刺 + 筋膜结缔组织　；参考：《中国针灸》2015年08期

【摘要】：目的:探讨不同规格毫针针刺对大鼠腧穴穴区筋膜结缔组织结构、细胞活性、胶原纤维排列形态及含量的影响。方法:将32只SD大鼠随机分为空白组、细针组、中针组和粗针组,每组8只。除空白组外,其他组分别采用不同规格毫针在大鼠"中脘"沿任脉循行方向平刺,施以捻转平补平泻刺激,每天1次,空白组予相同的麻醉、抓取、固定。干预3天后,切取各组穴区筋膜结缔组织,采用HE染色、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)免疫组化染色和MASSON染色,镜下观察腧穴筋膜结缔组织形态、细胞中PCNA表达、胶原纤维的排列形态和表达变化。结果:各组毫针针刺后,腧穴筋膜结缔组织形态及胶原纤维排布一致性发生改变;各组腧穴筋膜结缔组织细胞中PCNA蛋白表达均明显增强(P0.01,P0.05);胶原纤维的面积分布发生改变,其中细针组较空白组增大,但差异无统计学意义(P0.05),中针组与粗针组胶原纤维的面积分布均较空白组减小(均P0.05)。结论:不同规格毫针针刺均能够改变受治腧穴穴区局部组织形态、增强细胞活性和调节胶原纤维蛋白表达变化,这可能是毫针针刺治疗"通经脉"作用的细胞生物力学原理之一,但规格不同,其所产生的刺激量不同,穴区组织细胞和胶原纤维变化的量-效差异存在复杂关系。
[Abstract]:Objective: to investigate the effects of different kinds of millimeter acupuncture on connective tissue structure, cell activity, collagenous fiber arrangement and content of fascia in acupoints of rats. Methods: 32 Sprague-Dawley rats were randomly divided into three groups: blank group, fine needle group, middle needle group and coarse needle group, with 8 rats in each group. In addition to the blank group, the other groups were treated with the same anesthesia, grasping and fixing the same anesthesia as the blank group, respectively, by using different specifications of the needle in the direction of "Zhongwan" along the meridian of the rat, and applying twirling, tonifying, flattening and reducing stimulation once a day. After 3 days of intervention, the connective tissue of the fascia of the acupoints in each group was removed and stained with HE, proliferating cell nuclear antigen-PCNA (PCNA) and MASSON staining. The connective tissue morphology of acupoints and the expression of PCNA in the cells were observed under microscope. Changes in the morphology and expression of collagen fibers. Results: after acupuncture, the morphology of connective tissue and the arrangement consistency of collagenous fibers in acupoints were changed, the expression of PCNA protein in connective tissue cells of acupoints was significantly enhanced, and the area distribution of collagen fibers was changed. The area distribution of collagen fibers in the medium needle group and the coarse needle group were all decreased compared with the blank group (P 0.05), but there was no significant difference between the fine needle group and the blank group (P 0.05). Conclusion: acupuncture with different specifications can change the local tissue morphology, enhance cell activity and regulate the expression of collagen fibrin in acupoints. This may be one of the cellular biomechanical principles in the treatment of "Tongjing", but with different specifications and different stimuli, there is a complex relationship between the changes of tissue cells and collagen fibers in the acupoint area.
【作者单位】：贵阳中医学院针灸推拿学院;贵州省第二人民医院康复科;
【基金】：国家自然科学基金项目:81160456 贵州省高等学校特色重点实验室建设项目:黔教合KY字[2014]218号
【分类号】：R245

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