夏荔芪胶囊对良性前列腺增生模型大鼠PCNA、caspase-3表达水平的影响

发布时间：2018-05-27 12:01

本文选题：良性前列腺增生 + 夏荔芪胶囊　；参考：《中华男科学杂志》2017年08期

【摘要】：目的:探讨夏荔芪胶囊对良性前列腺增生(BPH)模型大鼠增殖细胞核抗原(PCNA)、半胱氨酸蛋白酶3(caspase-3)表达水平的影响。方法:50只雄性SD大鼠,随机分为5组,每组10只:空白组(假手术组),模型组,夏荔芪高、低剂量治疗组[1.20 g/(kg·d)、0.61 g/(kg·d)]及非那雄胺治疗组[0.8 mg/(kg·d)]。采用去势后皮下注射丙酸睾酮[0.5 mg/(kg·d),30 d],建立大鼠BPH模型。空白组及模型组予以生理盐水,治疗组予以相应药物,造模成功后连续灌胃30 d。实验结束后将各组大鼠于麻醉下留取双侧前列腺组织后处死,计算大鼠前列腺指数(前列腺湿重/体重);免疫组化法检测大鼠前列腺组织中PCNA的表达情况;免疫荧光法检测大鼠前列腺组织中caspase-3的表达水平。结果:与空白组相比,模型组的前列腺湿重(g)及前列腺指数(mg/g)明显增加(1 326±60 vs 471±17;2.89±0.18 vs 1.06±0.06,P均0.01);与模型组比较,各治疗组的前列腺湿重及前列腺指数则明显降低,其中,夏荔芪高剂量组较低剂量组下降更明显(914±36 vs 1 099±46;2.02±0.08 vs 2.39±0.11,P均0.01),而各治疗组中以非那雄胺组(817±53 vs 471±17;1.83±0.10 vs 1.06±0.06,P均0.01)降低最明显。免疫组化结果显示,与空白组相比,模型组大鼠前列腺组织中PCNA表达明显增多,各治疗组与模型组相比,前列腺组织中PCNA表达均有明显减少,其中夏荔芪高剂量治疗组减少较为明显。免疫荧光结果显示,与空白组相比,模型组大鼠前列腺组织caspase-3表达量降低,各治疗组与模型组相比,前列腺组织中caspase-3表达明显增高,其中非那雄胺治疗组增高较为明显。结论:夏荔芪胶囊能明显降低BPH大鼠前列腺湿重及前列腺指数,通过降低前列腺组织中PCNA的表达水平,增高前列腺组织中caspase-3的表达,可能是其治疗BPH大鼠的机制。
[Abstract]:Aim: to investigate the effect of Xialiqi capsule on the expression of proliferating cell nuclear antigen (PCNA) and cysteine protease 3 (caspase-3) in BPH model rats. Methods Fifty male Sprague-Dawley rats were randomly divided into 5 groups, 10 rats in each group: blank group (sham operation group, model group, Xialiqi high dose group, low dose group [1.20 g/(kg DX 0.61 g/(kg d] and fenasteride group [0.8 mg/(kg d]). Rat BPH model was established by subcutaneous injection of testosterone propionate [0.5 mg/(kg / d] after castration. The blank group and the model group were given normal saline, and the treatment group were given the corresponding drugs. After the experiment, the rats in each group were sacrificed after the bilateral prostate tissue was taken out under anesthesia, the prostate index (wet prostate weight / body weight) was calculated, and the expression of PCNA in the prostate tissue was detected by immunohistochemical method. The expression of caspase-3 in rat prostate was detected by immunofluorescence method. Results: compared with the blank group, the prostatic wet weight (g) and prostate index (mg / g) in the model group were significantly increased by 1 326 卤60 vs 471 卤172.89 卤0.18 vs 1.06 卤0.06 (P) 0.01, and the wet weight of prostate and the prostate index in each treatment group were significantly lower than those in the model group. In the high dose group, 914 卤36 vs 1 099 卤46 0. 02 卤0. 08 vs 2. 39 卤0. 11 (P < 0. 01), but in the fenasteride group (817 卤53 vs 471 卤17 卤1. 83 卤0. 10 vs 1. 06 卤0. 06 P 0. 01). The results of immunohistochemistry showed that compared with the blank group, the expression of PCNA in the prostate tissue of the model group was significantly increased, and the expression of PCNA in the prostate tissue of each treatment group was significantly decreased compared with the model group. The decrease of high dose treatment group was more obvious. The results of immunofluorescence showed that compared with the blank group, the expression of caspase-3 in the prostate tissue of the model group was lower than that of the control group, and the expression of caspase-3 in the prostate tissue of each treatment group was significantly higher than that in the model group, especially in the fenasteride group. Conclusion: Xialiqi capsule can obviously reduce the wet weight of prostate and the index of prostate in BPH rats. It may be the mechanism of the treatment of BPH rats by decreasing the expression of PCNA and increasing the expression of caspase-3 in prostate tissue.
【作者单位】：南方医科大学附属金陵医院/南京军区南京总医院男科;南京医科大学第一临床医学院;华中科技大学计划生育研究所/生殖医学中心;广西医科大学第四附属医院/柳州市工人医院泌尿外科;西安市第四医院生殖医学科;南京大学医学院附属金陵医院/南京军区南京总医院男科;
【分类号】：R285.5;R-332

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