粪肠球菌对大鼠根尖周炎TNF-α基因表达影响的研究

发布时间：2018-05-27 16:26

  本文选题：肿瘤坏死因子α + 根尖周炎　； 参考：《大连医科大学》2014年硕士论文

【摘要】：目的：研究粪肠球菌在大鼠根尖周炎过程中对肿瘤坏死因子α（TNF-α）的表达影响。 方法：选取健康雄性SD大鼠24只，右侧下颌第一磨牙开髓后，，随机分为A、B两组，每组各12只。A组在髓腔内封入PBS缓冲液棉球,设为未封菌组；B组在髓腔内封入粪肠球菌与PBS缓冲液调制的菌悬液棉球,设为封菌组。两组均以玻璃离子粘固剂封闭髓腔，调牙合；健康的左侧下颌第一磨牙为空白对照组。A、B两组分别于术后1周、2周、3周、4周四个时间点随机各取3只大鼠，多聚甲醛麻醉，去除软组织，分离下颌骨，用挖匙分别刮取右侧下颌第一磨牙和左侧下颌第一磨牙根尖周组织，提取组织RNA，逆转录成cDNA，采用实时荧光定量PCR(Real-timePCR)手段检测各组TNF-αmRNA的表达水平并进行统计学分析。 结果： 1.TNF-αmRNA在空白对照组有少量表达。A组和B组TNF-αmRNA表达量与空白对照组比均增高，差异具有统计学意义(p＜0.05)。 2.A组术后，1周到2周TNF-αmRNA表达量逐渐增高，2周达到最高，3周下降，4周继续下降。其中2周与3周相比，差异有统计学意义(p＜0.05)。 3.B组术后，1周到2周TNF-αmRNA表达量逐渐增高，到2周达到高峰，差异有统计学意义(p＜0.05)。2周到4周，TNF-αmRNA表达量呈下降趋势差异有统计学意义(p＜0.05)。 4.B组TNF-α mRNA表达量高于A组，差异有统计学意义(p＜0.05)。 结论： 1.TNF-α mRNA的表达水平与根尖周炎密切相关，提示TNF-α参与根尖周炎的炎症和破骨过程。 2.TNF-α与粪肠球菌感染为主的根尖周炎密切相关，提示粪肠球菌可能加重了根尖周组织的炎症反应和破骨。
[Abstract]:Objective: To study the effect of Enterococcus faecalis on expression of tumor necrosis factor alpha (TNF- alpha) in periapical periodontitis of rats.
Methods: 24 healthy male SD rats were selected, and the right mandibular first molar was randomly divided into A and B two groups. 12.A groups each in each group were sealed into the PBS buffer solution cotton ball in the medullary cavity. The B group was sealed in the medullary cavity with Enterococcus faecalis and PBS buffer liquid cotton balls. The two groups were treated with glass ion cements. The healthy left mandibular first molar was the blank control group.A, and the B two groups were randomly selected from 3 rats at 1 weeks, 2 weeks, 3 weeks and 4 Thursday time respectively. The paraformaldehyde was used to remove the soft tissue and the mandible, and the right mandibular first molars and the left mandibular first molar root periapical tissues were scraped with the dug spoon respectively. The tissue RNA was extracted and transcribed into cDNA. The expression level of TNF- alpha mRNA was detected by real-time fluorescence quantitative PCR (Real-timePCR) and analyzed statistically.
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