贝母辛对LPS致黏液高分泌小鼠MUC5AC和EGFR表达的影响

发布时间：2018-05-28 04:05

本文选题：贝母辛 + 黏液高分泌　；参考：《中药药理与临床》2015年03期

【摘要】：目的:探讨脂多糖致黏液高分泌小鼠MUC5AC表达的影响,并初步探讨其机制。方法:用乙醚麻醉小鼠,经鼻腔滴注脂多糖(1 mg/5 ml磷酸缓冲盐溶液,每只50μl),构建小鼠黏液高分泌模型。将小鼠随机分为对照组、脂多糖组和2mg/kg、4mg/kg、6mg/kg剂量贝母辛组。HE染色观察各组小鼠肺组织病理学改变、实时定量PCR法检测MUC5AC mRNA表达、免疫组织化学法检测MUC5AC蛋白表达及表皮生长因子受体蛋白表达。结果:脂多糖组肺组织病理学显示:气道上皮脱落,气道内有粘液分泌,肺泡结构呈实变,有大量炎症细胞渗出,贝母辛治疗组上述变化明显减轻,且具有剂量依赖性。贝母辛(2mg/kg、4mg/kg、6mg/kg)均可降低脂多糖致黏液高分泌小鼠MUC5AC mRNA表达,与脂多糖组相比,均有显著的统计学意义,具有剂量依赖性。免疫组织化学法检测MUC5AC蛋白,正常小鼠气道上皮仅有少量的MUC5AC蛋白表达,模型组明显高于空白组,贝母辛可下调脂多糖诱导的MUC5AC表达,具有剂量依赖性。贝母辛可下调脂多糖诱导的表皮生长因子受体蛋白表达,具有剂量依赖性。结论:贝母辛可降低脂多糖致黏液高分泌小鼠MUC5AC表达,其机制可能与表皮生长因子受体表达下调有关。
[Abstract]:Aim: to investigate the effect of lipopolysaccharide (LPS)-induced mucus hypersecretion on MUC5AC expression in mice and its mechanism. Methods: the mice were anesthetized with ether and injected with 1 mg/5 / ml phosphate buffer solution through nasal cavity to establish a mouse model of hypersecretion of mucus. The mice were randomly divided into control group, lipopolysaccharide group and 2 mg / kg / kg Beimuxin group. The pathological changes of lung tissue were observed by HE staining, and the expression of MUC5AC mRNA was detected by real-time quantitative PCR. The expression of MUC5AC protein and epidermal growth factor receptor protein were detected by immunohistochemistry. Results: in the lipopolysaccharide group, the lung histopathology showed that the airway epithelium was shedding, mucus secreted in the airway, the alveolar structure was solid, and a large number of inflammatory cells exudated. The above changes in the treatment group were obviously alleviated in a dose-dependent manner. The MUC5AC mRNA expression in mice induced by lipopolysaccharide was significantly lower than that in lipopolysaccharide group (P < 0.05). The expression of MUC5AC protein was detected by immunohistochemistry in normal mouse airway epithelium. The expression of MUC5AC protein in model group was significantly higher than that in blank group. Beimuxin could down-regulate the expression of MUC5AC induced by lipopolysaccharide in a dose-dependent manner. Beimuxin can down-regulate the expression of epidermal growth factor receptor protein induced by lipopolysaccharide in a dose-dependent manner. Conclusion: Beimuxin can reduce the expression of MUC5AC in mice induced by lipopolysaccharide and its mechanism may be related to the down-regulation of epidermal growth factor receptor expression.
【作者单位】：江西中医学院基础医学院生理学教研室;江西中医学院药学院药理学教研室;
【基金】：江西省卫生厅中医药科研基金课题,2012A151 江西中医药大学重点学科基金和江西中医药大学道地药材专项基金
【分类号】：R285.5

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