番茄红素对梗阻性黄疸大鼠肝脏保护作用的实验研究

发布时间：2018-06-01 12:38

  本文选题：番茄红素 + 梗阻性黄疸　； 参考：《南华大学》2014年硕士论文

【摘要】：目的： 探讨番茄红素对梗阻性黄疸大鼠肝脏的保护作用，，为番茄红素在临床应用提供理论依据。 材料与方法： 1.动物分组：健康雄性成年SD大鼠30只（由湖南省人民医院临床研究所提供），体重250±20g。饲养环境：温度保持在22℃，湿度保持在50%-60%，干净卫生。操作前后均自由进食、自由饮水，12小时昼夜规律生活。大鼠随机分为3组：SO-MO组：假手术玉米油口服组；BDL-MO组：梗阻性黄疸玉米油口服组；BDL-LP组：梗阻性黄疸番茄红素口服组（番茄红素溶于0.5ml玉米油中）。 2.动物模型制备：各组大鼠：10%水合氯醛按0.3ml/kg腹腔注射麻醉，取仰卧位，四肢固定，络合碘消毒，铺孔单，腹正中白线进腹，找到胆总管。（1）SO-MO:予以翻动第一肝门后关腹。术前3天开始至术后14天连续予口服玉米油0.5ml。（2）BDL-MO组：予以0#丝线结扎胆总管后关腹，术前3天开始至术后14天连续予口服玉米油0.5ml。（3）BDL-LP组：予以0#丝线结扎胆总管后关腹，术前3天开始至术后14天连续予口服番茄红素100mg/Kg/d（溶于0.5ml玉米油中）。所有口服液体均经灌胃器给药。 3.指标检测：（1）术后14天心脏穿刺取血标本监测各组大鼠生化指标（AST、ALT、D-BIL、GGT）。（2）术后14天取各组大鼠肝脏标本监测（MDA、SOD）。（3）术后14天取各组大鼠肝组织标本监测TNF-α。（4）术后14天取各组大鼠肝组织标本监测DNA损伤指标。（5）术后14天取各组大鼠肝脏标本进行组织学监测（汇管区炎症、局灶性坏死）。 结果： 1.各组大鼠血生化指标 （1）与SO-MO比较，BDL-MO组及BDL-LP组大鼠的AST、ALT、GGT、D-BIL明显增高，(P＜0.05)，差异有统计学意义；而BDL-LP组大鼠的ALT、AST、GGT较BDL-MO组明显降低（P＜0.05）；BDL-MO组与BDL-LP组大鼠的D-BIL无明显差异（P＜0.05）。 2.肝组织的（MDA、SOD） (1)肝脏MDA:BDL-MO组MDA明显高于SO-MO组，P0.05，差异有统计学意义；BDL-LP组MDA明显低于BDL-MO组，P＜0.05，差异有统计学意义；SO-MO组与BDL-LP组未见明显差异（P＞0.05）。 (2)肝脏SOD：BDL-MO组明显低于SO-MO组，P＜0.05，差异有统计学意义。BDL-LP组SOD明显低于SO-MO组，P＜0.05，差异有统计学意义；而BDL-LP组的SOD较BDL-MO组均高，P＜0.05，差异有统计学意义。 3.肝组织TNF-α： BDL-MO组TNF-α明显高于SO-MO组（P0.05）；BDL-LP组TNF-α明显低于BDL-MO组（P＜0.05）；SO-MO组与BDL-LP组未见明显差异，P＞0.05,无统计学意义。 4.肝细胞DNA损伤： BDL-MO组的DNA损伤指标（DNA尾长，尾矩，尾强度）均大于SO-MO组，P＜0.05，有统计学意义；BDL-LP组的DNA损伤指标均小于BDL-MO组（P＜0.05），差异有统计学意义；BDL-LP组与SO-MO组DNA指标未见明显差异（P＞0.05）。 5.肝组织的组织学： SO-MO组的肝组织可见大部分汇管区未见炎症细胞浸润，小部分可见少量汇管区炎症细胞浸润。未见局灶性坏死。BDL-MO组的肝组织可见大部分汇管区大量炎性细胞浸润，小部分汇管区少量炎性细胞浸润。可见大量局灶性坏死甚至广泛坏死。BDL-LP组的肝组织绝大多数可见轻微炎症表现，小部分未见局灶性坏死，大部分局灶性坏死，较BDL-MO组明显减轻，P＜0.05，有统计学意义。 结论： 1.使用番茄红素可能能够减轻梗阻性黄疸造成的肝细胞损伤；
[Abstract]:Objective:
Objective to investigate the protective effect of lycopene on the liver of rats with obstructive jaundice, so as to provide a theoretical basis for clinical application of lycopene.
Materials and methods:
1. group of animals: 30 healthy male adult SD rats (provided by Hunan People's Hospital Clinical Research Institute), weight 250 + 20g. feeding environment: temperature kept at 22, humidity kept in 50%-60%, clean and sanitary. Free feeding, free drinking water, 12 hours of day and night regulation of life. Rats were randomly divided into 3 groups: group SO-MO: sham operation jade Oral group of mica oil, group BDL-MO: oral jaundice oral group of obstructive jaundice and group BDL-LP: oral obstructive jaundice lycopene oral group (lycopene dissolves in 0.5ml corn oil).
2. animal model preparation: rats in each group: 10% chloral chloral anaesthesia by 0.3ml/kg intraperitoneal injection, take the supine position, limbs fixed, complex iodine disinfection, paving single, abdominal line into the abdomen, to find the common bile duct. (1) SO-MO: to turn the first hepatic portal after the closure of the abdomen. 3 days before the operation to 14 days after the operation, 0.5ml. (2) BDL-MO group: 0# The silk thread ligated the common bile duct and closed the abdomen after the operation, 3 days before the operation and 14 days after the operation, the oral corn oil 0.5ml. (3) BDL-LP group was given after the operation, and the 0# thread was ligated to the common bile duct and closed the abdomen. The oral lycopene 100mg/Kg/d (dissolved in the 0.5ml corn oil) was given 3 days before the operation, and the oral liquid was given by the gavage apparatus for 14 days after the operation.
3. index test: (1) 14 days after the operation, the blood samples were monitored by cardiac puncture (AST, ALT, D-BIL, GGT). (2) the liver specimens from each group were monitored at 14 days after operation (MDA, SOD). (3) the liver tissue specimens from each group were monitored for TNF- a (4) on the 14 day after the operation (4) on the 14 day after operation to monitor the index of DNA damage in each group of rats. (5) 14 days after operation. The liver specimens of each group were taken for histological monitoring (focal area inflammation and focal necrosis).
Result锛

本文编号：1964210