海藻糖对异氟烷诱导转APP基因小鼠学习记忆功能的影响

发布时间：2018-06-03 07:37

本文选题：海藻糖 + 异氟烷　；参考：《中国老年学杂志》2015年01期

【摘要】：目的探讨吸入麻醉药异氟烷对转APP基因小鼠学习记忆功能的影响及海藻糖的干预保护作用。方法 60只12月龄转APP小鼠随机分为空白对照组(Control组)、异氟烷组(Iso组)、海藻糖组(Iso+Tre组),每组20只。Iso+Tre组和Iso组分别于麻醉前30 min腹腔注射海藻糖400μg/kg或等量生理盐水,然后给予1.4%异氟烷吸入麻醉2 h,麻醉后24 h应用Morris水迷宫测试小鼠学习记忆能力,麻醉后6、24 h杀鼠取脑,采用TUNEL染色观察各组小鼠海马区神经元凋亡,应用ELISA法检测Aβ含量,Western印迹法检测Bcl-2、Bax、Caspase-3的表达变化。结果与Control组相比,Iso组小鼠总体的平均逃避潜伏期明显延长,空间探索时间明显缩短(P0.01),海马神经元凋亡指数、Aβ含量、Bax/Bcl-2比值及Caspase-3表达均明显增加(P0.01);Iso+Tre组与Iso组相比,逃避潜伏期缩短,空间探索时间延长(P0.05),Aβ含量、Bax/Bcl-2比值及Caspase-3表达均明显减少(P0.01)。结论海藻糖能够改善异氟烷诱导转APP基因小鼠学习记忆能力的损害,减少海马神经元的凋亡及Aβ的生成。
[Abstract]:Objective to investigate the effects of inhaled anesthetic isoflurane on learning and memory function of APP transgenic mice and the protective effect of trehalose. Methods Sixty 12-month-old APP mice were randomly divided into control group (n = 20), isoflurane group (n = 20) and Iso group (n = 20). 20 rats in each group were intraperitoneally injected with trehalose 400 渭 g/kg or the same amount of normal saline 30 min before anesthesia. Then the mice were given 1.4% isoflurane inhalation anesthesia for 2 hours, the learning and memory ability of mice was tested by Morris water maze 24 hours after anesthesia, and the rat brain was killed 6 hours after anesthesia. The apoptosis of hippocampal neurons in each group was observed by TUNEL staining. ELISA assay was used to detect the content of A 尾. Western blot was used to detect the expression of Bcl-2 and Baxon Caspase-3. Results compared with the Control group, the average escape latency was significantly prolonged, the spatial exploration time was significantly shortened, and the apoptotic index of hippocampal neurons, the ratio of Bax / Bcl-2 and the expression of Caspase-3 in the hippocampal neurons were significantly increased in the Iso Tre group compared with the Iso group. The escape latency was shortened and the spatial exploration time was prolonged. The ratio of Bax / Bcl-2 and the expression of Caspase-3 decreased significantly. Conclusion trehalose can improve the learning and memory ability of APP transgenic mice induced by isoflurane and decrease the apoptosis of hippocampal neurons and the production of A 尾.
【作者单位】：吉林大学第一医院麻醉科;
【基金】：国家自然科学基金资助项目(No.81271215;No.81141065)
【分类号】：R614

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