恒河猴运动皮层手区切除后运动皮层手面区可塑性变化及针刺的作用观察

发布时间：2018-06-04 17:26

本文选题：恒河猴 + 合谷穴区　；参考：《湖南中医药大学》2014年硕士论文

【摘要】：目的：探讨恒河猴运动皮层手区(支配合谷穴区)切除后运动皮层手面区是否发生可塑性变化,以及针刺对运动皮层手面区可塑性的作用。方法：恒河猴2只,随机分为自然恢复和针刺治疗两组,动物麻醉后呈俯卧位置于大动物脑立体定位仪上,无菌条件下,行开颅术,于前囟前1cm,向左/右旁开3cm处为中心钻一1.5×1.5cm2正方形骨窗,暴露脑组织见中央沟和中央前回。采用自制银球电极刺激运动皮层并同时采用16导电生理记录仪记录肌电以确定运动皮层手区范围,切除支配手部(支配合谷穴区)肌肉的运动皮层后,待其伤口自然愈合/针刺治疗3个月,针刺治疗选用华佗牌一次性无菌针灸针(规格：Φ0.30×25mm,苏州医疗器械厂生产)刺入恒河猴运动皮层手区切除对侧的合谷穴和鱼际穴,深度15mm,针灸针与穴位皮肤呈90°,采用韩式电针仪(HANS-200A型,南京济生医疗科技有限公司)进行电针治疗,选择疏密等幅刺激波形。隔日1次,每次留针20分钟。观察运动皮层手/面区的功能代偿情况及针刺的作用。结果： 1.自然愈合组和针刺治疗组恒河猴在切除运动皮层手区(合谷穴区)三个月后,刺激与手区相交界的面区均可诱导出轻微的对侧拇食指运动,其主要表现为拇指外展或拇食指的颤动,其刺激强度明显大于切除前手区诱发拇食指运动的刺激强度,且伴随有明显的面部运动。 2.针刺治疗组的恒河猴运动皮层手面区可塑性变化范围略大于自然恢复的恒河猴。结论： 1.支配手区(合谷穴区)运动皮层的损伤可导致运动皮层手区的功能被面区所替代,其引发的可塑性变化是渐进性的,且有较明显的趋向性,即产生手区和面区的可塑性变化,而非其他部位。 2.针刺对恒河猴运动皮层手区(合谷穴区)切除后运动皮层手面区可塑性变化可能有一定的作用。
[Abstract]:Objective: to investigate the plasticity of hand area of motor cortex of rhesus monkey after excision and the effect of acupuncture on the plasticity of hand area of motor cortex. Methods: two rhesus monkeys were randomly divided into two groups: natural recovery group and acupuncture group. At 1 cm in front of anterior fontanelle, a 1.5 脳 1.5cm2 square bone window was drilled to the left / right side of 3cm. The central sulcus and precentral gyrus were exposed to the brain tissue. The motor cortex was stimulated by a self-made silver ball electrode and the electromyography was recorded with 16 conductive physiological recorder to determine the range of the hand area of the motor cortex and to remove the motor cortex that innervated the muscles of the Hegu acupoint area of the hand. After 3 months of natural wound healing / acupuncture treatment, Huatuo brand sterile acupuncture needle (桅 0.30 脳 25 mm, produced by Suzhou Medical equipment Factory) was used for acupuncture treatment to cut the contralateral Hegu point and thenar point in the hand area of the motor cortex of rhesus monkey. The depth of 15 mm, acupuncture needle and acupoint skin 90 掳, using the Korean electroacupuncture instrument HANS-200A, Nanjing Jisheng Medical Science and Technology Co., Ltd.) electroacupuncture treatment, select density equal amplitude stimulus waveform. Keep the needle for 20 minutes once every other day. To observe the functional compensation of hand / facial area of motor cortex and the effect of acupuncture. Results: 1. In the natural healing group and acupuncture treatment group, three months after the removal of the hand area of motor cortex (Hegu area), the stimulation of the facial area at the junction of the hand area and the hand region could induce slight contralateral thumb forefinger movement. The stimulation intensity of thumb abduction or thumb index finger was significantly higher than that of forefinger movement induced by excision, and accompanied by obvious facial movement. 2. The range of plasticity in the hand area of the motor cortex in the acupuncture treatment group was slightly larger than that in the naturally recovered rhesus monkey. Conclusion: 1. The damage of the motor cortex in the dominant hand area (Hegu acupoint area) can result in the function of the hand region of motor cortex being replaced by the facial area, and the plasticity change caused by the damage is gradual and has obvious tendency, that is to say, the plasticity of the hand area and the plane area is changed. Not other parts. 2. Acupuncture may play a certain role in the plasticity of hand area of motor cortex of rhesus monkey after excision of Hegu area.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R245

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