大鼠体外循环前后血浆蛋白质差异性表达

发布时间：2018-06-07 13:35

本文选题：血浆 + 蛋白质组学　；参考：《重庆医学》2015年10期

【摘要】：目的实施大鼠体外循环(CPB)前后血浆比较蛋白质组学研究,了解CPB前、后血浆蛋白质的差异性表达,以期发现对早期诊断CPB后并发症有参考价值的血浆标志物。方法将成年雄性SD大鼠10只分为实验组和对照组,每组5只,术前自由进食进水。实验组大鼠建立CPB模型,对照组只进行麻醉和动、静脉穿刺操作。两组动物分别在CPB前和CPB结束时采取血液标本1mL,肝素抗凝,分离血浆。血浆蛋白提纯后进行二维凝胶电泳、用ImageScanner扫描仪扫描成像。将被认定为差异表达的蛋白质斑点进行切胶、酶解和肽段提取后,用质谱仪分析鉴定。结果 CPB后,大鼠血浆蛋白质表达斑点数目明显增加,共有17个表达上调的蛋白质斑点被认定为CPB所致。经质谱分析,共鉴定出5种蛋白质,分别为:凝溶胶蛋白、结合珠蛋白、载脂蛋白A1(ApoA1)、免疫球蛋白和Ba1-647。结论 CPB可引起大鼠血浆蛋白质出现差异性表达,从功能上分析,凝溶胶蛋白、结合珠蛋白、ApoA1有可能作为研究CPB并发症的血浆标志物。
[Abstract]:Objective to investigate the differential expression of plasma protein before and after CPB in rats before and after cardiopulmonary bypass (CPB). Methods Ten adult male Sprague-Dawley rats were divided into experimental group and control group with 5 rats in each group. The CPB model was established in the experimental group and the control group only performed anesthesia and venipuncture. Before CPB and at the end of CPB, the two groups were treated with 1 mL of blood sample, heparin anticoagulant and plasma separation. The plasma protein was purified by two-dimensional gel electrophoresis and scanned by ImageScanner scanner. Protein spots identified as differentially expressed proteins were identified by mass spectrometer after gelatinization, enzymolysis and peptide extraction. Results after CPB, the number of protein expression spots in plasma of rats was significantly increased, and a total of 17 up-regulated protein spots were thought to be caused by CPB. Five kinds of proteins were identified by mass spectrometry, they were agglutinin, binding globin, apolipoprotein A1-ApoA1, immunoglobulin and Ba1-647. Conclusion CPB can induce differential expression of plasma protein in rats. In functional analysis, coagulation sol protein and bound globin ApoA1 may be used as plasma markers to study the complications of CPB.
【作者单位】：重庆医科大学附属儿童医院麻醉科;重庆医科大学附属儿童医院血液肿瘤科;
【基金】：国家自然科学基金资助项目(30700785) 中国博士后科学基金资助项目(200704100779)
【分类号】：R726.1

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