泛素连接酶和小清蛋白在脑性瘫痪小鼠模型海马组织中的表达及其意义

发布时间：2018-06-10 05:54

本文选题：脑性瘫痪 + 泛素连接酶　；参考：《神经解剖学杂志》2015年01期

【摘要】：目的:探讨泛素连接酶(Huwe1)和小清蛋白(Parvalbumin,PV)在脑性瘫痪小鼠海马组织中的表达及意义。方法:新生7 d昆明小鼠,随机分为模型组和对照组,模型组按Rice的方法手术通过结扎小鼠单侧颈总动脉并缺氧来制作脑性瘫痪模型。对照组麻醉后仅切开颈部皮肤,分离单侧颈总动脉,不结扎不剪断,然后缝合伤口,未置于缺氧环境。各组小鼠分别在术后7 d、14 d、21 d、28 d用4%多聚甲醛灌注固定、取脑、作冰冻切片,片厚30μm,应用免疫组化法检测小鼠海马组织中Huwe1和PV的表达。结果:(1)Huwe1和PV在对照组成年小鼠脑中广泛表达,在小鼠发育过程中海马各区Huwe1和PV的表达随着年龄的增加呈上升的趋势,至成年时趋于稳定;(2)模型组小鼠自术后7 d起,模型组损伤侧海马CA1、CA3、DG区Huwe1的表达均高于其对侧和对照组(P0.05);PV的表达虽然高于其对侧,却又低于其对照组(P0.05)。结论:Huwe1在脑性瘫痪小鼠模型损伤侧海马中表达增高可能与脑损伤后其清除异常蛋白有关。PV的低表达可能与脑缺血有关,其损伤侧一过性的高表达提示可能与脑损伤后PV缓冲胞内钙超载的能力增强有关。
[Abstract]:Aim: to investigate the expression and significance of ubiquitin ligase Huwe1) and white protein Parvalbumin (PVV) in hippocampus of cerebral palsy mice. Methods: Kunming mice were randomly divided into model group and control group. The model group was operated on by ligating unilateral common carotid artery and hypoxia to make cerebral palsy model according to Rice's method. In the control group, the neck skin was cut open, the common carotid artery was separated, the common carotid artery was not ligated and cut, then the wound was sutured and not placed in anoxic environment. The mice in each group were fixed with 4% paraformaldehyde on the 7th day, 14th day, 21 day and 28 day after operation. The brain was taken as frozen section and the thickness of the slice was 30 渭 m. The expression of Huwe1 and PV in the hippocampus of the mice was detected by immunohistochemical method. Results the expression of Huwe1 and PV were widely expressed in the brain of adult mice in control group. The expression of Huwe1 and PV in hippocampal regions of the control group increased with the increase of age, and tended to stabilize at adulthood. In model group, the expression of Huwe1 in hippocampal CA1, CA3 and DG region was higher than that in contralateral side and control group, although it was higher than that in contralateral side, it was also lower than that in control group. Conclusion the increased expression of WHuwe1 in the injured hippocampus of cerebral palsy mice may be related to the low expression of abnormal clearance protein after brain injury, which may be related to cerebral ischemia. Transient overexpression in the injured side suggests that the increased ability of PV to buffer intracellular calcium overload may be associated with brain injury.
【作者单位】：徐州医学院人体解剖学教研室;灵璧县人民医院;徐州医药高等职业学校;
【基金】：江苏省大学生科技创新计划(CXLX12-0992)
【分类号】：R742.3

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