静脉注射大肠杆菌制备高动力型感染性休克犬模型

发布时间：2018-06-11 23:20

本文选题：感染性休克 + 动物实验　；参考：《中国比较医学杂志》2017年09期

【摘要】：目的通过静脉注射大肠杆菌方法制备高动力型感染性休克犬模型。方法杂种犬22只,随机分为对照组(n=11)和实验组(n=11)。麻醉犬后,经右股静脉置入双腔中心静脉导管,对照组使用微量泵向犬股静脉内泵入生理盐水,实验组以同样方法泵入大肠杆菌,经右股动脉置入PICCO导管,在0~48 h各时点应用PICCO监测全身血流动力学情况。结果实验组的心率(HR)、心排量(CO)、每搏量(SV)在12 h均显著升高(P0.05),收缩压(SBP)、外周血管阻力(SVR)在12 h显著降低(P0.05),而其他血流动力学指标如中心静脉压(CVP)、肺动脉楔压(PAWP)、氧输送(DO2)、氧消耗(VO2)、氧摄取率(O2ER)差异均无显著性(P0.05)。实验组从12 h起即可观察到明显的尿量减少;动脉收缩期峰值血流速度(PSV)和阻力指数(RI)与对照组相比差异均有显著性(P0.05)。结论运用微量泵向犬股静脉泵入活大肠杆菌可以成功制备高动力型感染性休克犬模型,可为研究感染性休克提供实验基础。
[Abstract]:Objective to prepare a high dynamic septic shock dog model by intravenous injection of Escherichia coli. Methods 22 hybrid dogs were randomly divided into control group (n=11) and experimental group (n=11). After anesthetized dogs, the double lumen central venous catheter was inserted through the right femoral vein, and the control group was pumped into the canine femoral vein by a micropump. The experimental group was given the same method of pump. The PICCO catheter was inserted into the right femoral artery by the right femoral artery, and the whole body hemodynamics was monitored by PICCO at the time point of 0~48 H. The heart rate (HR) and cardiac output (CO) in the experimental group increased significantly (SV) at 12 h (P0.05), the systolic pressure (SBP), and the peripheral blood tube resistance (SVR) decreased significantly in the 12 h. There was no significant difference in cardiac venous pressure (CVP), pulmonary artery wedge pressure (PAWP), oxygen delivery (DO2), oxygen consumption (VO2) and oxygen uptake (O2ER). The experimental group observed significant decrease in urine volume from 12 h; the peak systolic blood velocity (PSV) and resistance index (RI) of the arterial systole were significantly different from the control group (P0.05). Conclusion the use of Micropump A high dynamic septic shock dog model can be successfully prepared by infusion of live Escherichia coli into canine femoral vein. It can provide experimental basis for the study of septic shock.
【作者单位】：大连医科大学附属大连市中心医院重症医学科;
【基金】：大连市卫计委2013年课题项目(课题名称:《亚低温治疗感染性休克所致ARDS的实验研究》)
【分类号】：R-332;R459.7

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