高脂饮食诱导的肥胖易感及肥胖抵抗大鼠血清网膜素-1水平比较及意义

发布时间：2018-06-13 00:30

本文选题：肥胖易感 + 肥胖抵抗　；参考：《山东医药》2015年40期

【摘要】：目的比较高脂饮食诱导的肥胖易感及肥胖抵抗大鼠血清网膜素-1(omentin-1)水平,并探讨其意义。方法选择雄性Wistar大鼠40只,随机抽取10只作为对照组,饲以标准颗粒饲料;其余30只饲以高脂饲料。饲养12周后,从以高脂饲料饲养的大鼠中选取体质量增加最多的10只作为肥胖易感组、体质量增加最少的10只作为肥胖抵抗组,其余剔除。各组称取体质量,测量鼻尖至肛门的距离,计算Lee's指数。麻醉后,心脏取血,检测各组omentin-1水平及血糖、血脂(TG、TC、LDL-C、HDL-C)、胰岛素,计算胰岛素抵抗指数(HOMA-IR)。处死大鼠,称尸重,并迅速摘取附睾周围及肾周脂肪,称湿重,计算体脂比。结果饲养12周后,肥胖易感组血清omentin-1水平为(53.38±7.51)ng/L,明显低于肥胖抵抗组、对照组[(70.33±7.00)、(68.23±6.85)ng/L,P均0.05],而肥胖抵抗组、对照组比较差异无统计学意义(P0.05)。与对照组、肥胖抵抗组比较,肥胖易感组体质量、体脂比、Lee's指数、HOMA-IR及血清TG水平均明显升高(P均0.05),而肥胖抵抗组与对照组各指标比较差异均无统计学意义(P均0.05)。相关分析发现,血清omentin-1水平与Lee's指数、HOMA-IR均呈负相关(r分别为-0.457、-0.569,P均0.05)。结论高脂饮食诱导的肥胖易感大鼠血清omentin-1水平明显升高;omentin-1在抵制肥胖的发生中具有重要作用。
[Abstract]:Objective to compare the serum levels of omentin-1 (omentin-1) in obesity prone and obese resistant rats induced by high fat diet and to explore its significance. Methods Forty male Wistar rats were randomly selected as control group and 30 rats were fed with high fat diet. After 12 weeks of feeding, 10 rats with the most increased body mass were selected as obesity susceptible group, 10 rats with the least increase in body mass were selected as obesity resistance group, and the rest were excluded from the rats fed with high fat diet. The distance between nasal tip and anus was measured and the Leehs index was calculated. After anesthesia, the blood was taken from the heart, the levels of omentin-1 and blood glucose, the serum lipids of TGG, LDL-C, HDL-C, insulin, and the insulin resistance index (HOMA-IRI) were measured. The rats were killed, weighed, and quickly removed the peri-epididymal and perirenal fat, weighed wet, and calculated the ratio of body fat to fat. Results after 12 weeks of feeding, the serum omentin-1 level in the obesity susceptible group was 53.38 卤7.51 ng / L, which was significantly lower than that in the obese resistance group, and in the control group (70.33 卤7.00 卤68.23 卤6.85 ng / L P 0.05), but there was no significant difference between the two groups in the obesity resistance group (P 0.05). Compared with the control group and the obesity resistance group, the body weight, body fat ratio and the level of serum TG and HOMA-IR increased significantly in the obesity susceptible group, but there was no significant difference between the obesity resistance group and the control group (P 0.05). Correlation analysis showed that there was a negative correlation between serum omentin-1 level and HOMA-IR of Leehs index (r = -0.457- 0.569P, P = 0.05). Conclusion the level of serum omentin-1 in obesity prone rats induced by high fat diet is significantly increased and the level of omentin-1 plays an important role in resisting the occurrence of obesity.
【作者单位】：中国医科大学附属第四医院;
【分类号】：R589.2

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