七氟醚预处理对大鼠急性肾缺血—再灌注损伤保护作用的实验研究

发布时间：2018-06-14 15:59

本文选题：七氟醚 + 缺血-再灌注损伤　；参考：《吉林大学》2014年硕士论文

【摘要】：目的探讨七氟醚对急性肾缺血-再灌注损伤肾功能的保护作用。方法选择健康SD大鼠90只，，随机分为三组：伪手术组（A组），对照组（B组）及七氟醚预处理组（C组），建立肾脏缺血-再灌注模型,对照组和七氟醚预处理组实验大鼠用10％水合氯醛0.3ml/100g腹腔注射，游离左右肾蒂，夹闭肾蒂阻断肾脏血流45min后解除夹闭，使血流再通，缺血-再灌注损伤模型建立成功后，分别测定各组大鼠平均动脉压（MAP）、二氧化碳分压(PCO2)、血清尿素氮（BUN）、肌酐（Cr）、超氧化物歧化酶(SOD)及胱抑素C(CysC)的变化，HE染色观察各组大鼠肾组织病理改变。所得数据采用SPSS13.0统计学软件分析，P＜0.05有统计学意义。结果随着七氟醚预处理浓度逐渐增加，大鼠的平均动脉压呈逐渐下降的趋势，但在七氟醚浓度为2%-3%的临床常用剂量范围内，大鼠的MAP波动于92.1±6.0mmHg。各组缺血前与再灌注后，PCO2浓度没有明显的差异。B组和C组BUN、Cr于再灌注后4h、12h、24h均明显高于A组（P＜0.05），但C组同一时间点的BUN、Cr水平均较B组明显下降（P＜0.05）。C组和B组均较A组血清SOD活力明显减低（P＜0.05），但C组SOD活力较B组高，差异有统计学意义（P＜0.05）。肾脏病理观察发现：A组肾脏组织结构完整，B组肾脏外髓部分组织结构严重破坏，而C组肾脏组织破坏较小，肾小管组织相对较为完整。本次试验中选取实验动物血清胱抑素C(Cystatin C，Cys C)的变化作为反应对肾脏缺血-再灌注保护作用的指征。与伪手术组相比，对照组、七氟醚预处理组的血清胱抑素C均明显升高（P〈0.05）；与对照组相比，七氟醚预处理组的血清胱抑素C水平有所降低（P〈0.05）。结论七氟醚吸入式麻醉能够有效降低BUN、Cr、CysC水平，升高血清SOD活力，从而对急性肾缺血-再灌注损伤肾功能具有一定的保护作用。
[Abstract]:Objective to investigate the protective effect of sevoflurane on renal function after acute renal ischemia-reperfusion injury. Methods 90 healthy SD rats were randomly divided into three groups: sham operation group (group A), control group (group B) and sevoflurane preconditioning group (group C) to establish renal ischemia-reperfusion model. In the control group and sevoflurane pretreatment group, 10% chloral hydrate (0.3ml/100g) was injected intraperitoneally to free the left and right renal pedicles, and the renal pedicle was blocked by blocking the renal blood flow (45min), then the blood flow was re-opened, and the model of ischemia-reperfusion injury was established successfully. The mean arterial pressure (MAPP), carbon dioxide partial pressure (CO 2), bun (bun), creatinine (Cr), superoxide dismutase (SOD) and cystatin CysC (Cy CysC) were measured respectively. The pathological changes of renal tissue in each group were observed by HE staining. The data were analyzed by SPSS 13.0 software (P < 0.05). Results with the increase of preconditioning concentration of sevoflurane, the mean arterial pressure of rats decreased gradually, but the map of rats fluctuated at 92.1 卤6.0 mmHg in the range of 2 to 3% of the concentration of sevoflurane. There was no significant difference in the concentration of PCO _ 2 between groups before ischemia and after reperfusion. The level of bun Cr in group B and group C was significantly higher than that in group A at 4 h after reperfusion (P < 0.05), but the level of bun Cr in group C at the same time point was significantly lower than that in group B (P < 0.05) and group B (P < 0.05). The serum SOD activity of group A was significantly lower than that of group A (P < 0.05), but the activity of SOD in group C was higher than that in group B. The difference was statistically significant (P < 0.05). The pathological observation of kidney showed that the renal tissue structure was intact in group A and the structure of extramedullary tissue in group B was serious, while that in group C was less than that in group C. the renal tubular tissue was relatively intact. The changes of cystatin Cystatin Cys Cys in serum of experimental animals were selected as the indication of the protective effect of cystatin Cystatin Cys on renal ischemia reperfusion. Compared with the sham operation group, the serum cystatin C level in the sevoflurane preconditioning group was significantly higher than that in the control group (P < 0.05), and the serum cystatin C level in the sevoflurane preconditioning group was lower than that in the control group (P < 0.05). Conclusion sevoflurane inhaled anesthesia can effectively reduce the level of CysC and increase the activity of SOD in serum, which can protect renal function from acute renal ischemia-reperfusion injury.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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