术后应用四逆汤和多奈哌齐对老龄大鼠术后认知的影响

发布时间：2018-06-18 10:15

本文选题：认知障碍 + 应激免疫反应　；参考：《山东中医药大学》2014年硕士论文

【摘要】：目的：通过麻醉行脾切除术建立POCD大鼠模型，探讨POCD的发病机制，以及评价术后应用四逆汤与多奈哌齐对POCD的疗效。方法：选取符合实验要求的健康老龄雄性SD大鼠48只，体重550-650g，随机分为3组，为四逆汤组(A组)、多奈哌齐组(B组)和对照组(C组)。术前连续六天进行水迷宫训练，旷场试验。在相同的条件下用异氟醚麻醉行脾切除术。麻醉过程持续4h。术后待大鼠清醒后给予相应的药物灌胃，术后注意保温。术后连续灌胃七天。术后1d行水迷宫及旷场试验，于术前1天、术后即刻、术后24h静脉取血。术后3，7，21d只行水迷宫及旷场试验。最后取脑并切片，用免疫组化法检测β-AP的表达。抽取的血液进行ACTH、ALD、TNF-α水平及NSE、S-100β的含量测定。结果：手术过程中大鼠生命体征平稳，水迷宫实验结果表明，手术结束后第1、3天，A组大鼠逃避潜伏期明显短于B组和C组(P0.01)。旷场实验结果：术后1d，A组的跨格总数、站立次数、排便次数与另两组相比具有明显差异，均具有统计学意义。而对于TNF-α，ACTH，ALD，NSE，S100β指标，三组较术前均有增加，C组较A、B组的增加更明显；β-AP的沉积亦如此。结论：结果术后应用四逆汤可有效抑制术后应激反应的发生，减少大脑中β-淀粉样蛋白的沉积；降低术后认知障碍发生率；术后应用多奈哌齐可增加大鼠脑海马区胆碱乙酰转移酶及烟碱型乙酰胆碱受体的表达，，亦可减少大脑中β一淀粉样蛋白的沉积，对术后认知功能障碍可起到一定的防治作用。
[Abstract]:Objective: to establish a rat model of POCD by splenectomy under anesthesia, to explore the pathogenesis of POCD and to evaluate the effect of Sini decoction and Donepezil on POCD after operation. Methods: 48 male Sprague-Dawley (SD) rats, weighing 550-650 g, were randomly divided into three groups: Sini decoction group (group A), Donepezil group (group B) and control group (group C). Water maze training and open field test were performed for 6 days before operation. Splenectomy was performed with isoflurane anesthesia under the same conditions. The anesthesia lasted for 4 hours. After operation, the rats were given the appropriate medicine after waking up, and the heat preservation was noticed after operation. Postoperative gastric perfusion for 7 days. Water maze test and open field test were performed 1 day after operation, 1 day before operation, immediately after operation and 24 hours after operation. Water labyrinth and open field test were performed only 3 days and 7 days after operation. Finally, the expression of 尾-AP was detected by immunohistochemical method. The level of TNF- 伪 and the content of NSES-100 尾 in ACTHG ALDN were determined. Results: the vital signs of rats were stable during the operation. The results of water maze test showed that the escape latency of rats in group A was significantly shorter than that in group B and group C on the 1st day after operation. The results of open field experiment showed that there were significant differences in the total number of translattice, standing times and defecation times between group A and the other two groups on the 1st day after operation, all of which were statistically significant. But for TNF- 伪 ACTHN, NSES100 尾, the increase of S100 尾 in all three groups was more obvious than that in group A B, and so was the deposition of 尾 -AP. Conclusion: the post-operative application of Sini decoction can effectively inhibit the occurrence of post-operative stress response, reduce the accumulation of 尾 -amyloid protein in the brain, and reduce the incidence of postoperative cognitive impairment. The expression of choline acetyltransferase and nicotinic acetylcholine receptor and 尾 -amyloid protein deposition in the brain of rats were increased after operation with Donepezil, which may play a role in the prevention and treatment of postoperative cognitive dysfunction.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R619

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