芫花根醇提颗粒联合甲基泼尼松龙对脊髓损伤大鼠BDNF、NMDA表达及行为学的影响

发布时间：2018-06-18 18:08

本文选题：芫花根醇提颗粒 + 甲基泼尼松龙　；参考：《中国中西医结合杂志》2015年08期

【摘要】：目的研究芫花根醇提颗粒(金腰带)联合甲基泼尼松龙对脊髓损伤(spinal cordinjury,SCI)大鼠行为学、脑源性神经营养因子(brain derived neurotrophic factor,BDNF)表达及N-甲基-D-门冬氨酸(N-methyl-D-aspartate,NMDA)水平的影响。方法 SD成年雄性大鼠,随机分为假手术组、脊髓挫伤组(模型组)、金腰带组[灌胃金腰带50 mg/(kg·d),共5天]、甲基泼尼松龙组(脊髓钝挫伤术后8 h内肌肉注射50 mg/kg甲基泼尼松龙,此后甲基泼尼松龙剂量每天减少10 mg/kg,共5天)及联合组(金腰带和甲基泼尼松龙联合干预)。各组大鼠均在术后(0、3、7、28天)进行后肢行为学评价[神经行为学(Basso Beattie and Bresnahan,BBB)评分]。术后13天,每组取8只大鼠,麻醉后取损伤脊髓T_(8-10)液氮中保存,采用[~3H]MK-801放射性配基分析法测定NMDA受体亲和力(Kd)和最大结合数量(Bmax);取术后28天大鼠损伤脊髓,采用免疫组织化学法检测BDNF表达。结果与假手术组比较,模型组腹角和背角BDNF阳性神经元数量升高,Bmax(470±34)升高,Kd降低,术后3~28天BBB评分降低,差异有统计学意义(P0.05)。与模型组比较,各给药组BDNF阳性神经元数量及Kd升高,术后3~28天BBB评分升高(P0.05),Bmax[甲基泼尼松龙组:660±15,金腰带组:646±25,联合组:510±21]降低(P0.05)。与甲基泼尼松龙组比较,金腰带组及联合组BDNF阳性神经元数量及Kd升高(P0.05),术后7~28天BBB评分升高(P0.05),Bmax降低(P0.05)。与金腰带组比较,联合组BDNF阳性神经元数量及Kd升高(P0.05),Bmax降低(P0.05)。结论金腰带能有效改善SCI大鼠后肢运动功能,增加脊髓组织中BDNF的表达,通过影响脊髓NMDA受体减轻脊髓继发性损伤,对SCI有一定的治疗与保护作用,效果优于甲基泼尼松龙且与其有协同作用。
[Abstract]:Objective to study the effects of Daphne genkwa root alcohol extract granule (gold belt) combined with methylprednisolone on behavior, brain derived neurotrophic factor (BDNF) expression and N-methyl-Daspartate N-methyl-D-aspartate (NMDAs) level in spinal cord injury (sci) rats. Methods Sprague-Dawley adult male rats were randomly divided into sham operation group, spinal cord contusion group (model group), golden belt group [intragastric infusion of 50 mg/(kg belt for 5 days] and methylprednisolone group (50 mg/kg methylprednisolone intramuscularly injected within 8 hours after spinal cord contusion). After that, the dosage of methylprednisolone was reduced by 10 mg / kg per day for 5 days) and combined group (combined with gold belt and methylprednisolone). All the rats in each group were evaluated for the behavior of the hind limbs on the 28th day after operation. [the neurobehavioral score was Basso Beattie and Bresnahan BBB]. On the 13th day after operation, 8 rats in each group were taken from each group. The injured spinal cord was preserved in liquid nitrogen after anesthesia. The affinity of NMDA receptor (KdD) and the maximum binding amount (Bmax1) were determined by [3H] MK-801 radioligand assay, and the injured spinal cord was harvested 28 days after operation. The expression of BDNF was detected by immunohistochemistry. Results compared with the sham-operated group, the number of BDNF positive neurons in the ventral horn and dorsal horn of the model group was increased and the number of BDNF positive neurons in the ventral horn and dorsal horn of the model group was increased. The number of BDNF positive neurons in the model group was significantly higher than that in the sham operation group (P < 0.05). Compared with the model group, the number of BDNF positive neurons and KD increased in each administration group, and the BBB score increased 328 days after operation [methylprednisolone group: 660 卤15, gold belt group: 646 卤25, combined group: 510 卤21] decreased P0.05. Compared with methylprednisolone group, the number of BDNF positive neurons and KD in gold belt group and combined group were increased (P 0.05). Compared with the gold belt group, the number of BDNF positive neurons and the increase of KD in the combined group decreased P0.05B _ (max). Conclusion Gold belt can effectively improve the motor function of hind limbs and increase the expression of BDNF in spinal cord tissue of sci rats. It can reduce the secondary injury of spinal cord by affecting NMDA receptor of spinal cord and has a certain therapeutic and protective effect on sci. The effect was superior to and synergistic with methylprednisone.
【作者单位】：云南省玉溪市中医医院;昆明医学院神经科学研究所;云南省昆明市第一人民医院神经外科;
【基金】：玉溪市科技合作专项计划项目
【分类号】：R285.5

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