4-硝基苯酚对大鼠肾上腺皮质孕酮分泌的影响

发布时间：2018-06-19 16:43

本文选题：-硝基苯酚 + 雄性大鼠　；参考：《南京农业大学学报》2015年03期

【摘要】：[目的]设计体内体外试验研究环境内分泌干扰物4-硝基苯酚(4-nitrophenol,PNP)对雄性大鼠肾上腺皮质孕酮分泌的影响。[方法]体内试验采用24只SPF级雄性未成年SD大鼠,随机分为4组,连续2周皮下注射(剂量为0、0.1、1.0和10.0 mg·kg-1的PNP)。最后一次注射24 h后称体质量,然后麻醉处死,收集血液及各脏器,以备测定;为进一步确定PNP对肾上腺皮质孕酮分泌的影响,体外试验利用肾上腺皮质细胞原代培养,细胞贴壁后用不同浓度的PNP(0、10-6、10-5和10-4mol·L-1)培养24 h。[结果]体内试验结果显示:0.1和1.0 mg·kg-1PNP处理组大鼠脾脏质量显著低于对照组(P0.05),但是体质量及其他脏器质量和脏器指数均无显著性差异(P0.05);10.0 mg·kg-1组大鼠血清睾酮水平显著升高(P0.05),而孕酮水平显著降低(P0.05),雌二醇水平差异不显著(P0.05);光学显微镜组织学检查未发现注射PNP组肾上腺皮质形态异常,但RT-PCR结果显示10.0 mg·kg-1PNP处理大鼠肾上腺中St AR、P450scc和P450c17 mRNA表达显著增加(P0.05),而3β-HSD mRNA表达显著降低(P0.05)。体外试验结果显示:与对照组相比,注射PNP组培养液中孕酮水平显著降低(P0.05)。[结论]4-硝基苯酚可能主要通过影响肾上腺类固醇合成酶的表达来影响肾上腺皮质孕酮的分泌功能。
[Abstract]:[objective] to study the effect of environmental endocrine disruptor 4-nitrophenolol (PNPP) on progesterone secretion in adrenal cortex of male rats in vitro and in vivo. [methods] Twenty-four SPF male adult SD rats were randomly divided into 4 groups for 2 weeks by subcutaneous injection (dose of 0.1mg kg-1 and 10.0 mg kg-1). In order to determine the effect of PNP on the secretion of progesterone, the primary culture of adrenocortical cells was used in vitro to determine the effect of PNP on the secretion of progesterone. The cells were cultured for 24 h with different concentrations of 10 ~ (-6) C 10 ~ (-5) and 10-4mol L ~ (-1). [results] the results of in vivo test showed that the spleen weight of rats treated with 0.1 and 1.0 mg 路kg -1 PNP was significantly lower than that of control group (P 0.05), but there was no significant difference in body weight, other organ mass and organ index. The level of progesterone decreased significantly (P 0.05), while estradiol level was not significantly different from that of P0.05.The histological examination of optical microscope did not show that the adrenal cortex was abnormal in PNP injection group, but the level of progesterone was significantly lower than that of P0. 05% (P < 0. 05). However, the results of RT-PCR showed that the expression of P450 scc and P450 C17 mRNA in the adrenal gland of rats treated with 10.0 mg kg-1 PNP increased significantly, while the expression of 3 尾 -HSD mRNA decreased significantly. The results of in vitro test showed that the level of progesterone in PNP group was significantly lower than that in control group. [conclusion] 4-nitrophenol may affect the secretion of progesterone by affecting the expression of adrenal steroid synthetase.
【作者单位】：南京农业大学动物科技学院;
【基金】：国家“十二五”科技支撑计划项目(2012BAD39B02)
【分类号】：S859.81

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