小鼠膀胱内压力与尿道外括约肌肌电图同步测定方法的建立与验证

发布时间：2018-06-19 18:10

本文选题：下尿路症状 + 阴部神经　；参考：《第三军医大学学报》2017年02期

【摘要】：目的建立小鼠膀胱内压力(cystometrography,CMG)与尿道外括约肌肌电图(external urethral sphincter electromyography,EUS EMG)同步测定的方法,并验证其可靠性。方法将12只年龄、体质量相仿的雌性小鼠麻醉后予以耻骨上膀胱造漏并植入导管,然后进行CMG和漏尿点压(leak point pressure,LPP)测定与同步EUS EMG记录。完毕后离断双侧阴部神经,再次重复CMG、LPP及EUS EMG测定,以检测EUS EMG信号的准确性。神经损伤前后计量资料之间的比较采用配对t检验。结果在未损伤阴部神经时,EUS EMG在LPP处的振幅和频率[(111.7±19.4)μV和(142.6±15.0)Hz]较基础膀胱压时的振幅和频率[(38.9±8.4)μV和(37.6±9.5)Hz]有明显的增加(P0.001)。定量分析排尿期所出现的"EUS EMG间断性扑动"信号发现:振幅与频率分别为(70.4±15.2)μV和(393.8±118.9)Hz,单次扑动的持续时间:(85.60±6.21)ms,两个扑动信号之间的间隔时间:(222.0±44.2)ms。而双侧阴部神经离断后,EUS EMG信号几乎完全不能被检测到,说明肌电图信号的真实性。另外,LPP、顶点膀胱压与排尿时的最大膀胱压在离断双侧阴部神经后出现了显著的降低,分别为[(16.4±1.2)cm H2O vs(7.4±0.7)cm H2O,P0.001;(26.7±1.7)cm H2O vs(15.9±1.1)cm H2O,P0.001;(35.5±2.0)cm H2O vs(22.8±1.1)cm H2O,P0.001]。结论成功建立并验证了CMG与EUS EMG同步测定在小鼠模型上的可行性。
[Abstract]:Objective to establish a method for the simultaneous determination of cystometrography (CMG) and external urethral sphincter electromyography (EUS EMG) in mice, and to verify its reliability. Methods 12 female mice with similar body mass were used to make the suprapubic bladder leak and implant the catheter, and then C. MG and leak urine point pressure (leak point pressure, LPP) and synchronous EUS EMG record. After completion, bilateral pudendal nerves were disconnected, CMG, LPP and EUS EMG were repeated to detect the accuracy of EUS signals. Amplitude and frequency of amplitude and frequency [(111.7 + 19.4) mu V and (142.6 + 15) Hz] (38.9 + 8.4) mu V and (37.6 + 9.5) Hz] were significantly increased (P0.001). Quantitative analysis of the "EUS EMG intermittent flutter" signals during the urination period found that amplitude and frequency were (70.4 + 15.2) mu V and (393.8 + 118.9) Hz respectively, and the duration of single flutter was sustained. Room: (85.60 + 6.21) ms, interval time between two flapping signals: (222 + 44.2) Ms. and bilateral pudendal nerve after severed, EUS EMG signal can hardly be detected, indicating the authenticity of electromyography signal. In addition, LPP, the maximum bladder pressure at the vertex and urination at the disconnection of the bilateral pudendal nerve. Not for [(16.4 + 1.2) cm H2O vs (7.4 + 0.7) cm H2O, P0.001; (26.7 + 1.7) cm H2O vs (15.9 + 1.1) cm H2O, P0.001; (35.5 + 2) (22.8 + 1.1).
【作者单位】：第二军医大学长海医院泌尿外科;
【基金】：国家自然科学基金青年科学基金(81500579)~~
【分类号】：R69

【相似文献】