依托咪酯对大鼠海马CA1区兴奋性突触后电流及离子通道的影响

发布时间：2018-06-21 09:53

本文选题：海马CA1区 + 兴奋性突触后电流　；参考：《新疆医科大学》2014年硕士论文

【摘要】：目的：观察静脉麻醉药物依托咪酯对大鼠海马CA1区兴奋性突触后电流(excitatory postsynaptic current, EPSC)及离子通道的影响,探索全麻原理及中枢作用机制。方法：10只成年雄性Wistar大鼠迅速断头后分离海马组织,运用低温切片机制作400gm厚度的海马脑切片40片,选择完整均匀的脑片20片(n=20),遂随机分为2组置于60μl脂肪乳剂(n=10)或10μmol/L依托咪酯乳剂(n=10)中培养40分钟,后置入膜片钳载物槽中,在Schaeffer侧支／联合纤维处给予单刺激(刺激强度0.1nA),间隔20s后改变膜钳制电位(-100mV～+40mV,变化幅度为20mV),再次给予单刺激,记录翻转电位,EPSC幅值与频率,采用细胞贴附式膜片钳记录技术全程记录单通道氯电流频谱。结果：保持膜钳制电位在-60mV时,脂肪乳剂组与依托咪酯组相比,氯离子翻转电位由-53mV左右降低为-60mV左右；全程中EPSC幅值、发生频率较脂肪乳剂组均降低(P0.05)；依托咪酯组单通道氯电流发生明显增强,通道开放程度增加(P0.05)。结论：依托咪酯主要作用于突触后膜GABA受体,通过抑制EPSC的发生和程度达到抑制性突触传导的作用;降低膜翻转电位,使膜电位超极化；此过程中离子内流的程度增加,阴离子通道开放概率升高,这种膜内外环境的改变可能为氯离子的跨膜转运所造成。
[Abstract]:Aim: to observe the effect of etomidate on excitatory postsynaptic current (EPSCT) and ion channel in hippocampal CA1 region of rats, and to explore the mechanism of general anesthesia. Methods Hippocampal tissue was isolated from 10 adult male Wistar rats after decapitation, and 40 slices of 400gm thick hippocampal brain were made by hypothermia slicing machine. 20 slices of brain slices were randomly divided into two groups, which were cultured in 60 渭 l fat emulsion or 10 渭 mol / L etomidate emulsion for 40 minutes, then placed in the patch clamp slot. The Schaeffer lateral branch / combined fibers were given single stimulation (stimulation intensity 0.1 nAN, interval 20 s). The membrane clamping potential was changed from 100 MV to 40 MV, and the amplitude of change was 20 MV. The amplitude and frequency of EPSC were recorded, and the amplitude and frequency of EPSC were recorded. Single channel chlorine current spectrum was recorded by cell attached patch clamp recording technique. Results: compared with etomidate group, the chloride ion turnover potential of fat emulsion group was decreased from -53 MV to -60 MV, the amplitude of EPSC and the frequency of occurrence were lower than that of fat emulsion group (P 0.05). In etomidate group, the single channel chlorine current was significantly increased, and the opening degree of the channel was increased (P 0.05). Conclusion: etomidate mainly acts on GABA receptor of postsynaptic membrane, which inhibits synaptic conduction by inhibiting the occurrence and degree of EPSC, reduces membrane turnover potential and makes membrane potential hyperpolarization, and increases the degree of ion influx during this process. The opening probability of anion channel is increased, and the change of the environment inside and outside the membrane may be due to the transport of chloride ions across the membrane.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R614

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