腧穴“解郁方”的筛选及其对抑郁模型大鼠HPA轴的影响

发布时间：2018-06-22 21:05

本文选题：抑郁症 + 针刺　；参考：《山西中医学院》2014年硕士论文

【摘要】：目的本研究在大量挖掘、总结文献的基础上，紧密结合临床实际，选定使用频次较高的百会、神门、太冲三穴，分析其对大鼠行为学（体重变化、糖水偏爱度、旷场实验）的影响，筛选出针灸治疗抑郁症的最佳腧穴组方，为临床应用提供科学的实验室依据。同时利用筛选出的最佳腧穴组方研究其对抑郁模型大鼠HPA轴的血清促肾上腺皮质激素(ACTH)、皮质醇(CORT)水平的影响，探讨最佳腧穴组方治疗抑郁症的机制。方法先通过旷场实验将水平运动次数加垂直运动次数不足30次或超过120次的大鼠剔除，实验一：将90只清洁级SD雄性大鼠随机分为空白组A组（n=10)、模型组B组（n=10）、针刺干预组为7个亚组，即C～I组（每组n=10）。7个亚组取穴为：C组（百会组）；D组（神门组）；E组（太冲组）；F组（百会+神门组）；G组（神门+太冲组）；H组（百会+太冲组）；I组（百会+神门+太冲组）。模型组和针刺干预组大鼠采用长期慢性不可预知应激刺激结合孤养的方法造模，大鼠共接受21天不同的刺激，造模的同时针刺干预组即给予应激造模前1h针刺治疗，治疗结束后通过测定大鼠的行为学评分，筛选出针刺治疗抑郁症的最佳腧穴组方，并命名为腧穴解郁方。实验二：在实验一中,我们已经筛选出了最佳腧穴组方，称其为解郁方。在此基础上，选用30只清洁级SD雄性大鼠作为研究对象，随机分为空白组（n=10)、模型组（n=10)、腧穴解郁方组（n=10)。造模同实验一，腧穴解郁方组从造模的同时即给予应激造模前1h针刺治疗，空白组正常喂养，模型组虽经抓取、固定，，但不做针刺，其它处理同解郁方组。治疗结束后规定时间内麻醉取血4ml，采用酶联免疫吸附法（ELISA）检测各组大鼠血清促肾上腺皮质激素(ACTH)、皮质醇(CORT)含量的变化。结果 1.行为学评分结果显示：实验前各组大鼠体重变化，糖水消耗量，旷场实验结果显示均无明显差异（p0.05)。 (1)体重的变化：与空白组比较，实验第7天、第14天、第21天，模型组大鼠的体重均明显下降（p0.05，p0.01）；与模型组比较，实验第7天，百会组、神门组、太冲组、百会+太冲组、三穴组大鼠体重显著增加（p0.05），百会+神门组、神门+太冲组大鼠体重亦高于模型组，但无显著性差异（p0.05)。实验第14天，各针刺组大鼠体重均显著上升（p0.05，p0.01），实验第21天,各针刺组大鼠体重增加较第14天更加显著（p0.01，p0.05），其中三穴组大鼠的体重明显高于其余各针刺组（p0.05）。 (2)实验结束后，糖水消耗量的变化：与空白组相比,模型组的糖水消耗量明显降低(p0.01)；与模型组相比，3穴组大鼠的糖水偏爱度显著升高（p0.05），其余各针刺组大鼠的糖水偏爱度也有所增加但与模型组比较无显著差异（p0.05)。（3）实验结束后，旷场实验评分：与空白组相比，模型组大鼠旷场实验评分均明显下降，有显著差异(p0.01，p0.05)；与模型组比较，各针刺干预组组旷场实验评分均显著增高（p0.01，p0.05)且三穴组明显高于其余各针刺组（p0.05)。 2.电针腧穴解郁方对抑郁模型大鼠HPA轴影响机制的研究结果显示：与空白组比较，模型组ACTH、CORT的含量均明显升高，结果有显著性差异（P0.01）；与模型组比较，腧穴解郁方组ACTH、CORT的含量均明显下降，结果有显著性差异（P0.01）。结论 1.筛选出针刺治疗抑郁症的最佳腧穴组方为百会+神门+太冲。 2.电针腧穴解郁方能够调整大鼠血清中ACTH、CORT的含量，故腧穴解郁方的治疗机制可能与调节HPA轴的功能有关。
[Abstract]:Objective To study the effects of acupuncture and moxibustion on the behavior of rats ( body weight change , sugar water preference , open field experiment ) and to screen out the influence of acupuncture and moxibustion on the behavior of rats ( body weight change , sugar water preference , open field experiment ) . The effect of acupuncture on the behavior of rats ( body weight change , sugar water preference , open field experiment ) was analyzed . The effect of acupuncture on the serum adrenocorticotropic hormone ( ACTH ) and cortisol ( CORT ) level in the HPA axis of the depression model rats was studied .

Methods 90 clean - grade SD male rats were randomly divided into two groups : group A ( n = 10 ) , model group B ( n = 10 ) , acupuncture intervention group 7 subgroups , i.e . group C ~ I ( n = 10 ) .
Group D ( Shenmen Group ) ;
Group E ( Tai Chong Group ) ;
Group F ( Baihui + Shenmen Group ) ;
Group G ( Shenmen + Taichong Group ) ;
H group ( Baihui + Tai Chong group ) ;
The rats were randomly divided into two groups : blank group ( n = 10 ) , model group ( n = 10 ) , acupuncture treatment group ( n = 10 ) .

Results

1 . The results of ethology showed that there was no significant difference in body weight change , sugar water consumption and open field experiment in the rats before the experiment ( p < 0.05 ) .

( 1 ) Body weight : Compared with blank group , the weight of rats in model group decreased significantly ( p < 0.05 , p0.01 ) on day 7 , day 14 and 21 day of experiment ;
Compared with the model group , the weight of the rats in the acupuncture group was significantly higher than that in the model group ( P < 0.05 ) . The weight of the rats in the acupuncture group increased significantly ( P < 0.05 ) . The weight of the rats in the acupuncture group increased significantly ( P < 0.01 , P < 0.01 ) on the 14th day after the experiment .

Nitrates ( p < 0.05 ) .

( 2 ) After the end of experiment , the consumption of sugar water was changed : compared with blank group , the consumption of sugar and water in the model group was obviously lower than that in blank group ( p0.01 ) ;
Compared with the model group , the preference degree of sugar - water in 3 - point group increased significantly ( p < 0.05 ) , and the preference of sugar - water in the remaining acupuncture group was increased but not significantly different from the model group ( p < 0.05 ) .

( 3 ) After the experiment was finished , the open field experiment score : Compared with the blank group , the experimental scores of the open field of the model group were significantly lower than those in the blank group ( p < 0.01 , p < 0.05 ) .
Compared with the model group , the experimental scores of the open field of each acupuncture intervention group were significantly higher ( p0.01 , p0.05 ) and the triple point group was significantly higher than that of the other acupuncture group ( p < 0.05 ) .

2 . The effects of electroacupuncture on HPA axis in rats with depression model showed that the contents of ACTH and CORT in model group were significantly higher than those in blank group ( P0.01 ) .
Compared with the model group , the contents of ACTH and CORT in Jieyu group were significantly lower than those in model group ( P0.01 ) .

Conclusion

1 . Selecting the best acupoint group for acupuncture treatment of depression is Baihui + Shenmen + Taichong .

2 . The concentration of ACTH and CORT in the serum of rats can be adjusted by means of acupuncture and moxibustion , so the therapeutic mechanism of the depressed side of the acupoints may be related to the function of regulating the HPA axis .
【学位授予单位】：山西中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R285.5

【相似文献】