孕早期七氟烷母体麻醉对子代大鼠认知功能的影响及其机制

发布时间：2018-06-25 22:35

本文选题：七氟烷 + 母体麻醉　；参考：《中国临床药理学杂志》2017年20期

【摘要】：目的研究孕早期七氟烷母体麻醉对子代大鼠认知功能的影响,并对相关机制进行初步探讨。方法将27只7周龄SD雌性大鼠随机分为实验组-1(n=9)、实验组-2(n=9)和对照组(n=9)。于孕5~7 d时进行麻醉处理,实验组-1,实验组-2分别持续吸入1.8%七氟烷4,8 h,对照组仅吸入氧气8 h,氧流量均为2 L·min-1。每组均随机取6只母鼠所产子代SD大鼠,待出生后第30天开始进行Morris水迷宫系统测定其认知功能,用逆转录聚合酶链式反应(RT-PCR)及免疫组化法检测海马组织NR2B mRNA和HGN mRNA及蛋白表达情况。结果 Morris水迷宫系统测定结果显示,3组逃避潜伏期、穿越平台次数及在平台象限停留时间差异无统计学意义(P0.05)。实验组-1NR2BmRNA和HGN mRNA分别为0.79±0.11,0.66±0.08,实验组-2NR2BmRNA和HGN mRNA分别为0.77±0.09,0.65±0.07,对照组NR2B mRNA和HGN mRNA分别为0.78±0.08,0.68±0.07。3组NR2B mRNA和HGN mRNA表达结果差异无统计学意义(P0.05)。实验组-1、实验组-2及对照组NR2B蛋白阳性细胞的平均灰度值分别为99.68±17.68,97.62±18.63,101.38±15.52,HGN蛋白阳性细胞的平均灰度值分别为86.49±22.04,89.14±20.15,92.15±22.51,3组子代SD大鼠海马NR2B和HGN蛋白表达量差异无统计学意义(P0.05)。结论孕早期七氟烷母体麻醉对子代SD大鼠海马NR2B mRNA和HGN mRNA表达及NR2B蛋白表达无影响,是否与七氟烷麻醉剂量相关尚需进一步研究。
[Abstract]:Objective to study the effect of sevoflurane maternal anesthesia on the cognitive function of offspring rats in early pregnancy and to explore the related mechanism. Methods 27 7-week-old female SD rats were randomly divided into three groups: experimental group (n = 9), experimental group (n = 9) and control group (n = 9). Anesthetic treatment was performed on the 7th day of gestation. The experimental group (-1) and the experimental group (-2) inhaled 1.8% sevoflurane for 48 h, while the control group only inhaled oxygen for 8 h and the oxygen flow rate was 2 L min-1. In each group, 6 SD rats were randomly selected from their offspring, and their cognitive function was measured by Morris water maze system on the 30th day after birth. The expression of NR2B mRNA and HGN mRNA and protein in hippocampus were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Results the results of Morris water maze system showed that there was no significant difference in escape latency, crossing times and staying time in platform quadrant among the three groups (P0.05). The expression of NR2B mRNA and HGN mRNA were 0.79 卤0.110.66 卤0.08 in experimental group, 0.77 卤0.09 卤0.65 卤0.07 in experimental group, 0.78 卤0.080.68 卤0.07.3 in control group respectively (P0.05). The average gray values of NR2B protein positive cells in experimental group -2 and control group were 99.68 卤17.68, 97.62 卤18.63101.38 卤15.52 and 86.49 卤22.04 卤89.14 卤20.15 卤92.15 卤22.51, respectively. There was no significant difference in the expression of NR2B and HGN protein in hippocampus of SD rats (P0.05). Conclusion the maternal anesthesia of sevoflurane has no effect on the expression of NR2B mRNA, HGN mRNA and NR2B protein in hippocampus of SD rats in early pregnancy.
【作者单位】：海南现代妇婴医院麻醉科;
【分类号】：R614

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