电针对瑞芬太尼诱发大鼠痛觉过敏模型脊髓星形胶质细胞活化的影响

发布时间：2018-06-29 11:31

本文选题：瑞芬太尼 + 痛觉过敏　；参考：《中国新药杂志》2015年24期

【摘要】：目的:瑞芬太尼(RF)诱发的阿片源性痛觉过敏抑制了其在临床麻醉的使用。脊髓星形胶质细胞的活化可能与其相关。本实验探讨RF诱发痛觉过敏与星形胶质细胞活化的关系,同时研究电针治疗是否从机制上抑制痛敏的发生。方法:SD大鼠72只随机分为4组,每组均作切口痛模型:I组(切口痛对照组)尾静脉泵注生理盐水;R+I组(RF痛敏组)尾静脉泵注RF 0.8μg·kg-1;R+T组(电针治疗组)泵注RF前30 min开始电针治疗直至术毕;R+F组(假治疗组):泵注RF前以穴位电针刺激直至术毕。分别在术前24 h、RF输注后4,12,24和48 h测量各实验组大鼠的热刺激缩足潜伏期(PWTL)和机械刺激缩足阈(PWMT)。按不同时间点(4,24和48 h)处死实验大鼠,并取腰段脊髓测GFAP的浓度。结果:1行为学指标:RF痛敏组机械痛阈与热痛阈均高于切口痛对照组(P0.05),电针治疗组低于RF痛敏组(P0.05)。2标本GFAP表达:RF痛敏组明显高于切口痛对照组(P0.05),电针治疗组低于RF痛敏组(P0.05)。结论:实验证实了脊髓星形胶质细胞活化与瑞芬太尼诱导的痛觉过敏密切相关,电针治疗可以抑制其活化,从而预防瑞芬太尼导致的痛觉过敏,给临床的多模式镇痛提供理论依据。
[Abstract]:Objective: remifentanil (RF)-induced opioid hyperalgesia inhibits the use of anaesthesia. The activation of spinal astrocytes may be associated with it. This study was to investigate the relationship between RF induced hyperalgesia and astrocyte activation. Methods 72 Sprague-Dawley rats were randomly divided into 4 groups. Each group was given incision pain model: 1: 1 group (incision pain control group), caudal vein pump injection of normal saline Ri group (RF pain sensitive group) caudal vein pump injection RF 0.8 渭 g kg ~ (-1) R T group (electroacupuncture group) 30 min before pump injection of RF acupuncture treatment until the end of surgery R F Group (sham treatment group): before pump RF injection, acupoint electroacupuncture was used until the end of operation. The latent period of thermal stimulation (PWTL) and mechanical stimulation of foot contraction threshold (PWMT) were measured 24 and 48 hours after RF infusion at 24 hours before operation in each experimental group. The experimental rats were killed at different time points (4 h and 48 h), and the concentration of GFAP in lumbar spinal cord was measured. Results the mechanical pain threshold and thermal pain threshold of the 1: 1 behavioral index group were higher than those of the incision pain control group (P0.05), and the GFAP expression of GFAP in the electroacupuncture group was significantly higher than that in the incision pain control group (P0.05), and the expression of GFAP in the electroacupuncture group was significantly higher than that in the incision pain control group (P0.05), and that in the electroacupuncture group was lower than that in the RF pain sensitive group (P0.05). Conclusion: activation of astrocytes in spinal cord is closely related to remifentanil induced hyperalgesia. Electroacupuncture can inhibit the activation of remifentanil and prevent remifentanil induced hyperalgesia. To provide a theoretical basis for clinical multi-mode analgesia.
【作者单位】：安徽医科大学附属省立医院麻醉科;
【基金】：安徽省卫生厅中医药科研课题项目(2012zy45)
【分类号】：R614

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