丝胶对Fas介导的糖尿病大鼠胰岛β细胞凋亡的保护作用

发布时间：2018-07-06 19:25

本文选题：型糖尿病 + 丝胶　；参考：《中国老年学杂志》2015年18期

【摘要】：目的观察丝胶对2型糖尿病大鼠血糖、胰岛形态结构、胰岛细胞中胰岛素及胰岛β细胞细胞质死亡受体(Fas)表达的作用。方法48只雄性SD大鼠随机分为正常对照组、糖尿病模型组、二甲双胍组、丝胶治疗组,每组12只。采用腹腔注射链脲佐菌素(STZ)建立糖尿病动物模型,成模后,丝胶治疗组和二甲双胍组大鼠分别给予丝胶(2.4 g·kg-1·d-1)和二甲双胍(55.33 mg·kg-1·d-1)灌胃。给药4 w后禁食12 h,麻醉后取血,全自动生化分析仪检测大鼠空腹血糖(FPG);常规HE染色普通光镜下观察胰岛的形态结构和免疫组织化学染色法观察胰岛细胞中胰岛素表达情况;免疫组织化学染色法观察胰岛β细胞细胞质Fas蛋白的表达情况。结果 1与正常对照组相比,模型组大鼠FPG明显升高(P0.01);与模型组比较,丝胶治疗组和二甲双胍组FPG明显降低(P0.01);2与正常对照组相比,模型组胰岛萎缩、轮廓欠圆润、边缘不规则,胰岛细胞数量减少且分布稀疏而不均匀,胰岛素表达水平下降(P0.05);与模型组比较,丝胶治疗组和二甲双胍组大鼠胰岛细胞数量增多,分布也比较致密,胰岛素表达水平上升(P0.05);3与正常对照组相比,模型组Fas蛋白的表达明显降低(P0.05),与模型组比较,丝胶治疗组和二甲双胍组Fas蛋白的表达明显升高(P0.05)。以上指标丝胶治疗组和二甲双胍组比较均无明显差异(P0.05)。结论丝胶对2型糖尿病具有治疗作用,其机制可能与抑制Fas介导的胰岛β细胞凋亡有关。
[Abstract]:Objective to observe the effects of sericin on blood glucose, islet morphology, insulin and 尾 cytoplasmic death receptor (FAS) expression in islet cells of type 2 diabetic rats. Methods Forty-eight male SD rats were randomly divided into normal control group, diabetic model group, metformin group and sericin treatment group with 12 rats in each group. The diabetic model was established by intraperitoneal injection of streptozotocin (STZ). The rats in sericin treatment group and metformin group were given sericin (2.4g kg-1 d-1) and metformin (55.33mg kg-1 d-1) respectively. Fasting for 12 hours after 4 weeks of administration, blood samples were taken after anesthesia, fasting blood glucose (FPG) in rats was detected by automatic biochemical analyzer, morphological structure of islets and insulin expression in islet cells were observed by routine HE staining and immunohistochemical staining. The expression of FAS protein in islet 尾 cytoplasm was observed by immunohistochemical staining. Results 1 compared with the normal control group, the FPG of the model group was significantly higher (P0.01), and the FPG of sericin treatment group and metformin group was significantly lower than that of the normal control group (P0.01), the islet atrophy of the model group was less than that of the normal control group, the contour of the model group was not smooth, and the edge of the model group was irregular. Compared with the model group, the number and distribution of islet cells in sericin treatment group and metformin group were increased, and the distribution of islet cells was more dense. Compared with the normal control group, the expression of FAS protein in the model group was significantly decreased (P0.05). Compared with the model group, the expression of FAS protein in sericin treatment group and metformin group was significantly increased (P0.05). There was no significant difference between the sericin treatment group and metformin group (P0.05). Conclusion sericin has therapeutic effect on type 2 diabetes mellitus, and its mechanism may be related to inhibition of FAS mediated apoptosis of islet 尾 cells.
【作者单位】：承德医学院;
【基金】：河北省自然科学基金项目(H2013406096)
【分类号】：R285.5

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