基于甘满生痰的肝癖致病机制的实验研究

发布时间：2018-07-08 11:49

本文选题：高糖饮食 + 非酒精性脂肪肝　；参考：《时珍国医国药》2017年01期

【摘要】：目的比较过食肥甘、过食肥甘同时过逸,导致生痰致瘀、痰瘀互结,而发肝癖的特点,为临床基于甘满生痰防治肝癖的思路提供参考。方法以高糖饲料饲养模拟过食肥甘、致痰湿内生、痰瘀互结而生肝癖;以丙基硫氧嘧啶(PTU)抑制代谢率、减少消耗模拟过逸。观察其对血脂、肝脏脂肪变的影响,以探讨甘满生痰对肝癖的致病机制,为饮食防治非酒精性脂肪肝(NAFLD)提供依据。方法正常雄性SD大鼠30只,按体重随机分成高糖1组、高糖2组和对照组。高糖1组饲以高糖低代谢饲料,高糖2组饲以高糖饲料,对照组饲以正常饲料。16周后,禁食不禁水12h,麻醉后取血清检测肝功能、血脂,取肝脏组织进行油红O及HE染色。结果甘油三酯(TG):高糖2组高于其他两组,胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C):高糖1组高于其他两组,谷丙转氨酶(ALT):高糖1组明显高于其他两组,谷草转氨酶(AST)、γ-谷氨酰转移酶(γ-GT):高糖1组高于其他两组,脂肪肝程度:高糖两组均有脂肪变性,2组较1组严重,对照组为阴性。结论高糖饮食能导致肝脏一定程度的脂肪变性,并能导致血脂、肝功能异常;过食肥甘是酿成痰湿、郁久化瘀、痰瘀互结损伤肝脏而致肝癖的重要原因。
[Abstract]:Objective to compare the characteristics of febrile Gan and excessive fatness at the same time, resulting in phlegm and blood stasis, and to provide a reference for clinical treatment of liver fetid based on Ganmansheng phlegm. Methods the rats were fed with high sugar diet to induce phlegm dampness endogenesis phlegm and blood stasis to produce liver fetid while propyl thiouracil (PTU) inhibited metabolic rate and reduced consumption of simulated excessive escape. In order to explore the pathogenic mechanism of Ganmansheng phlegm on lipids and liver steatosis, it can provide basis for diet prevention and treatment of non-alcoholic fatty liver (NAFLD). Methods 30 normal male SD rats were randomly divided into three groups according to their body weight: high glucose group, high glucose group 2 and control group. Group 1 was fed with high sugar and low metabolism diet, group 2 with high sugar and control group with normal diet for 16 weeks. After fasting for 12 hours, serum samples were taken to detect liver function, blood lipid and liver tissues for oil red O and HE staining. Results triglyceride (TG): high glucose group 2 was higher than the other two groups, cholesterol (TC), low density lipoprotein cholesterol (LDL-C): high glucose group 1 was higher than the other two groups, alt: high glucose group 1 was significantly higher than the other two groups. Aspartate aminotransferase (AST), 纬 -glutamyl transferase (纬 -GT): high glucose group 1 was higher than the other two groups, fatty liver degree: the fatty liver degree of both groups were more serious than that of the first group, but the control group was negative. Conclusion High glucose diet can lead to a certain degree of fatty degeneration of liver, and lead to abnormal blood lipid and liver function, too much fat and sweet food is an important cause of liver damage caused by phlegm dampness, depression and blood stasis, phlegm and blood stasis.
【作者单位】：福建中医药大学;福建中医药大学附属人民医院;
【基金】：国家自然科学基金(No.81173179;No.81473550;No.81403329) 福建省科技社会发展重点项目(No.2012Y0037) 福建省卫生厅中医药科研重点研究室基金(No.wztn 201304) 福建中医药大学重点学科专项基金(No.X2014037)
【分类号】：R285.5

【相似文献】