中枢疲劳恢复期大鼠海马GDNF、GFRα-1mRNA及其蛋白表达的动态变化特征

发布时间：2018-07-10 16:01

本文选题：中枢疲劳 + 运动性疲劳　；参考：《山东体育学院学报》2015年02期

【摘要】：目的:探讨大鼠海马组织胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)、GDNF家族受体α-1信使RNA(GDNF?family receptorα-1mRNA,GFRα-1mRNA)及其蛋白表达在中枢疲劳后恢复期不同时相的动态变化特征。方法:雄性2月龄SD大鼠32只,随机分为对照组(C组,n=8)、实验组(E组,n=24);E组进行7周疲劳模型运动后,又随机分为运动后即刻组(E0组,n=8)、恢复期12 h组(E1组,n=8)、恢复期24 h组(E2组,n=8)。C组正常笼养,E组进行7周递增负荷跑台训练。经7周疲劳模型运动后即刻,C组和E0组腹腔注射10%水合氯醛糖浆(0.3m L/100g)麻醉后取海马组织,用于测定5-羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、GDNF、GFRα-1mRNA及其蛋白表达;E1组和E2组分别在恢复期12 h、24 h做上述同样取材和测试。结果:1)大鼠经7周递增负荷跑台训练后即刻,海马组织5-HT升高(P0.01),DA下降(P0.01),DA/5-HT下降(P0.05)。2)运动后即刻海马组织GDNF、GFRα-1mRNA相对表达率和GDNF、GFRα-1平均蛋白表达水平升高;恢复期12 h高于C组和E0组(P0.01);恢复期24 h降低并低于E1组(P0.01)。结论:1)经7周疲劳模型运动后大鼠已经出现中枢疲劳。2)运动性中枢疲劳后大鼠海马组织GDNF、GFRα-1mRNA及其蛋白表达水平上升,提示GDNF和GFRα-1可能参与了中枢疲劳恢复的神经生物学调控过程。
[Abstract]:Aim: to investigate the dynamic changes of GDNF family receptor 伪 -1mRNA-GFR 伪 -1mRNA and its protein expression in the recovery phase after central fatigue in rat hippocampal tissue with glial cell-derived neurotrophic factor (glial cell line-derived neurotrophic factor-GDNF). Methods: Thirty-two male 2-month-old SD rats were randomly divided into control group (group C, n = 8) and experimental group (group E, n = 24) after 7 weeks of fatigue exercise. They were randomly divided into three groups: immediate exercise group (E0 group), 12 h recovery group (E1 group) and 24 h recovery period group (E2 group). Group C was given 7 weeks incremental load treadmill training. After 7 weeks of fatigue exercise, hippocampal tissue was taken out in group C and group E0 by intraperitoneal injection of 10% chloral hydrate syrup (0.3m L / 100g). To determine the expression of 5-hydroxytryptamine-5-HT, dopamine (DA), GDNF-GFR 伪 -1 mRNA and their protein expression in E1 group and E2 group, the same materials were obtained and tested at 12 h and 24 h after recovery, respectively. Results (1) immediately after 7 weeks of increasing load treadmill training, the hippocampal 5-HT increased (P0.01) DA decreased (P0.01) and DA-5-HT decreased (P0.05). Immediately after exercise, the relative expression rate of GDNFGFR 伪 -1 mRNA and the average expression level of GDNFGFR 伪 -1 protein in hippocampal tissue were increased. The recovery period was 12 h higher than that of C group and E0 group (P0.01), and 24 h of convalescence period was lower than that of E1 group (P0.01). Conclusion (1) after 7 weeks of exercise, the rats have developed central fatigue. 2) after exercise induced fatigue, the expression of GDNFF GFR 伪 -1 mRNA and its protein in hippocampal tissue of rats increased, suggesting that GDNF and GFR 伪 -1 may be involved in the neurobiological regulation of central fatigue recovery.
【作者单位】：山东体育学院;山东省微山县实验中学;山东省体育科学研究中心;
【基金】：山东省自然科学基金(ZR2009CQ032)
【分类号】：G804.7

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