咪达唑仑对离体胎鼠神经细胞的影响研究
发布时间：2018-07-16 15:17
【摘要】：实验目的： 咪达唑仑是一种经典的短效苯二氮卓类药物,临床上通常用于术前镇静。为明确咪达唑仑对中枢神经系统中氨基酸类神经递质,单胺类神经递质及ATP酶跨膜信号转导系统、cAMP信号转导通路和NO-cGMP信号转导通路的影响,探讨其发挥镇静作用的主要靶位,及参与麻醉的可能路径。 实验方法： 本实验以胎鼠为实验对象,将90只胎鼠分为3组,每组设3个平行。根据在体实验测得咪达唑仑在大鼠血浆中的浓度范围为5-60μg/ml,本实验选取3个浓度组,低剂量组3.41×10-3ug／ml,中剂量组5μg/ml,高剂量组60μg/ml采用直接细胞给药的方式,于051015202530456090120min时采样,采用质谱检测单胺类神经递质,氨基酸类神经递质,酶标仪法测量信号转导通路中ATP酶的活性,NO及NOS的含量,用ELISA测定cGMP和cAMP含量。 实验结果： 1、不同浓度的咪达唑仑能减少神经元兴奋性神经递质的含量,增加抑制性神经递质的含量。 2、不同浓度的眯达唑仑对儿茶酚胺类神经递质和五羟色胺的作用趋势是先升高后降低,对五羟吲哚乙酸的作用趋势是先降低后升高。 3、不同浓度的咪达唑仑对ATP酶的影响是显著降低,对NO和NOS的影响是显著降低,对cAMP和cGMP的影响是先降低后升高再降低。 结论： 1咪达唑仑能降低兴奋性神经递质和单胺类神经递质的含量。 2咪达唑仑对ATP酶,cAMP和cGMP信号通路的作用是抑制性的。 3咪达唑仑的镇静机制可能与抑制兴奋性神经递质,ATP酶,cAMP和cGMP信号通路有关。
[Abstract]:Objective: midazolam is a classic short-acting benzodiazepines and is commonly used for preoperative sedation. To elucidate the effects of midazolam on amino acid neurotransmitters, monoamine neurotransmitters, ATPase transmembrane signal transduction pathway and NO-cGMP signal transduction pathway in the central nervous system, and to explore the main targets of midazolam for sedation. And possible pathways involved in anesthesia. Methods: 90 fetal mice were divided into 3 groups with 3 parallel in each group. The concentration of midazolam in rat plasma was measured in vivo in the range of 5-60 渭 g / ml. In this experiment, three concentration groups were selected: low dose group (3.41 脳 10 ~ (-3) 渭 g / ml), medium dose group (5 渭 g / ml) and high dose group (60 渭 g/ml). Monoamine neurotransmitters and amino acid neurotransmitters were detected by mass spectrometry. The activity of ATPase no and NOS in signal transduction pathway were measured by enzyme label method. CGMP and camp were measured by Elisa. Results: 1. Midazolam at different concentrations can reduce the content of excitatory neurotransmitters in neurons. Increasing the content of inhibitory neurotransmitters. (2) the effect of different concentrations of midazolam on catecholamine neurotransmitters and serotonin increased first and then decreased. The effect of midazolam at different concentrations on ATPase, no and NOS was significantly decreased, and on camp and cGMP decreased first and then decreased. Conclusion: 1 midazolam can reduce the contents of excitatory neurotransmitters and monoamine neurotransmitters. 2 midazolam inhibits the ATP enzyme camp and cGMP signaling pathway. The sedative mechanism of zolam may be related to the inhibition of camp and cGMP signaling pathway.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：S859.79
[Abstract]:Objective: midazolam is a classic short-acting benzodiazepines and is commonly used for preoperative sedation. To elucidate the effects of midazolam on amino acid neurotransmitters, monoamine neurotransmitters, ATPase transmembrane signal transduction pathway and NO-cGMP signal transduction pathway in the central nervous system, and to explore the main targets of midazolam for sedation. And possible pathways involved in anesthesia. Methods: 90 fetal mice were divided into 3 groups with 3 parallel in each group. The concentration of midazolam in rat plasma was measured in vivo in the range of 5-60 渭 g / ml. In this experiment, three concentration groups were selected: low dose group (3.41 脳 10 ~ (-3) 渭 g / ml), medium dose group (5 渭 g / ml) and high dose group (60 渭 g/ml). Monoamine neurotransmitters and amino acid neurotransmitters were detected by mass spectrometry. The activity of ATPase no and NOS in signal transduction pathway were measured by enzyme label method. CGMP and camp were measured by Elisa. Results: 1. Midazolam at different concentrations can reduce the content of excitatory neurotransmitters in neurons. Increasing the content of inhibitory neurotransmitters. (2) the effect of different concentrations of midazolam on catecholamine neurotransmitters and serotonin increased first and then decreased. The effect of midazolam at different concentrations on ATPase, no and NOS was significantly decreased, and on camp and cGMP decreased first and then decreased. Conclusion: 1 midazolam can reduce the contents of excitatory neurotransmitters and monoamine neurotransmitters. 2 midazolam inhibits the ATP enzyme camp and cGMP signaling pathway. The sedative mechanism of zolam may be related to the inhibition of camp and cGMP signaling pathway.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：S859.79
【参考文献】
相关期刊论文 前10条
1 刘秀华，李涛，乔桂林，，马莺;二甲苯胺噻唑对绵羊血液及脑脊液中去甲肾上腺素的影响[J];东北农业大学学报;1994年02期
2 孙绪德;高昌俊;张惠;张贵和;吕苗苗;柴伟;徐礼鲜;;氯胺酮麻醉下犬丘脑内神经递质的变化[J];第四军医大学学报;2008年22期
3 马晓旭,戴体俊,曾因明;NO/cGMP信号转导系统与氯胺酮麻醉[J];国外医学.麻醉学与复苏分册;2004年04期
4 成同怡;吴本s
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