药物及酒精对实验性糖尿病大鼠肠系膜微循环的影响

发布时间：2018-07-17 19:45

【摘要】：目的：研究去甲肾上腺素（Norepinephrine、NE)、硝普钠（Sodium nitroprusside、SNP)及酒精（Alcohol）对麻醉实验性糖尿病大鼠肠系膜微动、静脉血管反应性的影响。方法:正常雄性SD大鼠（220～250g）78只，诱导实验性糖尿病大鼠（STZ）78只。采用BI-2000医学图像分析系统，采集麻醉大鼠肠系膜微循环显微图像，比较观察局部滴加不同浓度NE(10-12～10-4mol/L)、SNP(10-10～10-4mol)、酒精（0.025%～3.2%）及灌胃不同浓度酒精（0.375%～48%、5min）对实验性糖尿病大鼠肠系膜微血管直径的影响，并且观察酚妥拉明（Phentolamine）对NE收缩血管作用的影响及左旋硝基精氨酸甲酯（L-NAME）对SNP舒张血管的影响。 1.第一部分，局部分别滴加不同浓度NE(10-12～10-4mol/L)于对照组及实验性糖尿病大鼠肠系膜微血管，观察NE对大鼠肠系膜微动、静脉血管反应性的影响；酚妥拉明孵育1min后，NE对大鼠肠系膜微动、静脉血管收缩作用的影响。 2.第二部分，局部分别滴加不同浓度SNP(10-10～10-4mol/L)于对照组及实验性糖尿病大鼠肠系膜微血管，观察SNP对大鼠肠系膜微动、静脉血管反应性的影响；L-NAME孵育1min后，SNP对大鼠肠系膜微动、静脉血管舒张作用的影响。 3.第三部分，局部滴加不同浓度酒精（0.025%～3.2%）及灌胃不同浓度酒精（0.375％～48％）对大鼠肠系膜微动、静脉血管反应性的影响。结果： 1、第一部分，NE(10-12～10-4mol/L)浓度依赖性收缩大鼠肠系膜微动、静脉直径。NE诱导微动、静脉收缩的浓度-效应曲线的EC50（收缩达最大收缩50%时的药物浓度）分别为(4.14±0.35)×10-9mol/L、(2.95±0.48)×10-8mol/L。与对照组相比较，实验性糖尿病大鼠对NE的反应性显著减弱（P 0.05），NE诱导微动、静脉收缩EC50分别为(5.39±0.29)×10-8mol/L、(4.78±0.39)×10-8mol/L。酚妥拉明孵育后，NE对对照组大鼠肠系膜微血管的收缩作用明显强于STZ组。 2、第二部分，SNP(10-10～10-4mol/L)浓度依赖性舒张大鼠肠系膜微动、静脉直径，SNP诱导微动、静脉舒张的EC50分别为（2.44±0.32）×10-8mol/L、(5.85±0.40)×10-8mol/L。与对照组相比较，，实验性糖尿病大鼠肠系膜微血管对SNP的反应性明显减弱（P 0.05），SNP诱导微动、静脉舒张EC50分别为(6.66±0.43)×10-8mol/L、(8.78±0.59)×10-8mol/L。L-NAME孵育后，SNP对对照组大鼠肠系膜微血管的舒张率明显高于STZ组。 3、第三部分，酒精浓度依赖性舒张大鼠肠系膜微动、静脉直径（P 0.05），局部滴加酒精诱导微动、静脉舒张EC50分别为（0.13±0.02）%、（0.17±0.02）%，灌胃酒精诱导微动、静脉舒张的EC50分别为（2.81±0.22）%、（2.85±0.21）%。与对照组相比较，局部滴加酒精诱导微动、静脉舒张的EC50分别为(0.25±0.03)%、（0.32±0.03）%，灌胃酒精诱导微动、静脉舒张的浓度-效应曲线的EC50分别为(2.79±0.23)%、（3.25±0.23）%，实验性糖尿病大鼠肠系膜微血管对酒精的反应性明显减弱。结论： 1. STZ诱导实验性糖尿病大鼠肠系膜微循环对NE的反应性显著减弱。2.STZ诱导实验性糖尿病大鼠肠系膜微循环对SNP的反应性明显减弱。3. STZ诱导实验性糖尿病大鼠肠系膜微循环对酒精的反应性明显减弱。
[Abstract]:Objective: To study the effect of Norepinephrine (NE), sodium nitroprusside (Sodium nitroprusside, SNP) and alcohol (Alcohol) on the mesenteric micromovement and venous reactivity of the experimental diabetic rats.
Methods: 78 normal male SD rats (220 ~ 250g) were used to induce 78 rats of experimental diabetic rats (STZ). The microcirculation images of mesenteric microcirculation in anesthetized rats were collected by BI-2000 medical image analysis system. The different concentrations of NE (10-12 ~ 10-4mol/L), SNP (10-10 to 10-4mol), alcohol (0.025% to 3.2%) and different concentration of alcohol in the gavage were observed. The effect of sperm (0.375% ~ 48%, 5min) on the mesenteric microvascular diameter of experimental diabetic rats and the effect of phentolamine (Phentolamine) on the vasoconstrictor effect of NE and the effect of L-nitroarginine methyl ester (L-NAME) on the vasodilatation of SNP were observed.
1. in the first part, the mesenteric microvessels of the control group and experimental diabetic rats were partially added with different concentrations of NE (10-12 ~ 10-4mol/L) in the control group and experimental diabetic rats. The effects of NE on the mesenteric micromovement and venous reactivity of the rats were observed. After the incubation of phentolamine for 1min, the effect of NE on the mesenteric micromovement and venous vasoconstriction in rats.
2. in the second part, the mesenteric microvessels of the control group and experimental diabetic rats were separately added with different concentrations of SNP (10-10 ~ 10-4mol/L) in the control group and experimental diabetic rats. The effects of SNP on the mesenteric micromovement and venous reactivity of the rats were observed. After L-NAME incubation, the effect of SNP on the mesenteric micromovement and the vasodilatation of the rats was induced by SNP.
3. in the third part, the effects of different concentrations of alcohol (0.025% ~ 3.2%) and alcohol (0.375% ~ 48%) on the reactivity of the mesentery and venous reactivity of the rat mesentery (0.375% ~ 48%) were added.
Result:
1, the first part, NE (10-12 ~ 10-4mol/L) concentration dependent contraction rats mesenteric micromovement, venous diameter.NE induced fretting, the concentration effect curve of venous contraction of EC50 (the maximum contraction of 50% when the contraction of 50%) was (4.14 + 0.35) x 10-9mol/L, (2.95 + 0.48) x 10-8mol/L. compared with the control group, experimental diabetic rats N The reactivity of E was significantly decreased (P 0.05), NE induced micromovement and venous systolic EC50 was (5.39 + 0.29) x 10-8mol/L respectively, and (4.78 + 0.39) x 10-8mol/L. phentolamine incubated by NE. The contractile effect of NE on the mesenteric microvessels of the control group was significantly stronger than that of the STZ group.
The 2, second part, SNP (10-10 ~ 10-4mol/L) concentration dependent diastolic rat mesenteric micromovement, venous diameter, SNP induced micromovement, the diastolic EC50 was (2.44 + 0.32) x 10-8mol/L and (5.85 + 0.40) x 10-8mol/L. compared with the control group. The reactivity of mesenteric microvessels to SNP in experimental diabetic rats decreased significantly (P 0.05), SNP induction The diastolic diastolic rate of EC50 was (6.66 + 0.43) x 10-8mol/L and (8.78 + 0.59) x 10-8mol/L.L-NAME respectively. The diastolic rate of the mesenteric microvessel in the control group was significantly higher than that of the STZ group.
The 3 and third parts, the alcohol concentration dependent diastolic rat mesenteric micromovement, the diameter of the vein (P 0.05), the local dripping of alcohol induced micromovement, and the diastolic EC50 were (0.13 + 0.02)%, (0.17 + 0.02)%, the gavage alcohol induced micromovement, the EC50 of the diastolic diastole was (2.81 + 0.22)%, and (2.85 + 0.21)% respectively. Compared with the control group, the local drop of alcohol induced micromanipulation was compared with the control group. The diastolic EC50 was (0.25 + 0.03)% and (0.32 + 0.03)% respectively. The alcohol induced micromovement was induced by intragastric alcohol, and the EC50 of the concentration effect curve of venous diastole was (2.79 + 0.23)% and (3.25 + 0.23)% respectively. The reactivity of mesenteric microvessels to alcohol in experimental diabetic rats decreased significantly.
Conclusion:
1. STZ induced diabetic rat mesenteric microcirculation significantly reduced the reactivity of mesenteric microcirculation to NE induced by.2.STZ induced diabetic rats, the reactivity of mesenteric microcirculation to SNP was significantly reduced by.3. STZ induced diabetic rats, and the reactivity of mesenteric microcirculation to alcohol was significantly weakened in experimental diabetic rats.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R587.1

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