裂殖壶藻藻油DHA对高脂饮食诱导肥胖小鼠的影响

发布时间：2018-08-03 18:25

【摘要】：【目的】肥胖症是一种慢性代谢类疾病,具有较高的发病率和高危后果。研究表明,n-3多不饱和脂肪酸(n-3 Polyunsaturated fatty acids,n-3 PUFAs),特别是二十二碳六烯酸(Docosahexaenoic acid,DHA)对与肥胖症相关疾病有较好的防治效果,对体内脂质代谢有重要的调节作用。探讨裂殖壶藻(Schizochytrium sp.)藻油DHA对高脂饮食诱导肥胖小鼠体重、脂肪组织重量、血脂、肝和脂肪组织病理形态和脂质代谢相关基因表达的影响。【方法】通过高脂饮食建立小鼠肥胖模型,以体重增幅15%为标准分出肥胖小鼠。试验共分五组:(1)低脂对照组;(2)高脂模型组;(3)高脂+低剂量藻油组(50 mg DHA/kg);(4)高脂+中剂量藻油组(100 mg DHA/kg);(5)高脂+高剂量藻油组(200 mg DHA/kg)。其中,藻油处理组灌服相应剂量藻油,低脂对照组和高脂模型组灌胃同等体积玉米油。处理9周后,腹腔麻醉,摘眼球取血并分离血清,测血清中甘油三酯、胆固醇和高密度脂蛋白含量;之后处死小鼠,分离附睾、肾周和肠系膜脂肪组织及肝脏,称湿重;附睾脂肪和肝组织切片进行HE染色,观察病理变化情况;利用RT-PCR检测附睾脂肪组织中激素敏感脂酶(Hormone sensitive lipase,HSL)基因的m RNA表达情况。【结果】藻油处理组小鼠体重没有显著下降,但是腹部脂肪重量显著降低、脂肪细胞体积明显小于高脂模型组;同时血清中甘油三酯、胆固醇含量显著降低,肝组织异位脂肪堆积明显减少;脂肪组织中HSL基因的表达水平显著提高。【结论】裂殖壶藻藻油DHA处理能显著降低高脂饮食导致的小鼠腹部脂肪积累并改善血脂,可能有利于肥胖症的防治。
[Abstract]:[Objective] obesity is a chronic metabolic disease with high incidence and high risk consequences. Studies have shown that n-3 polyunsaturated fatty acids (n-3 Polyunsaturated fatty acids, n-3 PUFAs), especially twenty-two carbon six enoic acid (Docosahexaenoic acid, DHA), have good control effect on obesity related diseases and liposomes in vivo. The effects of Schizochytrium sp. on the body weight, fat tissue weight, lipid, liver and fatty tissue, and the expression of lipid metabolism related genes in obese mice induced by high fat diet were investigated. [Methods] the obesity model of mice was established by high fat diet, with 15% weight gain as the standard score. The obese mice were divided into five groups: (1) low fat control group; (2) high fat model group; (3) high fat + low dose algal oil group (50 mg DHA/kg); (4) high fat + medium dose algal oil group (100 mg DHA/kg); (5) high fat + high dose algal oil group (200 mg DHA/kg). After 9 weeks of treatment, the corn oil was treated with intraperitoneal anesthesia, picked up the blood and separated the serum, and measured the content of triglyceride, cholesterol and high density lipoprotein in the serum, then killed the mice, separated epididymis, perirenal and mesenteric adipose tissue and liver, and called wet weight; epididymal fat and liver tissue section were stained with HE to observe the pathological changes; use RT-PCR The expression of M RNA in the Hormone sensitive lipase (HSL) gene in the epididymal adipose tissue was detected. [results] the weight of the mice in the algal oil treatment group was not significantly decreased, but the weight of the abdominal fat decreased significantly and the fat cell volume was significantly smaller than the high fat model group, and the serum triglyceride and cholesterol content decreased significantly. The accumulation of ectopic fat in liver tissue was significantly reduced, and the expression level of HSL gene in adipose tissue was significantly increased. [Conclusion] DHA treatment of alga alga oil can significantly reduce fat accumulation in abdominal fat induced by high fat diet and improve blood lipid, which may be beneficial to the prevention and treatment of obesity.
【作者单位】：山东省农业科学院山东省农作物种质资源中心;山东省农业科学院生物技术研究中心;山东大学生命科学学院;
【基金】：山东省农业科学院青年科研基金(No.2015YQN32) 山东省现代农业产业技术体系建设专项资金(No.SDAIT-26-09) 国家国际科技合作专项项目(No.2012DFA30450)~~
【分类号】：R589.2

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