益肾宁心中药对环境污染物影响卵巢功能作用的研究

发布时间：2018-08-12 17:45

【摘要】：目的:探究益肾宁心中药对环境污染物去氧乙烯基环乙烯(4-vinylcyclohexene diepoxide,VCD)所致卵巢功能不全模型大鼠的疗效。方法:40只21日龄离乳大鼠,随机分为佐剂对照组、模型组、坤泰胶囊低剂量组、坤泰胶囊中剂量组、坤泰胶囊高剂量组,每组8只。除佐剂对照组外的四组均给予15天腹腔注射浓度为80mg/kg VCD溶液(VCD溶于芝麻油中)2.5ml/kg · d,佐剂对照组腹腔注射15天同等剂量芝麻油2.5ml/kg·d。于第一次腹腔注射VCD时,坤泰胶囊低、中、高剂量组分别灌胃给药45天。待VCD注射完后70±5天,根据阴道涂片情况,将持续处于动情间期或持续动情周期紊乱的大鼠麻醉取材,进行检测大鼠血清促卵泡生成素(Follicle-Stimulating Hormone,FSH)、促黄体生成素(leuteinizing hormone,.LH)、雌二醇(estradiol,E2)、抗苗勒管激素(anti-Mullerian hormone,AMH)等;取出卵巢、子宫进行形态学观察。结果:①与佐剂对照组相比,模型组大鼠血清FSH明显增高且AMH、E2明显降低(P均0.05),LH有所增高(P0.05);经坤泰胶囊干预后,FSH水平显著降低(P0.05),AMH水平明显增加(P0.05);②模型组大鼠卵巢湿重较佐剂对照组减轻(P0.05),坤泰胶囊干预后,坤泰胶囊低剂量组卵巢湿重增加明显(P0.05);③模型组大鼠储备卵泡及成熟卵泡计数明显减少(P均0.05),坤泰胶囊干预后,储备卵泡及成熟卵泡计数明显增加(P均0.05);④光镜下,模型组子宫内膜变薄,管壁子宫腺体数量明显减少。经坤泰胶囊干预后,内膜有所增厚,腺体数量明显增加。结论:1、成功构建了由VCD所致大鼠卵巢功能不全的模型,具体表现为:血清性激素水平、卵巢湿重、卵泡数量、卵巢子宫形态均发生了改变;2、坤泰胶囊可对上述改变有明显改善调节作用。
[Abstract]:Aim: to investigate the effect of Yishen Ningxin traditional Chinese medicine on ovarian dysfunction induced by environmental pollutant 4-vinylcyclohexene diepoxide (DEVCD) in rats. Methods Forty 21-day-old weaned rats were randomly divided into adjuvant control group, model group, low dose group, middle dose group and high dose group with 8 rats in each group. The four groups except the adjuvant control group were given intraperitoneal injection of 80mg/kg VCD solution (VCD soluble in sesame oil) 2.5ml/kg d for 15 days, while the adjuvant control group received the same dose of sesame oil 2.5ml/kg d for 15 days. At the first intraperitoneal injection of VCD, Kuntai capsule was given orally for 45 days in low, medium and high dose groups. After 70 卤5 days of VCD injection, according to the vaginal smear, anesthetized rats with continuous estrous interphase or continuous estrous cycle disorder were anesthetized. The serum levels of Follicle-Stimulating, leuteinizing, estradiolol E 2 and anti-Mullerian hormone were detected, and the ovary was removed and the uterus was observed. Results compared with the adjuvant control group, In model group, serum FSH was significantly increased and AMH- E _ 2 was significantly decreased (P < 0.05), FSH level was significantly decreased (P0.05) after intervention of Kun Tai capsule (P0.05) and the wet weight of ovary was significantly decreased in model group (P0.05) than that in adjuvant control group (P0.05), and that in Kun Tai capsule was significantly lower than that in adjuvant control group (P0.05), and that in Kun Tai capsule group was significantly lower than that in adjuvant control group (P0.05). The wet weight of ovary increased significantly (P0.05) in the low dose group of Kun Tai capsule (P0.05). The number of reserve follicles and mature follicles in the model group decreased significantly (P 0.05). After the intervention of Kun Tai capsule, the number of reserve follicles and mature follicles increased significantly (all P 0.05) under light microscope. In the model group, the endometrium became thinner and the number of uterine glands on the wall decreased significantly. After the intervention of Kuntai capsule, the intima was thickened and the number of glands was obviously increased. Conclusion: 1, the rat model of ovarian dysfunction induced by VCD was successfully constructed. The specific manifestations were: serum sex hormone level, wet weight of ovary, number of follicles. The uterine morphology of the ovary has been changed and Kun Tai capsule can obviously improve and regulate the above changes.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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