氯胺酮对人肺癌A549细胞生长的影响及相关机制研究

发布时间：2018-08-17 15:18

【摘要】：目的 :探讨麻醉类药物氯胺酮和吗啡对人肺癌A549细胞生长的影响及相关作用机制。方法:体外培养肺癌细胞系A549,细胞培养24 h后,随机分为3组:对照组(NC组,加0.9%Na Cl处理)、氯胺酮组(K1、K2、K3组,分别加氯胺酮100、200、400μg/m L处理)和吗啡组(M1、M2、M3组,分别加吗啡25、50、100μg/m L处理)。持续作用24 h后,Transwell小室培养体系测定肿瘤细胞的迁移率;双荧光素酶报告基因法测定NF-κB转录活性;48 h后MTT法测定细胞增殖抑制率;Western blot技术测定NF-κB P65的蛋白表达;QPCR技术检测细胞内IL-1β和COX-2的m RNA表达。结果:1与NC组比较,M1、M2组表现为促进A549细胞迁移(P0.05),M3组作用不明显;K1、K2、K3组表现为抑制A549细胞迁移(P0.05);2与NC组比较,M1、M2组表现为促进A549细胞增殖(P0.05),M3组作用不明显;K2、K3组表现为抑制A549细胞增殖(P0.05),K1组不明显;3与NC组比较,M1、M2、M3组表现为促进IL-1β、COX-2的m RNA表达(P0.05);K2、K3组表现为抑制IL-1β、COX-2的m RNA表达(P0.05),K1组不明显;4与NC组比较,M1、M2、M3组表现为促进P65的转录和蛋白表达(P0.05),K1、K2、K3组表现为抑制P65的转录和蛋白表达(P0.05)。结论:与阿片类药物不同,氯胺酮通过NF-κB信号通路及其下游因子IL-1β和COX-2抑制人肺腺癌细胞A549的迁移和增殖,可能更适用于肺癌患者的术后镇痛。
[Abstract]:Aim: to investigate the effect of ketamine and morphine on the growth of human lung cancer cell line A549. Methods: lung cancer cell line A549 was cultured in vitro for 24 hours. After 24 h culture, the cells were randomly divided into three groups: control group (NC group, treated with 0.9%Na Cl), ketamine group (K1K2K2K3 group, treated with ketamine 100200400 渭 g / mL) and morphine group (M1m2M3 group, treated with morphine 2550100 渭 g / mL, respectively). The migration rate of tumor cells was measured in transwell chamber culture system after 24 hours of continuous treatment. Double luciferase reporter gene assay for NF- 魏 B transcriptional activity 48 h later, the inhibitory rate of cell proliferation was determined by MTT assay. Protein expression of NF- 魏 B p65 was detected by Western blot. The expression of IL-1 尾 and m RNA in cells was detected by QPCR. Results compared with NC group, the effect of M1M 2 group was to promote the migration of A549 cells (P0.05) the effect of M3 group was to inhibit the migration of A549 cells (P0.05). Compared with the NC group, the M1M 2 group was able to promote the proliferation of A549 cells (P0.05), the effect of M3 group was not obvious, and the K2K3 group showed no obvious inhibitory effect on the proliferation of A549 cells. Proliferation of A549 cells (P0.05) the expression of m RNA of IL-1 尾 -COX-2 in M1M2M3 group was significantly higher than that in NC group (P0.05). (P0.05) the expression of m RNA of IL-1 尾 -COX-2 was inhibited in K2K3 group (P0.05). 4 the expression of p65 transcription and protein in M1M2M3 group was not obvious compared with that in NC group (P0.05). (P 0.05) the expression of p65 transcription and protein was inhibited in K1 K2K3 group (P0.05). Conclusion: ketamine inhibits the migration and proliferation of human lung adenocarcinoma cell line A549 through NF- 魏 B signaling pathway and its downstream factors IL-1 尾 and COX-2, which may be more suitable for postoperative analgesia in patients with lung cancer.
【作者单位】：南通大学附属肿瘤医院麻醉科;南京医科大学公共卫生学院;
【基金】：南通市市级科技计划(指导性,HS13928)
【分类号】：R734.2

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