共轭亚油酸与运动对青春期肥胖大鼠肝脏及脂肪组织视黄醇结合蛋白4的影响

发布时间：2018-08-29 16:07

【摘要】：目的探讨共轭亚油酸(CLA)与运动对青春期肥胖大鼠肝脏及脂肪组织视黄醇结合蛋白4(RBP4)的影响。方法选取喂饲普通饲料的青春期SD大鼠8只为空白对照组(C),高脂组建模7周选取32只肥胖鼠随机分为4组,每组8只,分别为安静对照组(OC)、安静补CLA组(OCC)、运动组(OM)、运动结合CLA组(OMC)。动物跑台跑速为21~25 m/min,60 min/次;CLA灌胃1.6 g/kg,1次/日,5次/周,连续8周。实验结束后麻醉动物,采集血液及组织,测血糖、计算胰岛素敏感性,检测内脏脂肪组织RBP4 mRNA表达、肝脏组织RBP4蛋白表达及血浆RBP4浓度。结果干预后OM、OMC组体重、体重增长幅度和体脂百分比、血糖、内脏脂肪组织RBP4mRNA表达、肝脏组织RBP4蛋白表达、血浆RBP4浓度低于OC、OCC组(P0.01)。OM、OMC组胰岛素敏感性高于OC、OCC组(P0.01)。OMC组体重、体重增长幅度、胰岛素敏感性高于OM组,体脂百分比、血糖、RBP4表达低于OM组,但差异无统计学意义。结论单纯运动与运动结合CLA显著降低青春期肥胖大鼠的体重、体重增长幅度、体脂百分比、血糖,提高胰岛素敏感性及降低内脏脂肪组织RBP4的mRNA表达、肝脏组织RBP4蛋白表达、血浆RBP4水平,运动与运动结合CLA效果明显优于单纯补CLA。
[Abstract]:Objective to investigate the effects of conjugated linoleic acid (CLA) and exercise on retinol binding protein 4 (RBP4) in liver and adipose tissue of obese adolescent rats. Methods eight pubertal SD rats fed with normal diet were selected as blank control group, and 32 obese rats were randomly divided into 4 groups, 8 rats in each group, and 32 obese rats were randomly divided into 4 groups after 7 weeks of modeling in (C), hyperlipidemia group. (OCC), exercise group (OM), exercise group combined with CLA group (OMC). The running speed of animals was 21 ~ 25 m / min ~ 60 min/ d, 5 times a day and 5 times / week by gavage of 1.6 g / kg 路min ~ (-1) 路min ~ (-1) / d for 8 weeks. After the experiment, the anesthetized animals were collected, blood glucose was measured, insulin sensitivity was calculated, RBP4 mRNA expression in visceral adipose tissue, RBP4 protein expression in liver and plasma RBP4 concentration were detected. Results body weight, weight gain and body fat percentage, blood glucose, RBP4mRNA expression in visceral adipose tissue, RBP4 protein expression in liver tissue and plasma RBP4 concentration in OM,OMC group were lower than those in OC,OCC group (P0.01). The insulin sensitivity of OMN group was higher than that of OC,OCC group (P0.01). The weight gain, insulin sensitivity, body fat percentage, blood glucose and RBP4 expression in OM group were lower than those in OM group, but the difference was not statistically significant. Conclusion combined exercise and exercise combined with CLA can significantly reduce the body weight, weight gain, body fat percentage, blood glucose, insulin sensitivity, RBP4 mRNA expression in visceral adipose tissue, and RBP4 protein expression in liver tissue. The effect of plasma RBP4, exercise and exercise combined with CLA is better than that of CLA. alone.
【作者单位】：安徽师范大学体育学院;
【基金】：安徽省高校自然科学基金重点项目(No.KJ2013A138)
【分类号】：R589.2;Q591

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