基于液质联用的代谢组学方法研究天冬滋补保健作用的活性成分

发布时间：2018-09-04 13:46

【摘要】：目的:基于液质联用的代谢组学方法探索天冬的滋补保健作用的活性成分。方法:健康Wistar大鼠(雌雄各半)24只随机分对照组和给药组。给药组以3.0 gsIkg~(-1)sId~(-1)灌胃天冬,正常组用蒸馏水进行灌胃,连续42 d后麻醉,腹主动脉取血,分离血清,以超高效液相色谱-质谱联用技术(UPLC-MS)与主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)相结合检测血清,筛选出与滋补保健有关的内源性生物标记物。结果:在PCA与OPLS-DA模型中,正常组与给药组大鼠血清样本均有显著差异,跟正常组比较给药组共发现5个与滋补保健有关的内源性生物标记物,其中正离子发现5,6-trans-Vitamin D3、去氧皮质酮等两种内源性生物标记物,负离子发现亚油酸、二十二碳六烯酸、硬脂酸等三种内源性生物标记物,且所有标记物的含量均升高。结论:所发现的内源性生物标记物均为机体的有益活性成分,为天冬当的保健作用提供了理论依据。
[Abstract]:Objective: to explore the active components of the nourishing and health care of Aspergillus asparagus based on the method of liquid-mass-mass spectrometry. Methods: 24 healthy Wistar rats (male and female) were randomly divided into control group and administration group. The rats in the administration group were treated with 3.0 gsIkg~ (-1) sId~ (-1) intragastric perfusion, and the normal group were perfused with distilled water for 42 days after anesthesia, blood was taken from the abdominal aorta and serum was separated. The serum was detected by UPLC-MS and (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA). The endogenous biomarkers related to nourishing and health care were screened out. Results: in the model of PCA and OPLS-DA, there were significant differences in serum samples between normal group and administration group. Compared with normal group, five endogenous biomarkers related to nourishing and health care were found in the administration group. Two endogenous biomarkers, including positive ion discovery 5 ~ (6) -trans-Vitamin D _ 3, deoxycorticosterone, anion linoleic acid, 22 carbohexaenoic acid and stearic acid, were found, and the contents of all the markers were increased. Conclusion: all the endogenous biomarkers are beneficial active components and provide theoretical basis for the health care of asparagus.
【作者单位】：内蒙古民族大学附属医院;内蒙古民族大学蒙医药学院;内蒙古民族大学化学化工学院;
【基金】：内蒙古自治区科技创新引导项目(编号:YDJ20150025) 内蒙古自治区蒙医药科技重大专项子课题(编号:GCY201508034)
【分类号】：R285.5

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